GWHS AP Biology - Chapter 20: Biotechnology Flashcards

8275342783	recombinant DNA	A DNA molecule made in vitro with segments from different sources.	0
8275342784	genetic engineering	The direct manipulation of genes for practical purposes.	1
8275342785	biotechnology	The manipulation of living organisms or their components to produce useful products.	2
8275342786	plasmid	A small ring of DNA that carries accessory genes separate from those of a bacterial chromosome; also found in some eukaryotes, such as yeast.	3
8275342787	gene cloning	The production of multiple copies of a gene.	4
8275342788	restriction enzymes	A degradative enzyme that recognizes and cuts up DNA (including that of certain phages) that is foreign to a bacterium; an important tool in biotechnology	5
8275342789	restriction sites	A specific sequence on a DNA strand that is recognized as a cut site by a restriction enzyme.	6
8275342790	sticky end	A single-stranded end of a double-stranded DNA restriction fragment.	7
8275342791	DNA ligase	A linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain; useful in forming recombinant DNA	8
8275342792	cloning vector	An agent used to transfer DNA in genetic engineering. A plasmid that moves recombinant DNA from a test tube back into a cell is an example of a cloning vector, as is a virus that transfers recombinant DNA by infection.	9
8275342793	complementary DNA	A DNA molecule made in vitro using mRNA as a template and the enzyme reverse transcriptase. this DNA molecule therefore corresponds to a gene, but lacks the introns present in the DNA of the genome.	10
8275342794	nucleic acid probe	In DNA technology, a labeled single-stranded nucleic acid molecule used to tag a specific nucleotide sequence in a nucleic acid sample. Molecules of the probe hydrogen-bond to the complementary sequence wherever it occurs; radioactive or other labeling of the probe allows its location to be detected.	11
8275342795	nucleic acid hybridization	Base pairing between a gene and a complementary sequence on another nucleic acid molecule.	12
8275342796	electroporation	A technique to introduce recombinant DNA into cells by applying a brief electrical pulse to a solution containing cells. The electricity creates temporary holes in the cells' plasma membranes, through which DNA can enter.	13
8275342797	polymerase chain reaction (PCR)	A technique for amplifying DNA in vitro by incubating with special primers, taq polymerase molecules, and nucleotides.	14
8275342798	gel electrophoresis	The separation of nucleic acids on the basis of their size and electrical charge, due to their different rate of movement through an electrical field in a gel.	15
8275342799	restriction fragment length polymorphism (RFLP)	Differences in DNA sequence on homologous chromosomes that result in different patterns of restriction fragment lengths (DNA segments resulting from treatment with restriction enzymes); .	16
8275342800	Southern Blotting	A hybridization technique that enables researchers to determine the presence of certain nucleotide sequences in a sample of DNA.	17
8275342801	dideoxy chain termination method	a DNA sequencing method in which target DNA is denatured and annealed to an oligonucleotide primer, which is then extended by DNA polymerase using a mixture of deoxynucleotide triphosphates (normal dNTPs) and chain-terminating dideoxynucleotide triphosphates (ddNTPs)	18
8275342802	reverse transcriptase-polymerase chain reaction (RT-PCR)	a variant of polymerase chain reaction (PCR), is a technique commonly used in molecular biology to detect mRNA expression; useful for studying gene expression during development	19
8275342803	northern blotting	an adaptation of the Southern blot procedure used to detect specific sequences of RNA by hybridization with complementary DNA.	20
8275342804	DNA microarray assay	A method to detect and measure the expression of thousands of genes at one time. Tiny amounts of a large number of single-stranded DNA fragments representing different genes are fixed to a glass slide. These fragments, ideally representing all the genes of an organism, are tested for hybridization with various samples of cDNA molecules.	21
8275342805	in vitro mutagenesis	The creation of mutations in genes; used to knock out genes and then determine their function	22
8275342806	RNA interference	A technique to silence the expression of selected genes in nonmammalian organisms. The method uses synthetic double-stranded RNA molecules matching the sequence of a particular gene to trigger the breakdown of the gene's messenger RNA or block its translation;useful for determining gene function	23
8275342807	single-nucleotide polymorphism (SNP)	One base-pair variation in the genome sequence; useful as markers for diseases in the human population	24
8275342808	reverse transcriptase	An enzyme encoded by some certain viruses (retroviruses) that uses RNA as a template for DNA synthesis.	25
8275342809	CRISPR-cas 9	a system used by bacteria to defend against bacteriophage infections; used in genetic engineering to target and disable genes and thereby determine their function	26
8275342810	totipotent	a cell that can give rise to all specialized cell types; in animals that includes the placenta	27
8275342811	pluripotent	embryonic stem cells from the blastocyst that can give rise to all specialized cell types but not the placenta	28
8275342812	induced pluripotent cells	iPS differentiated cells that are reprogrammed to become pluripotent that can be used to study and treat disease	29
8275342813	somatic cell nuclear transfer	used in organismal cloning; nucleus of a somatic cell replaces the nucleus of an enucleated egg	30
8275342814	epigenetic changes	methylation of DNA and histones and other types of chromatin modification that need to be reversed in reprogramming of a differentiated cell for organismal cloning to be successful	31