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AP BIology DNA Replication Flashcards

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6215151439DNA Replicationthe process by which a DNA molecule is copied; also called DNA synthesis0
6215151440Transformationa change in genotype and phenotype due to the assimilation of external DNA by a cell transforming substance- DNA1
6215151441BacteriophagesViruses that infect bacteria Bacteria eaters2
6215151442Virusinfects a cell and takes over the cell's metabolic machinery3
6215151443Hershey and ChaseConcluded that phage DNA entered bacterial host cells, but phage proteins did not, so DNA functions as the genetic material4
6215151444GriffithConcluded that nonpathogenic bacteria transformed into pathogenic bacteria by an unknown, heritable substance from the dead S cells that enabled the R cells to make capsules5
6215151445Chargaff's Lawthe base compostion of DNA varies between species and for each species, the percentages of A and T bases are roughly equal to the percentages of the G and C bases6
6215151446Rosalind Franklinaccomplished X-ray crystallographer that discovered the double helix of DNA7
6215151447Watson and CrickCame up with the structural model of DNA8
6215151448Double helixtwo strands9
6215151449Antiparallelsubunits run in opposite directions10
6215151450Nitrogenous bases of DnaA, T, C, G11
6215151451PurinesA and G Nitrogenous bases with two organic rings12
6215151452PyrimidinesC and T Nitrogenous base with a single organic ring13
6215151453Conservative modeltwo parental strands reassociate after acting as templates for new strands thus restoring the parental double helix14
6215151454Semiconservative Modelthe two strands of the parental molecule separate and each functions as a template for synthesis of a new complementary strand- most common15
6215151456Origins of Replicationshort stretches of DNA having a specific sequence of nucleotides16
6215151457Replication Forka Y shaped region where the parental strands of DNA are being unwound17
6215151458Helicasesenzymes that untwist the double helix at the replication forks, separating the two parental strands and make them available as template strands18
6215151459Single Strand Binding ProteinsBind to the unpaired DNA strands keeping them from repairing19
6215151460TopoisomeraseThe untwisting of double helix causes tighter twisting and strain ahead of replication fork Relieve this strain by breaking swiveling, and rejoining DNA strands20
6215151461PrimerThe initial nucleotide chain that is produced during DNA synthesis is actually a short stretch of RNA21
6215151462PrimaseSynthesizes the primer Starts a complementary RNA chain from a single RNA nucleotide, adding more RNA nucleotides one at a time, using the parental DNA strand as a template22
6215151463DNA polymerasesEnzyme that catalyze the synthesis of new DNA by adding nucleotides to a preexisting chain23
6215151464Leading strandStrand that continuously adds nucleotides to the new complementary strand as the fork progresses DNA pol III24
6215151465Lagging StrandThe strand that DNA pol III works away from the replication fork Synthesized discontinuosly as a series of segments25
6215151466Okazaki fragmentsSeries of segments that are 1000-2000 nucleotides long26
6215151467DNA Ligasejoins the sugar phosphate backbones of all the Okazaki fragments into a continuous DNA strand27
6215151468DNA pol IIISynthesizes new DNA strands by adding nucleotides to an RNA primer or a pre-existing DNA strand28
6215151469DNA pol IRemoves RNA nucleotides of primer from 5' end and replaces them with DNA nucleotides29
6215151470Mismatch repairOther enzymes remove and replace incorrectly paired nucleotides that have resulted from replication errors30
6215151471NucleaseDNA cutting enzyme that cuts out the damaged parts of the strand and fills the space with nucleotides using the undamaged strand as a template31
6215151472Nucleotide excision repairDNA repair system where teams of enzymes detect and repair the DNA, the nuclease cuts out the damaged DNA and removes it, fills in the missing nucleotides and the DNA ligase seals the free end of the new DNA to the old DNA making the strand complete32
6215151473TelomeresSpecial nucleotide sequences at the ends of chromosomes TTAGGG is repeated between 100-1000 times Prevent the staggered ends of daughter molecule from activitating cell's system for monitoring DNA damage33
6215151474TelomeraseEnzyme that catalyzes the lengthening of telomeres in eukaryotic germ cells and restores the original length and compensating for the shortening that occurs during DNA replication34
6215151475HistonesProteins that are responsible for the first level of DNA packing in chromatin35
6215151476Nucleosomethe basic unit of DNA packing36
6215151477Chromatincomplex of DNA and protein37

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