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AP BIology DNA Replication Flashcards

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5638978472DNA Replicationthe process by which a DNA molecule is copied; also called DNA synthesis0
5638978473Transformationa change in genotype and phenotype due to the assimilation of external DNA by a cell transforming substance- DNA1
5638978474BacteriophagesViruses that infect bacteria Bacteria eaters2
5638978475Virusinfects a cell and takes over the cell's metabolic machinery3
5638978476Hershey and ChaseConcluded that phage DNA entered bacterial host cells, but phage proteins did not, so DNA functions as the genetic material4
5638978477GriffithConcluded that nonpathogenic bacteria transformed into pathogenic bacteria by an unknown, heritable substance from the dead S cells that enabled the R cells to make capsules5
5638978478Chargaff's Lawthe base compostion of DNA varies between species and for each species, the percentages of A and T bases are roughly equal to the percentages of the G and C bases6
5638978479Rosalind Franklinaccomplished X-ray crystallographer that discovered the double helix of DNA7
5638978480Watson and CrickCame up with the structural model of DNA8
5638978481Double helixtwo strands9
5638978482Antiparallelsubunits run in opposite directions10
5638978483Nitrogenous bases of DnaA, T, C, G11
5638978484PurinesA and G Nitrogenous bases with two organic rings12
5638978485PyrimidinesC and T Nitrogenous base with a single organic ring13
5638978486Conservative modeltwo parental strands reassociate after acting as templates for new strands thus restoring the parental double helix14
5638978487Semiconservative Modelthe two strands of the parental molecule separate and each functions as a template for synthesis of a new complementary strand- most common15
5638978488Dispersive Modeleach strand of both daughter molecules contains a mixture of old and newly synthesized DNA16
5638978489Origins of Replicationshort stretches of DNA having a specific sequence of nucleotides17
5638978490Replication Forka Y shaped region where the parental strands of DNA are being unwound18
5638978491Helicasesenzymes that untwist the double helix at the replication forks, separating the two parental strands and make them available as template strands19
5638978492Single Strand Binding ProteinsBind to the unpaired DNA strands keeping them from repairing20
5638978493TopoisomeraseThe untwisting of double helix causes tighter twisting and strain ahead of replication fork Relieve this strain by breaking swiveling, and rejoining DNA strands21
5638978494PrimerThe initial nucleotide chain that is produced during DNA synthesis is actually a short stretch of RNA22
5638978495PrimaseSynthesizes the primer Starts a complementary RNA chain from a single RNA nucleotide, adding more RNA nucleotides one at a time, using the parental DNA strand as a template23
5638978496DNA polymerasesEnzyme that catalyze the synthesis of new DNA by adding nucleotides to a preexisting chain24
5638978497Leading strandStrand that continuously adds nucleotides to the new complementary strand as the fork progresses DNA pol III25
5638978498Lagging StrandThe strand that DNA pol III works away from the replication fork Synthesized discontinuosly as a series of segments26
5638978499Okazaki fragmentsSeries of segments that are 1000-2000 nucleotides long27
5638978500DNA Ligasejoins the sugar phosphate backbones of all the Okazaki fragments into a continuous DNA strand28
5638978501DNA pol IIISynthesizes new DNA strands by adding nucleotides to an RNA primer or a pre-existing DNA strand29
5638978502DNA pol IRemoves RNA nucleotides of primer from 5' end and replaces them with DNA nucleotides30
5638978503Mismatch repairOther enzymes remove and replace incorrectly paired nucleotides that have resulted from replication errors31
5638978504NucleaseDNA cutting enzyme that cuts out the damaged parts of the strand and fills the space with nucleotides using the undamaged strand as a template32
5638978505Nucleotide excision repairDNA repair system where teams of enzymes detect and repair the DNA, the nuclease cuts out the damaged DNA and removes it, fills in the missing nucleotides and the DNA ligase seals the free end of the new DNA to the old DNA making the strand complete33
5638978506TelomeresSpecial nucleotide sequences at the ends of chromosomes TTAGGG is repeated between 100-1000 times Prevent the staggered ends of daughter molecule from activitating cell's system for monitoring DNA damage34
5638978507TelomeraseEnzyme that catalyzes the lengthening of telomeres in eukaryotic germ cells and restores the original length and compensating for the shortening that occurs during DNA replication35
5638978508HistonesProteins that are responsible for the first level of DNA packing in chromatin36
5638978509Nucleosomethe basic unit of DNA packing37
5638978510Chromatincomplex of DNA and protein38
5638978511Heterochromatincentromeres and telomeres exist in a highly condensed state with visible irregular clumps making it largely inaccessible39
5638978512Euchromatincentromeres and telomeres exist in a less compacted state that is very accessible40

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