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Basics of Genetics

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Biology
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Genetics

2 of 13 PCR. Polymerase chain reaction is used to copy DNA. Cycle 1: - A sample of DNA is added to detergent to release the DNA from cells. - DNA polymerase, DNA primers with fluorescent markers and nucleotides are added. - (at 95 degrees) the DNA splits into two strands. - (at 55 degrees) primers attach at the start of the STR repeated sequence. - (at 70 degrees ) DNA polymerases attach nucleotides are added the DNA sequence is replicated. 1 of 13 DNA profiling. Non coding blocks in DNA are called introns, and the coding regions are called exons. In introns DNA sequences are repeated many times, this is called short tandem repeats (STR's). A DNA profile is produced using gel electrophoresis, In which DNA fragments produced by

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