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AP BIology DNA Replication Flashcards

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9481660517DNA Replicationthe process by which a DNA molecule is copied; also called DNA synthesis0
9481660518Transformationa change in genotype and phenotype due to the assimilation of external DNA by a cell transforming substance- DNA1
9481660519BacteriophagesViruses that infect bacteria Bacteria eaters2
9481660520Virusinfects a cell and takes over the cell's metabolic machinery3
9481660521Hershey and ChaseConcluded that phage DNA entered bacterial host cells, but phage proteins did not, so DNA functions as the genetic material4
9481660522GriffithConcluded that nonpathogenic bacteria transformed into pathogenic bacteria by an unknown, heritable substance from the dead S cells that enabled the R cells to make capsules5
9481660523Chargaff's Lawthe base compostion of DNA varies between species and for each species, the percentages of A and T bases are roughly equal to the percentages of the G and C bases6
9481660524Rosalind Franklinaccomplished X-ray crystallographer that discovered the double helix of DNA7
9481660525Watson and CrickCame up with the structural model of DNA8
9481660526Double helixtwo strands of antiparellel nucleotides9
9481660527Antiparallelsubunits run in opposite directions10
9481660528Nitrogenous bases of DnaA, T, C, G11
9481660529PurinesA and G Nitrogenous bases with two organic rings12
9481660530PyrimidinesC and T Nitrogenous base with a single organic ring13
9481660531Conservative modeltwo parental strands reassociate after acting as templates for new strands thus restoring the parental double helix14
9481660532Semiconservative Modelthe two strands of the parental molecule separate and each functions as a template for synthesis of a new complementary strand- most common15
9481660533Origins of Replicationshort stretches of DNA having a specific sequence of nucleotides16
9481660534Replication Forka Y shaped region where the parental strands of DNA are being unwound17
9481660535Helicasesenzymes that untwist the double helix at the replication forks, separating the two parental strands and make them available as template strands18
9481660536Single Strand Binding ProteinsBind to the unpaired DNA strands keeping them from repairing19
9481660537TopoisomeraseThe untwisting of double helix causes tighter twisting and strain ahead of replication fork Relieve this strain by breaking swiveling, and rejoining DNA strands20
9481660538PrimerThe initial nucleotide chain that is produced during DNA synthesis is actually a short stretch of RNA21
9481660539PrimaseSynthesizes the primer Starts a complementary RNA chain from a single RNA nucleotide, adding more RNA nucleotides one at a time, using the parental DNA strand as a template22
9481660540DNA polymerasesEnzyme that catalyze the synthesis of new DNA by adding nucleotides to a preexisting chain23
9481660541Leading strandStrand that continuously adds nucleotides to the new complementary strand as the fork progresses DNA pol III24
9481660542Lagging StrandThe strand that DNA pol III works away from the replication fork Synthesized discontinuosly as a series of segments25
9481660543Okazaki fragmentsSeries of segments that are 1000-2000 nucleotides long26
9481660544DNA Ligasejoins the sugar phosphate backbones of all the Okazaki fragments into a continuous DNA strand27
9481660545DNA pol IIISynthesizes new DNA strands by adding nucleotides to an RNA primer or a pre-existing DNA strand28
9481660546DNA pol IRemoves RNA nucleotides of primer from 5' end and replaces them with DNA nucleotides29
9481660547Mismatch repairOther enzymes remove and replace incorrectly paired nucleotides that have resulted from replication errors30
9481660548NucleaseDNA cutting enzyme that cuts out the damaged parts of the strand and fills the space with nucleotides using the undamaged strand as a template31
9481660549Nucleotide excision repairDNA repair system where teams of enzymes detect and repair the DNA, the nuclease cuts out the damaged DNA and removes it, fills in the missing nucleotides and the DNA ligase seals the free end of the new DNA to the old DNA making the strand complete32
9481660550TelomeresSpecial nucleotide sequences at the ends of chromosomes TTAGGG is repeated between 100-1000 times Prevent the staggered ends of daughter molecule from activitating cell's system for monitoring DNA damage33
9481660551TelomeraseEnzyme that catalyzes the lengthening of telomeres in eukaryotic germ cells and restores the original length and compensating for the shortening that occurs during DNA replication34
9481660552HistonesProteins that are responsible for the first level of DNA packing in chromatin35
9481660553Nucleosomethe basic unit of DNA packing36
9481660554Chromatincomplex of DNA and protein37

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