Campbell Biology Chapter 20 DNA technology Flashcards
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| 8461803944 | recombinant DNA | A DNA molecule made in vitro with segments from different sources. |  | 0 |
| 8461803945 | genetic engineering | The direct manipulation of genes for practical purposes. |  | 1 |
| 8461803946 | biotechnology | The manipulation of living organisms or their components to produce useful products. |  | 2 |
| 8461803947 | plasmid | A small ring of DNA that carries accessory genes separate from those of a bacterial chromosome; also found in some eukaryotes, such as yeast. |  | 3 |
| 8461803948 | gene cloning | The production of multiple copies of a gene. |  | 4 |
| 8461803949 | restriction enzymes | A degradative enzyme that recognizes and cuts up DNA (including that of certain phages) that is foreign to a bacterium; an important tool in biotechnology |  | 5 |
| 8461803950 | restriction sites | A specific sequence on a DNA strand that is recognized as a cut site by a restriction enzyme. |  | 6 |
| 8461803951 | sticky end | A single-stranded end of a double-stranded DNA restriction fragment. |  | 7 |
| 8461803952 | DNA ligase | A linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain; useful in forming recombinant DNA |  | 8 |
| 8461803953 | cloning vector | An agent used to transfer DNA in genetic engineering. A plasmid that moves recombinant DNA from a test tube back into a cell is an example of a cloning vector, as is a virus that transfers recombinant DNA by infection. |  | 9 |
| 8461803954 | complementary DNA | A DNA molecule made in vitro using mRNA as a template and the enzyme reverse transcriptase. this DNA molecule therefore corresponds to a gene, but lacks the introns present in the DNA of the genome. |  | 10 |
| 8461803955 | nucleic acid probe | In DNA technology, a labeled single-stranded nucleic acid molecule used to tag a specific nucleotide sequence in a nucleic acid sample. Molecules of the probe hydrogen-bond to the complementary sequence wherever it occurs; radioactive or other labeling of the probe allows its location to be detected. |  | 11 |
| 8461803956 | nucleic acid hybridization | Base pairing between a gene and a complementary sequence on another nucleic acid molecule. |  | 12 |
| 8461803958 | polymerase chain reaction (PCR) | A technique for amplifying DNA in vitro by incubating with special primers, taq polymerase molecules, and nucleotides. |  | 13 |
| 8461803959 | gel electrophoresis | The separation of nucleic acids on the basis of their size and electrical charge, due to their different rate of movement through an electrical field in a gel. |  | 14 |
| 8461803960 | restriction fragment length polymorphism (RFLP) | Differences in DNA sequence on homologous chromosomes that result in different patterns of restriction fragment lengths (DNA segments resulting from treatment with restriction enzymes); . |  | 15 |
| 8461803962 | dideoxy chain termination method | a DNA sequencing method in which target DNA is denatured and annealed to an oligonucleotide primer, which is then extended by DNA polymerase using a mixture of deoxynucleotide triphosphates (normal dNTPs) and chain-terminating dideoxynucleotide triphosphates (ddNTPs) |  | 16 |
| 8461803965 | DNA microarray assay | A method to detect and measure the expression of thousands of genes at one time. Tiny amounts of a large number of single-stranded DNA fragments representing different genes are fixed to a glass slide. These fragments, ideally representing all the genes of an organism, are tested for hybridization with various samples of cDNA molecules. |  | 17 |
| 8461803967 | RNA interference | A technique to silence the expression of selected genes in nonmammalian organisms. The method uses synthetic double-stranded RNA molecules matching the sequence of a particular gene to trigger the breakdown of the gene's messenger RNA or block its translation;useful for determining gene function |  | 18 |
| 8461803968 | single-nucleotide polymorphism (SNP) | One base-pair variation in the genome sequence; useful as markers for diseases in the human population |  | 19 |
| 8461803969 | reverse transcriptase | An enzyme encoded by some certain viruses (retroviruses) that uses RNA as a template for DNA synthesis. |  | 20 |
| 8461803975 | epigenetic changes | methylation of DNA and histones and other types of chromatin modification that need to be reversed in reprogramming of a differentiated cell for organismal cloning to be successful |  | 21 |