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AP BIology DNA Replication Flashcards

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9350084864DNA Replicationthe process by which a DNA molecule is copied; also called DNA synthesis0
9350084865Transformationa change in genotype and phenotype due to the assimilation of external DNA by a cell transforming substance- DNA1
9350084866BacteriophagesViruses that infect bacteria Bacteria eaters2
9350084867Virusinfects a cell and takes over the cell's metabolic machinery3
9350084868Hershey and ChaseConcluded that phage DNA entered bacterial host cells, but phage proteins did not, so DNA functions as the genetic material4
9350084869GriffithConcluded that nonpathogenic bacteria transformed into pathogenic bacteria by an unknown, heritable substance from the dead S cells that enabled the R cells to make capsules5
9350084870Chargaff's Lawthe base compostion of DNA varies between species and for each species, the percentages of A and T bases are roughly equal to the percentages of the G and C bases6
9350084871Rosalind Franklinaccomplished X-ray crystallographer that discovered the double helix of DNA7
9350084872Watson and CrickCame up with the structural model of DNA8
9350084873Double helixtwo strands9
9350084874Antiparallelsubunits run in opposite directions10
9350084875Nitrogenous bases of DnaA, T, C, G11
9350084876PurinesA and G Nitrogenous bases with two organic rings12
9350084877PyrimidinesC and T Nitrogenous base with a single organic ring13
9350084878Conservative modeltwo parental strands reassociate after acting as templates for new strands thus restoring the parental double helix14
9350084879Semiconservative Modelthe two strands of the parental molecule separate and each functions as a template for synthesis of a new complementary strand- most common15
9350084880Origins of Replicationshort stretches of DNA having a specific sequence of nucleotides16
9350084881Replication Forka Y shaped region where the parental strands of DNA are being unwound17
9350084882Helicasesenzymes that untwist the double helix at the replication forks, separating the two parental strands and make them available as template strands18
9350084883Single Strand Binding ProteinsBind to the unpaired DNA strands keeping them from repairing19
9350084884TopoisomeraseThe untwisting of double helix causes tighter twisting and strain ahead of replication fork Relieve this strain by breaking swiveling, and rejoining DNA strands20
9350084885PrimerThe initial nucleotide chain that is produced during DNA synthesis is actually a short stretch of RNA21
9350084886PrimaseSynthesizes the primer Starts a complementary RNA chain from a single RNA nucleotide, adding more RNA nucleotides one at a time, using the parental DNA strand as a template22
9350084887DNA polymerasesEnzyme that catalyze the synthesis of new DNA by adding nucleotides to a preexisting chain23
9350084888Leading strandStrand that continuously adds nucleotides to the new complementary strand as the fork progresses DNA pol III24
9350084889Lagging StrandThe strand that DNA pol III works away from the replication fork Synthesized discontinuosly as a series of segments25
9350084890Okazaki fragmentsSeries of segments that are 1000-2000 nucleotides long26
9350084891DNA Ligasejoins the sugar phosphate backbones of all the Okazaki fragments into a continuous DNA strand27
9350084892DNA pol IIISynthesizes new DNA strands by adding nucleotides to an RNA primer or a pre-existing DNA strand28
9350084893DNA pol IRemoves RNA nucleotides of primer from 5' end and replaces them with DNA nucleotides29
9350084894Mismatch repairOther enzymes remove and replace incorrectly paired nucleotides that have resulted from replication errors30
9350084895NucleaseDNA cutting enzyme that cuts out the damaged parts of the strand and fills the space with nucleotides using the undamaged strand as a template31
9350084896Nucleotide excision repairDNA repair system where teams of enzymes detect and repair the DNA, the nuclease cuts out the damaged DNA and removes it, fills in the missing nucleotides and the DNA ligase seals the free end of the new DNA to the old DNA making the strand complete32
9350084897TelomeresSpecial nucleotide sequences at the ends of chromosomes TTAGGG is repeated between 100-1000 times Prevent the staggered ends of daughter molecule from activitating cell's system for monitoring DNA damage33
9350084898TelomeraseEnzyme that catalyzes the lengthening of telomeres in eukaryotic germ cells and restores the original length and compensating for the shortening that occurs during DNA replication34
9350084899HistonesProteins that are responsible for the first level of DNA packing in chromatin35
9350084900Nucleosomethe basic unit of DNA packing36
9350084901Chromatincomplex of DNA and protein37

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