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AP Biology Review Chapter 20 Flashcards

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6769313397Recombinant DNADNA produced by combining DNA from different sources0
6769313398BiotechnologyA form of technology that uses living organisms, usually genes, to modify products, to make or modify plants and animals, or to develop other microorganisms for specific purposes.1
6769313399PlasmidsSmall rings of DNA found naturally in some bacterial cells in addition to the main bacterial chromosome. Can contain genes for antibiotic resistance, or other "contingency" functions.2
6769313400Gene CloningThe production of multiple copies of a gene.3
6769313401Restriction enzymesEnzyme that cuts DNA at a specific sequence of nucleotides4
6769313402Sticky endA single-stranded end of a double-stranded DNA restriction fragment.5
6769313403DNA LigaseA linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain.6
6769313404Cloning vectorDNA molecules that can carry foreign DNA into a host cell and replicate there.7
6769313405Complimentary DNA (cDNA)is made from mRNA by reverse transcriptase8
6769313406polymerase chain reaction (PCR)A method of producing thousands of copies of DNA segment using the enzyme DNA polymerase9
6769313407gel electrophoresisProcedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel10
6769313408southern blottingA hybridization technique that enables researchers to determine the presence of certain nucleotide sequences in a sample of DNA.11
6769313409reverse transcriptase- PCRAn enzyme encoded by some certain viruses (retroviruses) that uses RNA as a template for DNA synthesis.12
6769313410Genetic profileAn individual's unique set of genetic markers, detected most often today by PCR or, previously, by electrophoresis and nucleic acid probes.13

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