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8563875912Dehydrationconnecting monomers together by the removal of water0
8563875913Hydrolysisdisassembling polymers by the addition of water1
8563875914Disaccharidesglucose + glucose = maltose / glucose + fructose = sucrose / glucose + galactose = lactose2
8563875915PolysaccharidesPlants: starch (energy) and cellulose (structure) Animals: glycogen (energy) and chitin (structure)3
8563875916*Lipidshydrophobic (very non-polar), consist of long hydrocarbon chains4
8563875917Fatsconsist of glycerol and 3 fatty acids, store long term energy, saturated = no double bond in hydrocarbon tails (no kink), unsaturated = double bond (kink)5
8563875918Phospholipidsconsist of phosphate head, glycerol, and 2 fatty acid tails, tail is hydrophobic, head is hydrophillic6
8563875919Protein structure and organizationcomposed of an amino group, a carboxyl group, hydrogen, and an R group, joined by peptide bonds and folded numerous times; 1) Primary (linear sequence) 2) Secondary (helix or pleat) 3) Tertiary 4) Quaternary (globular)7
8563875920Protein functions (8)1) enzymes 2) antibodies 3) storage proteins 4) transport proteins 5) hormones 6) receptor proteins 7) motor proteins 8) structural proteins8
8563875921*Nucleic AcidsDNA (A+T, G+C) carries genetic info, RNA (A+U, G+C) manufactures proteins9
8563875922Nuclear Envelopedouble membrane enclosing the nucleus (where genetic info is stored) perforated with pores, continuous with ER10
8563875923Chromatinuncondensed DNA that forms chromosomes during cell division11
8563875924Nucleolusnonmembranous structure involved in production of ribosomes, a nucleus has one or more of these12
8563875925Rough ERcovered in ribosomes, secretes and transports proteins produced by ribosomes13
8563875926Smooth ERmetabollic processes (synthesis of lipids, metabolism of carbs, detoxification of drugs and poisons)14
8563875927Golgistores, transports, and secretes cell products15
8563875928Cytoskeletonsupports cell, maintains its shape, aids in movement of cell products16
8563875929Centrosomes (2 centrioles)only in animal cells, microtubules used for cell division17
8563875930Lysosomesonly in animal cells, digestive organelles18
8563875931Flagellaonly in animal cells, cluster of microtubules for motility19
8563875932Extracellular Matrixonly in animal cells, made of proteins that provide support for cells and relay information for communication between the environment and the cell20
8563875933Central Vacuoleonly in plant cells, stores water and sugar, breaks down waste, and used as a mechanism for plant growth (when it swells)21
8563875934Prokaryotic vs. Eukaryoticnucleoid / nucleus; only ribosomes / complex membrane-bound organelles; both have same genetic coding, sugars, and amino acids22
8563875935Phospholipid Bilayertails of phospholipids are loosely packed and are in constant motion; membrane contains integral and peripheral proteins, cholestrol, and glycopreotins and glycolipids; cholesterol makes the membrane less permeable to water and other substances; non-polar and small polar molecules can pass through unadied23
8563875936Passive trasportmovement of molecules without requirement of energy: 1) diffusion 2) osmosis (across a membrane) 3) facilitated diffusion (helped by transport proteins)24
8563875937Active transportmovement of molecules that requires energy: 1) sodium-potassium pumps 2) exocytosis 3) endocytosis (phagocytosis, pinocytosis)25
8563875938Membrane Potentialvoltage across a membrane due to difference in positive and negative ions, electrons move from high to low concentration (ex. sodium-potassium pumps in neurons)26
8563875939Electrochemical Gradientdiffusion gradient resulting in combination of membrane potential and concentration gradient27
8563875940Hypertonicsolution with higher concentration of solutes, animal/plant cell in this solution would become shiveled/plasmolyzed28
8563875941Hypotonicsolution with lower concentration of solutes, animal/plant cell in this solution would lyse/become turgid29
8563875942Isotonicequal levels of solute concentration, plant cell in this solution would become flaccid30
8563875943When ΔG is negative......the reaction is exergonic (loss of free energy).31
8563875944When ΔG is positive......the reaction is endergonic (gain of free energy).32
8563875945*Enzymesproteins that are biological catalysts, lower the activation energy required to start a chemical reaction (reactants at unstable transition state) can be used over and over33
8563875946Substratethe substance that an enzyme acts upon34
8563875947Active Siteregion of enzyme that binds to the substrate35
8563875948Induced fitchange in the shape of an enzyme's active site induced by the substrate, helps to break down the substrate36
8563875949The higher the substrate concentration......the faster the reaction until the enzyme becomes saturated.37
8563875950Denaturationthe unraveling of an enzyme due to high temperatures or incompatible pH38
8563875951Cofactorsnonprotein molecules that are required for proper enzyme function, cofactors made of organic molecules are called coenzymes39
8563875952Enzyme inhibition may be irreversible if......the inhibitor attaches by covalent bonds (poisons, toxins)40
8563875953Competitive Inhibitorsresemble a substrate and block enzymes' active sites, can be overcome with higher concentration of substrate41
8563875954Noncompetitive Inhibitorsbind to a portion of the enzyme and change the shape of the active site so that it cannot match with substrates, used for regulating metabolic reactions42
8563875955Feedback Inhibitionthe product of a metabolic pathway switches off the enzyme that created it earlier in the process43
8563875956Oxidationloss of electrons (OIL)44
8563875957Reductiongain of electrons (RIG)45
8563875958Oxidative PhosphorylationATP synthesis powered by redox reactions that transfer electrons to oxygen46
8563875959Electron AcceptorsCellular respiration: NAD+ and FAD (to NADH and FADH2) Photosynthesis: NADP+ (to NADPH)47
8563875960GlycolysisInput: glucose, 2 ATP Output: 2 pyruvic acid, 4 ATP (net 2), 2 NADH48
8563875961Conversion Reaction before Kreb'sInput: 2 pyruvate Output: 2 acetyl (w/ CoA), 2 NADH, 2 CO249
8563875962Krebs CycleInput: 2 acetyl ➝ citric acid Output: 2 ATP, 6 NADH, 2 FADH2, 4 CO2 (after 2 turns of the cycle)50
8563875963Electron Transport ChainInput: NADH, FADH2, O2 (to accept e-) Output: 34-38 ATP, H2O51
8563875964Alcohol FermentationInput: glucose, 2 ATP, 2 NADH Output: 2 NAD+, 2 ethanol, 2 CO2, 4 ATP (net 2)52
8563875965Lactic Acid FermentationInput: glucose, 2 ATP, 2 NADH Output: 2 NAD+, 2 lactate, 4 ATP (net 2)53
8563875966Photosynthetic Equation54
8563875967Chloroplast structureExciting chlorophyll: chlorophyll in thylakoids absorb light, which excites electrons to produce potential energy55
8563875968Light ReactionsInput: H2O (2 e-), light energy, NADP+ Output: O2, ATP, NADPH56
8563875969Calvin CycleInput: 6 CO2 (fixed to RuBP by Rubisco), ATP, NADPH Output: 2 G3P = 1 glucose57
8563875970Watson and Crickbuilt the first accurate 3D DNA model58
8563875971Leading Strand vs. Lagging Strandworks toward replication fork / works away from replication fork; both always move in the 5' ➝ 3' direction59
8563875972Steps of DNA Replication1) helicase separates the DNA strands 2) SSB proteins prevent DNA from reanneling 3) primase creates RNA primer 4) DNA polymerase extends DNA strand from the primer 5) DNA polymerase I (RNase H) removes the primers 6) ligase joins the okazaki fragments of the lagging strand60
85638759733 types of RNA1) mRNA messenger 2) tRNA transfer amino acids (20 kinds) 3) rRNA ribosomes61
8563875974Transcription1) Initiation: promoter site (TATA) is recognized 2) Elongation: RNA polymerase adds ribonucleotides in the 5' ➝ 3' direction 3) Termination: RNA strand separates, RNA polymerase recognizes termination sequence (AAUAAA)62
8563875975RNA processing/splicingsplicesomes remove introns and put together exons, 5' cap and PolyA tail are added63
8563875976Codon vs. Anticodoncodon = nucleotide sequence on mRNA anticodon = nucleotide sequence on tRNA64
8563875977Translation1) Initiation: 5' cap attaches to ribosome which accepts an initiator tRNA at the P site (*AUG will always be 1st codon) 2) Elongation: codon/anticodon recognition and formation of peptide bond between A site amino acid and P site amino acid chain 3) translocation of the ribosome down the mRNA strand 4) Termination: ribosome will recognize stop codon and release the protein65
8563875978DNA mutationsbase-pair substitution; insertion/deletion; frameshift: 1) missense = different protein 2) nonsense = codes for a stop signal prematurely 3) silent = no harmful change66
8563875979Prokaryotic cell divisionbinary fission: splits in 2, exact copies, quick and efficient with few mutations, but reduces amount of genetic variation67
8563875980Somatic cell vs. Gameteany body cell except gametes / reproductive cells (sperm, egg)68
8563875981Interphase(90% of cell's life) G1: 1st growth, normal metabolic activity (goes into G0 phase if it is not ready for next phase); S: synthesis, DNA replication; G2: 2nd growth, prepares for mitosis69
8563875982Mitosis1) Prophase: chromatin condenses into chromosomes, nucleus disappears 2) Metaphase: chromosomes line up at equator, kinetechore microtubules attach 3) Anaphase: sister chromatids move to opposite poles of the cell 4) Telophase and Cytokinesis: daughter cells separate, nucleus reforms, chromosomes decondense70
8563875983Cyclin-dependent Kinases (Cdks)a regulatory protein that depends upon the presence of cyclin to complete its function, MPF is a Cdk that triggers a cell's passage into the M phase71
8563875984Meiosis I1) Prophase I: homologous chromosomes pair up and synapsis occurs, crossing over segments of the chromosomes (chiasma) to create more genetic variation 2) Metaphase I: homologous chromosomes line up at the equator 3) Anaphase: homologous chromosomes move to opposite poles of the cell. 4) Telophase I...72
8563875985Meiosis IIProphase II - Telophase II act exactly like mitosis except that the resultant number of daughter cells is 4 instead of 2, each with their own unique combination of genetic information73
85638759864 mechanisms that contribute to genetic variation1) Mutation 2) Independent Assortment: homologous chromosomes align randomly on one side of the equator or another 3) Crossing Over 4) Random Fertilization: a zygote can be any combination of a sperm and egg (64 trillion different combinations in humans)74
8563876011Repressible Operontrp operon - usually on, can be repressed. Repressor protein produced in inactive shape75
8563876012Inducible Operonlac operon - usually off, can be turned on. Repressor protein produced in active shape.76
8563876013cAMP and CAP regulated Operonwhen CAP is inactive, transcription continues at a much less efficient rate even in the presence of lactose77
8563876015Histone Methylationthe condensing of chromatin structure (heterochromatin), prevents transcription78
8563876016Transcription Factors and EnhancersRNA polymerase requires the assistance of transcription factor proteins and enhancers or activators to successfully transcribe RNA79
8563876017Epigenetic Inheritanceinheritance of traits not directly related to nucleotide sequence (ex. diet and environment contribute to characteristics without changing DNA sequence)80
8563876056Plasmidsa small, circular, double-stranded DNA molecule that carries accessory genes separate from those of a bacterial chromosome81
8563876057Recombinant DNAa DNA vector made in vitro with segments from different sources82
8563876058Restriction Enzymean enzyme that recognizes and cuts DNA molecules at specific nucleotide sequences (restriction sites), can then be used to create recombinant DNA83
8563876059Gel Electrophoresisanalyzing fragments of DNA (RFLPs) by their length and charge to determine genetic fingerprints and other genetic information84

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