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Chapter 20 DNA Technology Flashcards

Chapter 20: DNA Technology and Genomics Vocab

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135427784recombinant DNADNA derived from two different sources
135427785genetic engineeringthe direct manipulation of genes
135427786Biotechnologythe manipulation of organisms or their components to make useful products
135427787gene cloningThe process of preparing well defined, gene sized pieces of DNA in multiple identical copies
135427788cloneidentical cell
135427789restriction enzymesenzymes that cut DNA at a specific sequence of nucleotides
135427790sticky endan end of DNA in which one strand of the double helix extends a few units beyond the other
135427791DNA ligaseEnzyme that catalyzes t he formation of covalent bonds in the sugar-phosphate backbone
135427792restriction sitethe specific site at which a restriction enzyme will cleave DNA
135427793restriction fragmentsDNA segments resulting from cutting of DNA by a restriction enzyme
135427794cloning vectorthe original plasmid
137674553nucleic acid hybridizationa gene is detected by its ability to base pair with a complementary sequence.
137674554nucleic acid probeshort strand of RNA or DNA (single strand). hydrogen bonds with gene in nucleic acid hybridization. labeled with radioactive or fluorescent tag.
137674555denaturationthe separation of the two strands of DNA in a cell.
137674556genomic librarythe complete set of plasmid clones, each carrying copies of a particular segment from the initial genome.
137674557complementary DNAcDNA. double stranded DNA.
137674558cDNA librarycollection of cDNA clones, only part of the genome.
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