AP Biology - Chapter 20 Flashcards

9531286126	Recombinant DNA	DNA produced by combining DNA from different sources	0
9531286127	Biotechnology	A form of technology that uses living organisms, usually genes, to modify products, to make or modify plants and animals, or to develop other microorganisms for specific purposes.	1
9531286128	Plasmids	Small rings of DNA found naturally in some bacterial cells in addition to the main bacterial chromosome. Can contain genes for antibiotic resistance, or other "contingency" functions.	2
9531286129	Gene Cloning	The production of multiple copies of a gene.	3
9531286130	Restriction enzymes	Enzyme that cuts DNA at a specific sequence of nucleotides	4
9531286131	Sticky end	A single-stranded end of a double-stranded DNA restriction fragment.	5
9531286132	DNA Ligase	A linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain.	6
9531286133	Cloning vector	DNA molecules that can carry foreign DNA into a host cell and replicate there.	7
9531286134	Complimentary DNA (cDNA)	is made from mRNA by reverse transcriptase	8
9531286135	polymerase chain reaction (PCR)	A method of producing thousands of copies of DNA segment using the enzyme DNA polymerase	9
9531286136	gel electrophoresis	Procedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel	10
9531286137	southern blotting	A hybridization technique that enables researchers to determine the presence of certain nucleotide sequences in a sample of DNA.	11
9531286138	reverse transcriptase- PCR	An enzyme encoded by some certain viruses (retroviruses) that uses RNA as a template for DNA synthesis.	12
9531286139	Genetic profile	An individual's unique set of genetic markers, detected most often today by PCR or, previously, by electrophoresis and nucleic acid probes.	13