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AP Biology: Biotechnology Flashcards

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8075015225Recombinant DNADNA produced by combining DNA from different sources0
8075015226BiotechnologyA form of technology that uses living organisms, usually genes, to modify products, to make or modify plants and animals, or to develop other microorganisms for specific purposes.1
8075015227PlasmidsSmall rings of DNA found naturally in some bacterial cells in addition to the main bacterial chromosome. Can contain genes for antibiotic resistance, or other "contingency" functions.2
8075015228Gene CloningThe production of multiple copies of a gene.3
8075015229Restriction enzymesEnzyme that cuts DNA at a specific sequence of nucleotides4
8075015230Sticky endA single-stranded end of a double-stranded DNA restriction fragment.5
8075015231DNA LigaseA linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain.6
8075015232Cloning vectorDNA molecules that can carry foreign DNA into a host cell and replicate there.7
8075015233Complimentary DNA (cDNA)is made from mRNA by reverse transcriptase8
8075015234polymerase chain reaction (PCR)A method of producing thousands of copies of DNA segment using the enzyme DNA polymerase9
8075015235gel electrophoresisProcedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel10
8075015236southern blottingA hybridization technique that enables researchers to determine the presence of certain nucleotide sequences in a sample of DNA.11
8075015237reverse transcriptase- PCRAn enzyme encoded by some certain viruses (retroviruses) that uses RNA as a template for DNA synthesis.12
8075015238Genetic profileAn individual's unique set of genetic markers, detected most often today by PCR or, previously, by electrophoresis and nucleic acid probes.13
8075016688TransposonA sequence of DNA that can move about the genome.14
8075035551Gene familiesGroups of closely related genes15
8075041540PseudogenesResult from mutations that cause a loss of function rather than an enhanced or new function.16

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