Solutions to Molecular Biology Unit Exam Question 1 Consider the following origin of replication that is found on a chromosome. The sequence of region 1 is shown below. Region 1: 5??CTGACTGACA?3? 3??GACTGACTGT?5? 5? 3? 5? 3? Region 1 ori top ? bottom ? a) Within Region 1, which strand will be the template for leading strand synthesis, the top or the bottom? The bottom strand will be the template for leading strand synthesis. b) If we assume that a lagging strand fragment is made from region 1, what will be its sequence? 5? T G T C A G T C A G 3? c) You examine DNA replication in an E. coli mutant, which has a partially defective DNA polymerase. In vitro
exam2 soln
Solutions to Molecular Biology Unit Exam Question 1 Consider the following origin of replication that is found on a chromosome. The sequence of region 1 is shown below. Region 1: 5??CTGACTGACA?3? 3??GACTGACTGT?5? 5? 3? 5? 3? Region 1 ori top ? bottom ? a) Within Region 1, which strand will be the template for leading strand synthesis, the top or the bottom? The bottom strand will be the template for leading strand synthesis. b) If we assume that a lagging strand fragment is made from region 1, what will be its sequence? 5? T G T C A G T C A G 3? c) You examine DNA replication in an E. coli mutant, which has a partially defective DNA polymerase. In vitro
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