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AP Biology Semester 1 Flashcards

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607701431Bioenergeticsthe study of how organisms manage their energy resources
607701432metabolismthe sum of an organism's chemical reactions -the chemistry of life is organized into metabolic pathways and these pathways alter molecules (catabolic/anabolic)
607701433catabolicrelease energy stored in complex molecules by breaking htem down into simpler ex: cell respiration
607701434anabolicrequires/consumes energy to combine simpler molecules into more complicated ex: protein synthesis from amino acids, photosynthesis
607701435energythe capacity to cause change and is measured in Joules, calories
607701436thermodynamicsstudy of energy transformations law 1: energy can't be created or destroyed by is transferred and transformed between matter law 2: energy transfor causes more disorder in the universe (entropy)
607701437entropya quantity used as a measurement of heat (kinetic energy)
607701438free energythe portion of a system's energy that is able to perform work (available energy) G - measure of instability of system - more free energy, more unstable
607701439enthalpytotal energy
607701440ATPstructure in a nucleotide consisting of adenine, ribose, chain of 3 phosphate groups (adenosine triphosphate)
607701441energy couplingusing exergonic processes to power endergonic
607701442phosphorylateda phosphate group is transferred from ATP to some other molecule in order to make the second molecule less stable (ADP)
607701443enzymea protein catalyst that changes the reaction rate without being consumed by the reaction, allows substrate to reach transition state and provides environment so substrate can change -lowers activation energy so speeds up reaction
607701444substratean enzyme's reactant that fits into the active site
607701445active sitethe pocket on the enzyme's surface that is complimentary shaped for the substartae so the substrate can attach through weak chemical bonds (tertiary structure)
607701446induced fitconfirmational change in shape, enzyme molds around substrate like a handshake
607701447cofactorsinorganic element (ex: iron, mg in polymerase, trace elements) small elements that bind permanently or reversibly with enzymes and are needed for enzyme catalytic function and structure
607701448coenzymeslarge, organic, used to make most vitamins
607701449competitive inhibitorscompete with the substrate for the last active site (ex: hemoglobin and carbon monoxide)
607701450noncompetitive inhibitorsbind to another part of the enzyme and change its formation ex: flea medication and disables ability to make chitin/exoskeleton
607701451allosteric regulationbinding to a site separate from the active site and either inhibit or activate the enzyme
607701452cooperativityhow the induced fit binding of a substrate and a subunit change the conformation so the subunit's active sites are more active
607701453macromoleculesgiant molecules formed from smaller molecules usually by condensation reaction (carboyhydrates, lipids, proteins, nucleic acids)
607701454polymerlong molecule made up of many covalent bonds holding together similar or identical building blocks or monomers
607701455monosaccharidessugar monomers (glucose, fructose)
607701456disaccharidesugar dimers -sucrose (glu+fru) -maltose (glu+glu) -lactose (glu+gala)
607701457glycosidic linkageis a covalent bond with 2 monosaccharides by a dehydration reaction (ex: to glucose molecules to form maltose)
607701458polysaccharidecarbohydrate polymers (starch) amylose, glycogen, cellulose, chitin
607701459Lipidshave carbon, hydrogen, oxygen (triglyceride, fatty acids, phospholipid, steroid)
607701460amino acidorganic molecule with carboxyl and amino groups and a variable (R) group
607701461primary structureorder of amino acids determined by genetic info
607701462secondary structurealpha helix - coil by hydrogen bonding beta pleated sheet - core of globular proteins
607701463tertiary structureoverall polypeptide shape from R group interactiosn, hydrophobic interactions, disulfide bridges
607701464quaternary structureprotein structure from polypeptide subunits
607701465antiparallelDNA strands because the opposite sugar phosphate arrangement in the double helix and the strands are parallel with one going 5' 3' and the other 3' 5'
607701466anaerobicpartial degredation of sugars in the absence of oxygen (early bacteria) ex: fermentation
607701467aerobicuses oxygen as a reactant to complete the breakdwon of organic molecules ex: cell respiration
607701468redox reactionsreactions that result in the transfer of one or more electrons from one reactant to another (OIL RIG) - loss of electrons is oxidation, electron recipient is oxidizing agent - addition of electrons is reduction, and electron donor is reducing agent cell respiration example of redox reaction
607701469glycolysisbreaking glucose into two molecules of pyruvate occurs in cytosol
607701470Krebs/ citric acid cycledegrades pyruvate to carbon dioxide occurs in mitochondrial matrix
607701471electron transport chainthe e- movement from cytochrom to another until they combine with oxygen and hydrogen ions to form water in inner membrane of mitochondria (cristae)
607701472oxidative phosphorylationproduction of ATP using energy derived from redox reactions of an ETS, whre electrons are passed to inner membrane of mitochondria ETS produces almost 90% of ATP
607701473substrate-level phosphorylationenzyme transfers a phosphate group from an organic molecule (substrate) to ADP forming ATP -occurs in glycolysis and krebs
607701474stromaopening so CO2 can go into the leaves and O2 can go out and water leaks out (transpiration) fewer stroma in cactus

AP Statistics - Lesson 1.2 Flashcards

from The Practice of Statistics, 4th edition, by Starnes, Yates, and Moore

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555852884dotplotindividual values shown as a dot above its location on a number line - shows distribution of a quantitative variable
555852887overall patternshape, center, spread
555852888outlierindividual value that falls outside the overall pattern
555852892describing a distribution of quantitative dataS- shape O- outliers C- center S- spread
555870117shapeskewed/symmetrical and mode
555852889mode; modesmost frequent; major peaks
555870118centermedian or mean
555852890meanaverage
555901642medianthe middle value of a data set when numbers are arranged from least to greatest
555901643spreadhow much variability in the data, range
555901644rangehighest value minus smallest value
555852894skewed rightright side of the graph with larger values is longer than the left
555901645skewed leftleft side of the graph is much longer than the right side
555852893symmetricthe right and left sides of the graph are almost mirror images
555852895unimodalsingle peak
555852896bimodaltwo clear peaks
555852897multimodalmultiple peaks
555852885stemplotseparate each observation into a stem and a one-digit leaf; show distribution of a quantitative variable
555901646split stemsstem repeated twice: first line leaf values of 0-4, second line leaf values of 5-9; ex: 0 | 0 1 2 3 4 0 | 5 6 7 8 9
555901647back-to-back stemplotcompare two variables, share same stem, work out from center on both sides
555852886histogramplot the counts (frequencies) or percents (relative frequencies) of values in equal-width classes; shows distribution of a quantitative variable

President #, Occupation, & Nickname Flashcards

Made by me. Source: the great Wikipedia!
Format:
Q: #President's name
A: nickname/occupation
Enjoy! For Mr. Thomas.

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4403695811George Washingtonthe father of his country / general
4403695822John Adamshis rotundity / lawyer
4403695833Thomas Jeffersonthe land of the people / lawyer
4403695844James Madisonhis little majesty / lawyer
4403695855James Monroethe era of good feelings president / lawyer
4403695866John Q. Adamsthe abolitionist / lawyer
4403695877Andrew Jacksonold hickory / soldier, lawyer
4403695888Martin van Burenthe enchanter / lawyer
4403695899William Harrisonold tippecanoe / soldier
44036959010John Tylerhis accidency / lawyer
44036959111James Polkyoung hickory / lawyer
44036959212Zachary Taylorold rough and ready / soldier
44036959313Millard Fillmorefriendly / lawyer
44036959414Franklin Pierceyoung hickory of the granite hills / lawyer
44036959515James Buchananold public functionary / lawyer
44036959616Abraham Linconhonest abe / lawyer
44036959717Andrew Johnsonthe tennessee tailor / tailor
44036959818Ulysses Grantunconditional surrender grant / general
44036959919Rutherford HayesRutherfraud / lawyer
44036960020James Garfieldboatman jim / soldier
44036960121Chester Arthurgentleman boss / lawyer
44036960222/24Grover Clevelanduncle jumbo / lawyer
44036960323Benjamin Harrisonyoung tippecanoe / lawyer
44036960425William Mckinleythe napoleon of protection / lawyer
44036960526Theodore Rooseveltthe lion / soldier
44036960627William Taftbig chief / lawyer
44036960728Woodrow Wilsonthe phrasemaker / lawyer
44036960829Warren Hardingnews publisher
44056815630Calvin Coolidgecool cal / lawyer
44056815731Herbert Hooverthe chief / engineer
44056815832Franklin RooseveltFDR / lawyer
44056815933Harry Trumangive em hell harry / farmer
44056816034Dwight Eisenhowerike / general
44056816135John F. KennedyJFK / sailor lieutenant
44056816236Lyndon JohnsonLBJ / teacher
44056816337Richard Nixontricky dick / sailor lieutenant
44056816438Gerald FordJerry / sailor lieutenant
44056816539Jimmy CarterJimmy / sailor lieutenant, lawyer
44056816640Ronald ReaganDutch / actor
44056816741George Hw Bushbush the elder / pilot
44056816842Bill Clintonbubba / lawyer
44056816943George W Bushdubya / businessman
44056817044Barack H Obamano drama obama / lawyer

AP Biology, Ch. 5-Macromolecules Flashcards

Campbell 7e
Structure & Function of Macromolecules

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577334821alpha helixA spiral shape constituting one form of the secondary structure of proteins, arising from a specific hydrogen-bonding structure.
577334822amino acidAn organic molecule possessing both carboxyl and amino groups. monomers of proteins.
577334823antiparallelAn arrangement found In DNA, where two sugar-phosphate backbones run in opposite 5' - 3' directions from each other. Bk
577334824beta (b) pleated sheetOne form of the secondary structure of proteins in which the polypeptide chain folds back and forth, or where two regions of the chain lie parallel to each other and are held together by hydrogen bonds.
577334825catalystA chemical agent that changes the rate of a reaction without being consumed by the reaction.
577334826celluloseA structural polysaccharide of cell walls, consisting of glucose monomers joined by b-1, 4-glycosidic linkages.
577334827chaperoninProtein molecules that assist the proper folding of other proteins.
577334828chitinA structural polysaccharide of an amino sugar found in many fungi and in the exoskeletons of all arthropods.
577334829cholesterolA steroid that forms an essential component of animal cell membranes and acts as a precursor molecule for the synthesis of other biologically important steroids.
577334830collagenA glycoprotein in the extracellular matrix of animal cells that forms strong fibers, found extensively in connective tissue and bone; the most abundant protein in the animal kingdom.
577334831condensation reactionA reaction in which two molecules become covalently bonded to each other through the loss of a small molecule, usually water; also called dehydration reaction.
577334832dehydration reactionA chemical reaction in which two molecules covalently bond to each other with the removal of a water molecule.
577334833denaturationFor proteins, a process in which a protein unravels and loses its native conformation, thereby becoming biologically inactive. For DNA, the separation of the two strands of the double helix. occurs under extreme conditions of pH, salt concentration, and temperature.
577334834deoxyribonucleic acid (DNA)A double-stranded, helical nucleic acid molecule capable of replicating and determining the inherited structure of a cell's proteins.
577334835deoxyriboseThe sugar component of DNA, having one less hydroxyl group than ribose, the sugar component of RNA.
577334836disaccharideA double sugar, consisting of two monosaccharides joined by dehydration synthesis.
577334837disulfide bridgeStrong covalent bonds formed when the sulfur of one cysteine monomer bonds to the sulfur of another cysteine monomer.
577334838double helixThe form of native DNA, referring to its two adjacent polynucleotide strands wound into a spiral shape.
577334839enzymeA protein serving as a catalyst, a chemical agent that changes the rate of a reaction without being consumed by the reaction.
577334840fatsLarge molecules, not polymers, Assembled from glycerol and fatty acids, by dehydration reactions. Also called triacylglycerol. Bk
577334841fatty acidA long carbon chain carboxylic acid. vary in length, & number and location of double bonds; three ___ linked to a glycerol molecule form fat.
577334842glycineAn amino acid that functions as a CNS neurotransmitter.
577334843glycogenAn extensively branched glucose storage polysaccharide found in the liver and muscle of animals; the animal equivalent of starch.
577334844glycosidic linkageA covalent bond formed between two monosaccharides by a dehydration reaction.
577334845hemoglobinAn iron-containing protein in red blood cells that reversibly binds oxygen.
577334846hydrogen bondA type of weak chemical bond formed when the slightly positive hydrogen atom of a polar covalent bond in one molecule is attracted to the slightly negative atom of a polar covalent bond in another molecule.
577334847hydrolysisA chemical process that lyses, or splits, molecules by the addition of water; an essential process in digestion.
577334848hydrophobic interactionA type of weak chemical bond formed when molecules that do not mix with water coalesce to exclude the water.
577334849lipidOne of a family of compounds, including fats, phospholipids, and steroids, that are insoluble in water.
577334850macromoleculeA giant molecule formed by the joining of smaller molecules, usually by a condensation reaction. Polysaccharides, proteins, and nucleic acids are macromolecules.
577334851nucleic acidA polymer consisting of many nucleotide monomers; serves as a blueprint for proteins.There are two types.
577334852nucleosideAn organic molecule consisting of a nitrogenous base joined to a five-carbon sugar.
577334853nucleotideThe building block of a nucleic acid, consisting of a five-carbon sugar covalently bonded to a nitrogenous base and a phosphate group.
577334854peptide bondThe covalent bond between two amino acid units, formed by a dehydration reaction.
577334855phospholipidA molecule that is a constituent of the inner bilayer of biological membranes, having a polar, hydrophilic head and a nonpolar, hydrophobic tail.
577334856polynucleotideA polymer consisting of many nucleotide monomers; serves as a blueprint for proteins. There are two types.
577334857polypeptideA polymer of many amino acids linked together by peptide bonds.
577334858polysaccharideA polymer of up to over a thousand monosaccharides, formed by dehydration reactions.
577334859primary structureThe level of protein structure referring to the specific sequence of amino acids.
577334860proteinA three-dimensional biological polymer constructed from a set of 20 different monomers called amino acids.
577334861purineOne of two families of nitrogenous bases found in nucleotides. Adenine (A) and guanine (G) are ___..
577334862pyrimidineOne of two families of nitrogenous bases found in nucleotides. Cytosine (C), thymine (T), and uracil (U) are ___.
577334863quaternary structureThe particular shape of a complex, aggregate protein, defined by the characteristic three-dimensional arrangement of its constituent subunits, each a polypeptide.
577334864ribonucleic acid (RNA)A type of nucleic acid consisting of nucleotide monomers with a ribose sugar and the nitrogenous bases adenine (A), cytosine (C), guanine (G), and uracil (U); usually single-stranded; functions in protein synthesis and as the genome of some viruses.
577334865riboseThe sugar component of RNA.
577334866saturated fatty acidA fatty acid in which all carbons in the hydrocarbon tail are connected by single bonds, thus maximizing the number of hydrogen atoms that can attach to the carbon skeleton.
577334867secondary structureThe localized, repetitive coiling or folding of the polypeptide backbone of a protein due to hydrogen bond formation between peptide linkages.
577334868starchA storage polysaccharide in plants consisting entirely of glucose.
577334869steroidsA type of lipid characterized by a carbon skeleton consisting of four rings with various functional groups attached.
577334870tertiary structureIrregular contortions of a protein molecule due to interactions of side chains involved in hydrophobic interactions, ionic bonds, hydrogen bonds, and disulfide bridges.
577334871triacylglycerolThree fatty acids linked to one glycerol molecule.
577334872unsaturated fatty acidA fatty acid possessing one or more double bonds between the carbons in the hydrocarbon tail which reduces the number of hydrogen atoms attached to the carbon skeleton.
577334873x-ray crystallographyA technique that depends on the diffraction of an ___ beam by the individual atoms of a molecule to study the three-dimensional structure of a molecule.

AP Biology Chapter 9 (Cell Respiration) Flashcards

Quizlet for Mr. McCormick's Test on Cell Respiration on Wednesday 11/14/12.

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506579777cell respirationthe breakdown of food in the cell
506579778aerobic____________ respiration uses oxygen
506579779anaerobic___________ respiration does not use oxygen
506579780CHumans go through: A) Aerobic Respiration B) Anaerobic Respiration C) Both D) Neither
506579781oxidationLoss of electrons (and protons) = ______________
506579782reductionGain of electrons (and protons) = ______________
506579783FalseTrue or False: During oxidation and reduction, electrons are the only subatomic particle being transferred.
506579784oxidizingA compound that receives electrons is called a(n) _____________ agent.
506579785reducingA compound that donates electrons is called a(n) _____________ agent.
506579786electronegativityOxygen is a great oxidizing agent because it has a very high ______________.
506579787electronegativityThe tendency for an atom to attract electrons
50657978838Ultimately, 1 glucose molecule is good for the MAXIMUM production of ____ ATP molecules.
506579789Nicotinahide Adenine DinucleotideNAD = ____________ ____________ _____________
506579790carrierNAD⁺ is an electron (donor / carrier).
506579791highNAD⁺ molecules oftentimes have a very (high / low) level of potential energy.
506579792mitochondriaIf ______________ are present in a cell, then NAD⁺ molecules can aid in the production of a tremendous amount of ATP.
506579793NAD⁺Oxidized NAD = ______
506579794NADHReduced NAD = _______
506579795cytoplasmGlycolysis occurs in the ____________ of a cell.
506579796anaerobicGlycolysis is a(n) _____________ process.
5065797974Gross ATP production of glycolysis = __ ATP
5065797982Net ATP production of glycolysis = ___ ATP
5065797992___ ATP molecules are needed to initiate glycolysis.
506637988CO₂ waterRaw materials for photosynthesis
506637989CO₂ waterMain waste products of respiration
506637990aerobic respirationMost prevalent and efficient catabolic pathway for respiration
506637991exergonicThe breakdown of glucose is (endergonic / exergonic).
506637992redoxReactions that involve electron transfer = ________ reactions
506637993losesAn electron (loses / gains) potential energy when it moves to a more electronegative atom.
506637994hydrogenOrganic molecules with an abundance of __________ generally make great fuels.
506637995FalseTrue or False: Body temperature in humans is high enough to immediately initiate the burning of food
506637996TrueTrue or False: It is most efficient to gradually harness energy from a source rather than harness it quickly
506637997coenzymeNAD⁺ is a ____________.
506637998very littleElectrons lose (very little / a lot) of their potential energy once they are transferred from glucose to NAD⁺.
506637999inner membrane mitochondriaIn eukaryotic cells, the electron transport chain takes place in the _________ ___________ of _____________.
506638000plasma membraneIn prokaryotic cells, the electron transport chain takes place in the ___________ ___________.
506638001Glycolysis Krebs Cycle Oxidative PhosphorylationRespiration is a cumulative function of 3 metabolic stages: _______________ ___________ __________ ____________ __________________
506638002mitochondrial matrixThe citric acid cycle takes place in the _____________ ____________ of eukaryotic cells.
506638003cytosolThe citric acid cycle takes place in the _____________ of prokaryotic cells.
506638004electron transport chain chemiosmosisThe 2 processes that constitute oxidative phosphorylation are: ___________ ___________ __________ _____________
506638005oxidative phosphorylation90% of the ATP produced during respiration happens during ______________ _____________.
506638006substrate level phosphorylationATP produced during glycolysis and the citric acid cycle occur via a mechanism called ____________-__________ ___________.
525000859kinaseType of enzyme involved in phosphorylation
525000860isomeraseType of enzyme involved in structural change
5250008612Pyruvate + 2NADH + 2H⁺ + 4ATPComplete the following equation for glycolysis: C₆H₁₂O₆ + 2ATP + 2NAD⁺ →
525000862FalseTrue or False: After a glucose molecule has undergone glycolysis, the 2 produced pyruvate molecules immediately enter into the Krebs Cycle.
525000863Acetyl CoA + NADH + H⁺ + CO₂Complete the following equation for the acetylation reaction: Pyruvate + NAD⁺ →
5250302631One turn of the Krebs Cycle does work on ___ pyruvate(s).
5250302643 NADH 1 ATP 1 FADH₂ 2 CO₂One turn of the Krebs Cycle produces: ____ _________, _____ _________, ___ _________ (& ____ __________ as a waste product)
525030265matrix mitochondrionThe acetylation reaction occurs in the _________ of the ____________.
525030266oxidized NAD⁺During the electron transport chain NADH is ____________ to form ______.
525030267glucoseThe protons and electrons that reduce NAD⁺ and FAD during respiration originally come from ____________.
525030268protonsDuring the electron transport chain, __________ is/are pumped into the inter-membrane space of the mitochondrion.
525030269decreaseAn increase in the amount of protons within a certain area will (increase / decrease) pH.
525030270increaseA decrease in the amount of protons within a certain area will (increase / decrease) pH.
525030271decreasesAs the electron transport chain progresses, the pH of the inter-membrane space (increases / decreases).
525030272ATP SynthaseEnzyme responsible for the production of ATP during oxidative phosphorylation
525030273oxygenAfter an electron has passed through the final component of the ETC, it will join to the final electron acceptor, __________.
527236278TrueTrue or False: Glycolysis releases less than a quarter of the chemical energy stored in a glucose molecule.
527236279activeAfter pyruvate is produced during glycolysis, it is transferred to the mitochondria via (active / passive) transport.
527236280FalseTrue or False: CO₂ is released as a by-product of glycolysis.
527505368several thousandFor every mitochondrion, there is/are (one / several thousand) electron transport chains.
527505369proteinsMost components of the ETC are _________.
527505370prosthetic groupsNonprotein portions of the ETC crucial in the catalytic functions of certain enzymes
527505371FalseTrue or False: Electron carriers during the ETC are always being oxidized.
528119337ADP inorganic phosphateATP Synthase makes ATP from ______ and ____________ ____________.
528119338chemiosmosisProcess in which energy stored in the form of a hydrogen ion gradient across a membrane is used to drive cellular work such as the synthesis of ATP
528119339statorATP Synthase channel through which H⁺ enters/exits
528119340rotorPart of ATP Synthase; H⁺ enters, part spins as a result of change in shape
5281193411An H⁺ ion goes through ___ full spin(s) in ATP Synthase.
528119342rodPart of ATP Synthase that connects rotor to knob
528119343knobStationary portion of ATP Synthesis that contains sites for ATP production
528119344proton motive forceH⁺ gradient during chemiosmosis is referred to as a _________-_________ _________
5281193453An NADH molecule can ultimately help produce a maximum of ___ ATP molecules.
5281193462An FADH₂ molecule can ultimately help produce a maximum of __ ATP molecules
52814535340Humans put ___% of glucose's chemical potential energy towards ATP Synthesis (more efficient than the 25% cars have w/ relation to gasoline)
535769803respiratoryOther name for electron transport chain (**__________ chain)
535769804TrueTrue or False: In some organisms, oxygen is not the final electron acceptor.
535769805hydrogen sulfide (H₂S)For organisms with a sulfate ion as the final proton acceptor, _________ ____________ is a waste product.
535769806TrueTrue or False: Glycolysis produces a net of 2 ATP in both aerobic and anaerobic conditions.
535769807NADH PyruvateDuring fermentation, _______ becomes oxidized and ___________ becomes reduced.
535769808acetaldehyde ethanolDuring alcohol fermentation, pyruvate becomes _____________ and then is reduced by NADH to form ___________.
535769809directly NADHDuring lactic acid fermentation, pyruvate is reduced (directly / indirectly) by _________.
535769810TrueTrue or False: CO₂ is released during alcohol fermentation.
535769811FalseTrue or False: CO₂ is released during lactic acid fermentation.
535769812liver pyruvateLactate, after fermentation, is sent to the ________ where it is converted to ____________.
535769813Obligate anaerobes___________ ______________ only carry out anaerobic respiration; in fact, they cannot survive in the presence of oxygen.
535769814facultative anaerobesOrganisms that can make enough ATP survive via aerobic or anaerobic pathways are called ____________ _____________.
535769815TrueTrue or False: Some humans cells (e.g. muscle cells) are facultative anaerobes.
535769816TrueTrue or False: Some cell types (e.g. cells of the vertebrae brain) can only undergo aerobic respiration.
535769817TrueTrue or False: Fossils indicate that glycolysis predates aerobic respiration.
539803783cristaOther name for inner membrane of mitochondrion
539803784negativeThe overall free energy change in the electron transport chain is (positive / negative).
5398037854There are ___ main groups in the electron transport chain.
539803786topNADH puts its electrons into the ETC at the very (top / bottom), making for the most potential energy to make ATP.
5398037870How many ATP molecules are produced by fermentation? (Use digit)
539803788middleFADH₂ enters the ETC at the (very top / middle / very bottom).
539803789FalseTrue or False: Component 1 of the ETC is more electronegative than component 3.
539803790electronegativeAs the ETC progresses, each component becomes increasingly ___________.
539803791cyanidePoison that blocks the electron transport chain and therefore ATP production
539803792lactate fermentationIn humans, ___________ ____________ is best described as a way to replenish NAD⁺.
539803793oxygen NAD⁺Fermentation happens during periods of increased physical activity (i.e. exercise) because there is a deficiency in ___________ which creates a deficiency in _________.
539803794TrueTrue or False: It takes ATP to move pyruvate into the mitochondrion.
539803795electronsNAD cannot actually enter into the mitochondrion; only its ____________ enter.
539803796oxygenAs more and more glucose molecules are oxidized (exercise), more _________ is needed.
539803797backup NAD⁺If there is not enough oxygen in a system, a "_________" of NADH molecules is created; a shortage of essential _______ occurs.
539803798fermentationMethod of dealing with NAD⁺ shortage (*______________)
539803799lactate proteinsContrary to popular belief, muscle soreness does not come from __________. It comes from the breakdown of __________ in muscle cells.
539803800FalseTrue or False: As an alternative pathway to oxidative phosphorylation, all organisms go through lactate fermentation.
539803801lactate fermentationSome bacteria go through _________ ___________; the products of this reaction helps make commercial products like yogurt.
539803802alcohol fermentationSimplest / oldest enzymatic reaction studied by man
539803803CO₂ acetaldehydeFirst step in alcohol fermentation: A _____ molecule is cleaved off from pyruvate to produce ______________.
539803804acetaldehyde ethanolNAD is oxidized during alcohol fermentation as ______________ is reduced to become ____________.
539803805water CO₂About 40% of Asians lack enzymes to make _________ / __________ from acetaldehyde and subsequently turn all red when they drink alcohol.
539803806the atmosphere0₂ in the mitochondrion that acts as the final electron acceptor comes from (elsewhere within the cell / the atmosphere).
539803807substrate level phosphorylationEven if the electron transport chain ceases, _____________-___________ ________________ will still exists as a method to make ATP.
539803808TrueTrue or False: Fat is a better electron donor than sugar is.
539803809fasterDuring fermentation, glucose is consumed at a (faster / slower) rate.
539803810facultativeAnaerobes that can survive in the process of oxygen: _____________.
539803811obligateAnaerobes that cannot survive in the presence of oxygen: _____________.
539803812botulismClassic example of obligate anaerobe = _____________ bacteria
5398038133 4__ or __ protons are needed to make one ATP molecule
53980381410One NADH molecule helps to pump about _____ protons into the inter-membrane space.
539803815prosthetic groupNon-protein part of molecule in the ETC: _________ _________
539803816hemoglobinCytochromes (components of the ETC) have metals in them; notably, they are similar to ____________.
539803817produce releasesRespiration does not _________ energy, it _____________ it from stored sources.
539823492electron shuttlesSubatomic particles from NADH move into mitochondria with the help of __________ ___________ that span the mitochondrial membranes.

Chinese Grade 3 Lesson 5 Flashcards

Terms : Hide Images
613148115chēng - to weigh
613148116jiàng - a general
613148117jūn - army / military / arms
613148118cáo - surname
613148119cāo - to hold / to drill / to exercise / to act / to do / to take in hand / to keep / to manage
613148120yùn - to move / to transport / to use / to apply / fortune / luck / fate
613148121shā - to kill / to murder / to slaughter
613148122qiē - to cut / to slice
613148123chōng - to rinse / to collide / to water / to rush / to dash (against) / to wash out / to charge / highway / public road
613148124fù - father
613148125chén - submerge / immerse / sink / deep / profound / to lower / to drop
613148126沿yán - along
613148127àn - bank / shore / beach / coast
613148128jīn - catty / weight equal to 0.5 kg
613148129liáng - to measure

DNA Sequencing Flashcards

Chapter 10 using SIRI

Terms : Hide Images
486345599DNA sequencing is used forDetecting mutations, typing Microorganisms, identifying Human haplotypes and designating polymorphisms
486345600Targeted therapies will be directed atAbnormal DNA sequences
486352352DNA sequencing defineThe order of nucleotides in the DNA molecule
486352353Sanger method DiscoveredDeoxy chain termination sequencing method
486363731Replication factor (RF) In regard to Dideoxy sequencingBacterial virus replicates by infecting E. coli, viral single-stranded circular
486363732Internal labeling DefineFluorescent dye labeled nucleotides, added to the nucleotide sequencing reaction next.
486363733ddNTP DideoxynucleotidesIs used for sequencing ddNTP lack the hydroxyl group found on 3' ribose C of the deoxy nucleotides (dNTP)
486370754Chain termination definedDNA synthesis stop upon incorporation of a ddNTP Into the growing DNA chain
486370755Why does chain termination occur?ddNTP Does not have a hydroxyl group at the 3' sugar carbon, so the 5'-3' phosphodiester Bond cannot occur & establish subsequent nucleotide (Create chain)
486374165Sections of DNA Created from PCR are often used as sequencing templates QAPCR needs to be free of residual components such as primers and nucleotides
486376108QA:: PCR Amplicon's (Replicated DNA Sections) can be cleaned by ___, ___, ___.Alcohol precipitation, enzymatic digestion with alkaline phosphatase, or if urines and washing on solid phase matrix (Bead or column)
486380311QA Running PCR Amplicon's on and agarose gel confirms the product being sequence and ____Cleans the template
486380312Components required for DNA synthesis areTemplate, primer, enzyme, buffers, dNTPS. All four ingredients go in four separate tubes each two has individual ddATP, ddCTP, ddGTP, ddTTP
486407793ddNTP Concentrations are critical for generation of a readable sequence because1. high levels= polymerization will terminate too frequently Early along the template 2. Low levels= Infrequent or no termination will occur
486416707Stop buffer consists of20 mM EDTA To Chile cations and stop enzyme activity, formamide to denature the products of the synthesis reaction, and gel loading dies (bromiphenol blue and or xylene cyanol)
486416708QA Maintaining consistent reaction times will provideConsistent band intensity
486416709Synthesize fragments are loaded onto a denaturing poly Accra Madlib gel, in ____ lanesfour adjacent lanes
486421275Sequencing latter defineThe four-lane gel electrophoresis pattern of the products of the four sequencing reactions
486421276The sequencing ladder Deduces DNA sequences, showing smallest and largest Fragments in which locationSmallest fragments, fastest migrating, terminate closest to the primer, bottom of the gel Larger fragments found near 3', Near well
486424937Sequencing latter (polyacrymalide gel plate) is read fromBottom to top, Smallest to largest Fragments of DNA, 5-3', Towards Wells where dNTP'S (A,T,C,G) were originally placed
486427633Cycle sequencing defineUse of thermal cyclers for the sequencing reaction using heat-stable enzymes such as Therminator and Thermosequenase
486650718Automated Fluorescent sequencing vs manual sequencingSame chemistry, Using double-stranded templates and cycle sequencing. Automated cycle sequencing does Not require additional regions to start and stop the reaction therefore is easily adaptable to high throughput applications and automation
486650719QA: electrophoresis and reading of sequential ladder can be automated, ____ is required for reading the DNA sequence ladder.Fluorescent dyes (instead of radioactive nucleotides to label the primers or sequencing fragments)
486660818Automated fluorescent sequencing, Using capillary or single Gelles Lane Is spread byEach nucleotide (Reaction mix) Is read by It's own COLOR (A, T, C, G have a Different color each)
486660819Automated sequencing: dye primer vs dye terminatorDye primer -5' END- uses labeled primers, the products of all four reactions are resolved together in one lane of gel or any capillary. Dye terminators - 3' END- One reaction to this necessary since they will be distinguished directly by the ddNTP on their 3' end
486666265After sequencing reaction using fluorescent dye terminators, excess dye terminators must be removed from the sequencing ladder or cleaned by ____ or _____ or _____.Columns or beads or ethanol precipitation
486666266Sequencing ladder preparation protocolAddition of my terminators to single file, sequencing cleaned, ladder completely denatured,
486670149Fluorescent detection equipment yields results as an _____. Software assigns one of four arbitrary colorsElectropherogram (Rather than a gel pattern) Passed by laserbeam, Beam excites fragment, dye emits it's florescents and is captured by the detector.
486674004Quality of electropherogram depends on what three things1 Quality of template, 2 Efficiency the sequencing reaction, 3The cleanliness of the sequencing latter
486674005Dye blobs ( bright flashes of fluorescence) - Are caused by?Tell your to clean the sequencing ladder properly, They obliterate parts of the sequencing read
486676814QA Sequencing Both strands of DNA is important toConfirm sequence data. This is critical for confirmation mutations or polymorphisms In a sequence
486676815Heterozygous mutations appear asTwo peaks of different color directly on top one another
486676816Heterozygous deletions or insertions appear as(ex. BRCA Frameshift mutations) affect All positions of the sequence downstream of the mutation and are more easily detected
486676817Somatic mutationsI'm more difficult to detect they may be debited by normal sequences that mask the somatic changes
486682469Pyrosequencing DNA sequencing Does not have to make aSequencing ladder
486682470Bisulfate DNA sequencing or Methylation specific sequencingMethylation is detected by comparing the treated sequence with an untreated sequence and noting where the sequence C/T base pairs are not changed to U/G

Biotech - PCR (DNA Sequencing, slides 1-10) Flashcards

Terms : Hide Images
319064880because DNA encodes for all of the genes in an organism, the ___ of DNA is very importantsequence
319064881DNA is sequenced by essentially ____ DNA in the presence of special nucleotides which result in ____ of DNAreplicating, fragments
319064882sequencing technology relies upon the fact that ___ ___ needs a templateDNA polymerase
319064883sequencing technology relies upon the fact that DNA polymerase needs a ___template
319064884DNA is copied by reading the template strand and predicting the newly synthesized strand using __ __ rulesbase pair
319064885T/F: DNA replication requires a templatetrue
319070828the key component in sequencing is the incorporation of a special nucleotide called a ____ nucleotidedideoxy
319070829dideoxy nucleotides do not have a ____ so they are incapable of forming nucleic acids3'OH
319070830what has no 2'OH or 3'OH?dideoxy nucleotide
319070831dideoxy nucleotides can only be added at the ___ (beginning/end) of a DNA strandend
319070832dideoxy nucleotides (ddNTP) fall into that class of __ __ b/c once they are incorporated they halt synthesischain terminators
319070833if ddNTP is included, ___ of DNA are generated, each "capped" with a ddNTPfragments
319070834what is the specific ddNTP determined by?template strand
319070835what happens? ddNTP is added in place of ONE of the nucleotides (ex. ATP, CTP, ddGTP)synthesis stops
319070836slides 4-6 show good representations of dideoxynucleotides...
319070837what happens? a small amount of ddNTP is added and ALL FOUR dNTPs are present? (ex. ATP, CTP, GTP, TTP, ddGTP)two DNA fragments are produced (b/c either GTP or ddGTP can be incorporated)
319070838to determine how many DNA fragments there are you can use ____electrophoresis
319070839T/F: electrophoresis separates DNA fragments based on sizetrue
319074739smaller pieces of DNA will show up at the ____ (bottom/top) of the electrophoresis gelbottom (b/c they can move farther)
319074740____ (smaller/larger) pieces of DNA will show up at the top of the electrophoresis gellarger
319074741if you have ATP, CTP, GTP, TTP, and a small amount of ddGTP, what will every fragment end in? what is the exception?every fragment ends in G, exception is the full-length piece
319074742the size of the DNA fragment is based upon the number of ___ ___base pairs
319074743slide 8 is a good one for a visual of electrophoresis...
319074744to determine the sequence by looking at electrophoresis gel, you start at the ____ of the gel and work your way ___bottom, up
319074745the bottom of the electrophoresis gel represents the ___ end and the top of the gel is the ___ end5', 3'
319074746with electrophoresis, we are determining the sequence of the ___ strandcomplement
319074747T/F: when using electrophoresis gel, the sequence of the template strand is determinedfalse (complement)
319074748T/F: when using electrophoresis gel, the sequences of the complement strand is determinedtrue
323719274does the primer show up on electrophoresis gel in DNA sequencing?no (the gel shows the band that corresponds to the last nucleotide)
323719275T/F: the primer should be complementary to the sequence of template strandtrue
323719276in DNA sequencing, the electrophoresis gel shows the _____ strand of DNAcomplement
323719277at the bottom of the gel of DNA sequencing the first nucleotide that shows up is next to the ____primer

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