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AP Biology Molecular Genetics Flashcards

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8090667830DNA ligaseA linking enzyme essential for DNA replication and recombinant DNA techniques0
8090667834Histonesproteins around which eukaryotic DNA is tightly coiled in chromatin1
8090667837TranscriptionThe synthesis of RNA using DNA as a template (takes place in the nucleus)2
8090667838Messenger RNA(produced during transcription) It carries the genetic message of DNA to the protein-making machinery of the cell in the cytoplasm, the ribosome3
8090667841RNA PolymeraseAn enzyme that separates the two DNA strands and connects the RNA nucleotides as they base-pair along the DNA template strand (can only add RNA nucleotides to the 3' end of strand, so RNA elongates in the 5' to 3' direction4
8090667842PromoterSpecific region of a gene where RNA polymerase can bind and begin transcription5
8090667843TerminatorThe DNA sequence that signals the end of transcription6
8090667845Transcription FactorsAssist the bind of RNA polymerase to the promoter and thus the initiation of transcription7
8090667851IntronsNoncoding segments of nucleic acid that lie between coding sequences.8
8090667852Exonsexpressed sequence of DNA; codes for a protein9
8090667853snRNAFound only in the nucleus of eukaryotes and functions to remove introns from mRNA10
8090667854RibozymeRNA enzyme that removes introns and splices exons together11
8090667869Operonset of prokaryotic operator and promoter sites and the structural genes they control12
8090667870Regulatory GenesProduce repressor proteins that bind to the operatore site (when regulatory protein occupies operator site RNA polymerase is blocked and operon is off)13
8090667871Repressible OperonNormally on but can be inhibited; repressor protein produced by the regulatory gene is inactive14
8090667872Inducible OperonAn operon that is normally off but can be activated and is normally catabolic15
8090667873DNA methylationThe addition of methyl groups to DNA which causes DNA to be more tightly packaged, reducing gene expression16
8090667874Histone AcetylationAcetylation of histone "loosens" DNA so that it can be transcribed.17
8090667883Recombinant DNADNA that has been artificially made using DNA from different sources18
8090667884Gene cloningThe process by which scientists can produce multiple copies of specific segments of DNA that they can work with in the lab19
8090667885Restriction EnzymesUsed to cut strands of DNA at specific locations20
8090667886Sticky endThe single stranded end of DNA when restriction enzymes cut it (can form hydrogen bonds with complementary single-stranded pieces of DNA) DNA ligase(an enzyme) can seal those two single-stranded pieces21
8090667890Prokaryotic DNA traitsno introns, not in nucleus, circular, one chromosome, plasmids22
8090667891Eukaryotic DNA traitshistones, introns, linear, more than one chromosome, chromatin23
8090721227electrophoresistechnique used in laboratories in order to separate DNA/RNA, proteins based on size24
8090749271plasmidBacterial genes outside the main chromosome25
8090752515transformationgenetic alteration of a cell resulting from the direct uptake of genetic material from its surroundings through the cell membrane26
8090758926micro RNAa RNA fragment that prevents the production of a particular protein by binding to and destroying the messenger RNA that would have produced the protein.27
8090765055RNAiRNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules28
8090771700cytoplasmic determinantsregions of the cytoplasm accumulate chemicals whose distribution is heterogenic. They play a role in development of the embryo's organs. Each type of cell is determined by a particular determinant29
8090784202pattern formationgeneration of cell fates in space and time controlled by genes. Morphogenesis.30
8090796251PCRtechnique used to amplify DNA generating millions of copies of a particular DNA sequence31
8090805793hox genea group of genes that control the body plan of an embryo along the head-tail axis. Hox proteins determine the type of appendages or vertebrae that will form on a segment32
8090819811transposona DNA sequence that can change position within a genome, sometimes creating or reversing mutations and altering the cell's genetic identity and genome size.33
8090834586p53 genehalts the cell cycle when the cell is need of repair.34
8154857407reverse transcriptaseAn enzyme used by retroviruses that catalyzes the synthesis of DNA on an RNA template.35
8154860420cDNAComplementary strand of DNA synthesized from an RNA template by the enzyme reverse transcriptase.36
8154962269inductionThe process by which neighboring cells can influence the determination of a cell.37
8154968001inducerA specific small molecule that inactivates the repressor in an operon.38
8154971606activatorA protein that binds to DNA and stimulates transcription of a specific gene.39
8154977147transductionTransfer of genes between bacteria by bacteriophages.40

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