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AP Biology: Chapter 18 Flashcards

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6286755897Feedback Inhibition- The product acts as an allosteric inhibitor of the 1st enzyme in tryptophan pathway but this is wasteful production of enzymes0
6286755898Gene Regulation- Don't block the enzyme's function, block transcription of genes for all enzymes in tryptophan pathway - Saves energy by not wasting it on unnecessary protein synthesis1
6286755899Operon- Genes grouped together with related functions2
6286755900Promoter- RNA polymerase binding site - Controls transcription of all genes in the operon - Transcribed as one unit and a single mRNA is made3
6286755901Operator- DNA binding site of repressor proteins4
6286755902Repressor Operon- Binds to DNA at the operator site - Blocking RNA polymerase - Blocks transcription - Similar to how a skateboard stops on a curb - ANABOLIC pathways (build) - Synthesizing end products - Cell allocates excess resources to other uses5
6286755903Inducible Operon- CATABOLIC pathways (destroy) - Digesting nutrients to simpler molecules - Produce enzymes only when nutrients are available - Cell avoids making proteins that have nothing to do, allocates resources6
6286755904DNA Packing- DNA coiling and folding - Degree of packing regulates transcription - No transcription, genes off - Wrapped histones - Double helix - Nucleosomes (beads on a string) - Chromatin fiber - Looped domains - Chromosome7
6286755905DNA Methylation- Blocks transcription factors and genes turned off - Attachment of methyl groups to cytosine - Nearly permanent inactivation of genes - Ex: Barr body8
6286755906Acetylation of Histones- Unwinds DNA - Loose histone enables transcription and genes to turn on - Attachment of COCH3 - Conformational change to histone proteins - Transcription factors have easier access to genes9
6286755907Transcription Initiation- Controls regions of DNA - Promoter closely controls DNA sequence and binding of RNA polymerase/ transcription factors (base rate) - Enhancer distantly controls DNA sequences and binds to activator proteins (high rate)10
6286755908Post Transcriptional Control- Alternative RNA splicing - Variable processing of exons creates a family of proteins11
6286755909Regulation of mRNA Degradation- Life span of mRNA determines amount of protein synthesis12
6286755910RNA Interference siRNA- Short segments of RNA - Binds to mRNA - Creates sections of double stranded mRNA - Death tag for mRNA (Degration) - Causes gene silencing - Post transcriptional control - Turns off genes because no proteins are produced13
6286755911Control of Translation- Blocks initiation of translation stage - Regulatory protein attaches to the 5 end of mRNA - Prevents the attachment of ribosomal subunits and initiator tRNA - Blocks translation of mRNA protein14
6286755912Protein Processing and Degration- Folding, cleaving, adding sugar groups, and targets for transport - Ubiquitin tagging and proteasome degradation15
6286755913Ubiquitin- Death Tag - Marks unwanted proteins with a label - 76 amino acid polypeptide - Labeled proteins are broken down rapidly in waste disposers/proteasomes16
6286755914Proteasome- Protein Degrading Machine - Waste disposer - Breaks down any proteins into 7-9 amino acid fragments - Cellular recycling17
6286755915A mutation that makes the regulatory gene of an inducible operon nonfunctional would result in- continuous transcription of the operon's genes.18
6286755916A mutation that inactivates the regulator gene of a repressible operon in an E. coli cell would result in- continuous transcription of the structural gene controlled by that regulator.19
6286755917The expression of genes can be controlled at all the following stages of protein synthesis EXCEPT:- protein folding20
6286755918All of the following are post-transcriptional modifications of eukaryotic mRNA EXCEPT:- Peptide bonds are formed21

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