1.3 - Restriction Enzymes Flashcards

2901042978	foreign DNA	Restriction enzymes prevent infection from:	0
2901043770	prokaryotic	Restriction enzymes are only found in this type of cells:	1
2901045055	bacteriophages	A common vector of foreign DNA to bacterial cells are:	2
2901046430	lytic cycle	Bacteriophages infect, reproduce within, and then destroy bacterial cells by this process:	3
2901046924	endonucleases	Restriction enzymes are also termed:	4
2901050129	cutting	Restriction enzymes attack foreign DNA by recognizing and ________ it at certain sequences.	5
2901051015	4,6	Restriction enzymes recognize specific sequences that are __ or __ base pairs long.	6
2901052390	restriction sites	Sequences which a restriction enzyme reliably cuts are termed:	7
2901054648	genus and species of origin	The first three letters of a restriction enzyme refer to:	8
2901056699	water, buffer, dna, restriction enzyme	A typical restriction digest consists of these parts:	9
2901058519	enzyme cofactors	The buffer used for a digest controls the pH and provides:	10
2901059889	37C	The optimal temperature for most restriction enzymes is:	11
2901060468	sticky ends, blunt ends	There are two types of digest products:	12
2901084941	sticky ends	In this graphic, which type of digest product is indicated by A?	13
2901084942	blunt ends	In this graphic, which type of digest product is indicated by B?	14
2901122485	hybridize	Sticky end products are much easier to:	15
2901124019	DNA ligase	This enzyme joins digest fragments to form recombinant molecules:	16
2901126991	recombinant	DNA produced by hybridization, which is pieced together from different sources is:	17
2901129176	flexible	While blunt ends are more difficult to join chemically, they are also more ____ in their use.	18
2901130460	bacterial plasmid	Large amounts of a desired DNA sequence or protein can be produced by ligating it into a(n) __________ and allowing the host cell to replicate it.	19
2901180717	restriction map	A(n) ______ can be produced by subjecting a plasmid to several restriction enzymes and then comparing the sizes of the fragments produced.	20
2901181273	electrophoresis	This is a method of separating DNA fragments:	21
2901181706	size, charge	Electrophoresis separates fragments by ____ and ____.	22
2901182475	point mutations on restriction sites	Restriction digest can be used in the clinical lab to detect:	23
2901187654	electrophoresis, transfer to membrane	Blot tests consist of two parts:	24
2901184447	membrane	In a blot test, DNA is separated by electrophoresis and then wicked onto a(n):	25
2901186026	probe	In molecular biology, the term _____ refers to a sequence of ss-DNA which is labeled with an enzyme, luminescent chemical, or radioisotope.	26
2901187119	hybridize	In a blot test, labeled probes _____ with their complimentary strand, which allows the bands to be visualized.	27
2901189256	southern blot	This test involves electrophoresis, blotting, and labeling to detect DNA:	28
2901191272	northern blot	This test involves electrophoresis, blotting, and labeling to detect RNA:	29
2901192268	western blot	This test involves electrophoresis, blotting, and labeling to detect protein:	30