Biotechnology and it's processes Flashcards

11127842087	herbert boyer was bron in	1936	0
11127842088	the European Federation of Biotechnology	the integration of natural science and organisms cells parts thereof and molecular analogues for products and services	1
11127842089	genetic engineering	techniques to alter the chemistry of genetic material dna and rna to introduce these into gost and thus change the phenotype	2
11127842090	Bioprocess Technology	maintenance of sterile ambience in chemical engineering process to enable growth of only desired microbe	3
11127842091	origin of replication	initiation of replication	4
11127842092	Cloning	making multiple identical copies of any template dna	5
11127842093	Plasmid	autonomously replicating circular extra Chromosomal dna	6
11127842094	1st plasmid	salmonella typhimurium	7
11127842095	Stanley Cohen and herbert boyer in	1972	8
11127842096	normal ecoli is	not antibiotic resistant	9
11127842097	restriction enzyme	smith and nathan	10
11127842098	bacteria protect themselves from viruses	by fragmenting viral dna with endonuclease	11
11127842099	Molecular scissors	restriction enzymes	12
11127842100	endonuclease is	nuclease	13
11127842101	1963	two enzymes were discovered in	14
11127842102	1 endonuclease	hind \|\|	15
11127842103	methyl group	enzyme endonuclease cannot cut it's own DNA bcoz	16
11127842104	ecoli strian	R	17
11127842105	removed dna from ends	exonuclease	18
11127842106	removes dna at specific points	endonuclease	19
11127842107	palindromic nucleotide sequences	each restriction endonuclease recognize a specific	20
11127842108	cohesive ends	sticky ends	21
11127842109	gel electrophoresis	dna fragments are separated	22
11127842110	agarose gel	sea weed	23
11127842111	using ethidium bromide	dna is seen	24
11127842112	the ser fragments	move farther	25
11127842113	the separated bands of dna are cut out from the agarose gel and extracted from the gel piece	elution	26
11127842114	transformation	process through which price of dna is introduces in a host bacteria	27
11127842115	terR	BamH I Sal I	28
11127842116	Bam HI	GGATCC	29
11127842117	Bam H1	Bacillus amyloliquefaciens	30
11127842118	ampR	Pvu I Pst T	31
11127842119	insertional inactivation	inactivation of gene synthesis of that enzyme	32
11127842120	when big dna	BAC YAC	33
11127842121	ss bacteriophage	m13 vector	34
11127842122	ds bacteriophage	lambda phage	35
11127842123	PUC	plasmid university of California	36
11127842124	chromatogenic substance	change into blue by beta galactosidase	37
11127842125	chromatogenic no color	a galactosidase	38
11127842126	Agrobacterium tumefaciens	vector for ti plasmid	39
11127842127	pcr by	kary mullis	40
11162407171	gel electrophoresis by	tiselius	41