Biochemistry Flashcards

8488847276	Gs	stimulates adenylate cyclase, which increases levels of cAMP in the cell	0
8488851303	Gi	inhibits adenylate cyclase, which decreases levels of cAMP in the cell	1
8488856531	Gq	activates phospholipase C, which cleaves a phospholipid from the membrane to form PIP2. PIP2 is then cleaved into DAG and IP3. IP3 can open calcium channels in the ER, increasing Calcium levels in the cell.	2
8488883858	G protein-coupled receptors	large family of integral membrane proteins involved in signal transduction.	3
8488889974	Enzyme-linked receptors	membrane receptors with a catalytic activity. 3 primary protein domains: membrane-spanning, ligand-binding and catalytic	4
8488937159	Electrophoresis	moves proteins according to charge and sz	5
8488950515	Native PAGE	mass-to-charge or mass-to-sz ratios. Protein can be recovered.	6
8488959919	SDS- PAGE	Separates proteins on the basis of molecular mass alone. SDS covers entire protein in negative charge	7
8488972867	What is the average molar mass of one amino acid?	100 daltons or 100g/mol	8
8488980470	Isoelectric focusing	separating on the basis of pI. Say you have a protein with the pI of 9(basic)	9
8489012204	When is chromatography preferred over electrophoresis?	When large amounts of protein are being separated	10
8489078129	X-ray crystallography	the most reliable and common method of identifying protein structure(75%). measures electron density on an extremely high resolution scale and can also be used for nucleic acids.	11
8489088885	NMR spectroscopy	to find 25% of protein structures-	12
8489097094	Edman degradation	small proteins best analyzed. 50-70 aa. selectively and sequentially removes the N-terminal aa which can then be analyzed via mass spec	13