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DNA, RNA and Protein Synthesis Flashcards

Covers DNA Replication and Protein Synthesis terms meant to prepare for a practical lab exam.

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445666775transformationprocess in which one strain of bacteria is changed by a gene or genes from another strain of bacteria
445666776bacteriophagea virus that infects bacteria
445666777nucleotidemonomer of nucleic acids made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base
445666778purinedouble-ring nitrogenous base, found in DNA and RNA; either adenine or guanine
445666779pyrimidinea nitrogenous base that has a single-ring structure; one of the two general categories of nitrogenous bases found in DNA and RNA; thymine, cytosine, or uracil
445666780Rosalind FranklinWoman who generated x-ray images of DNA, she povided Watson and Crick with key data about DNA
445666781Chargaff's Rule[A]=[T] and [G]=[C], they pair up across from one another forming two strands also called base pairing.
445666782Watson & Cricknobel prize winners for correctly describing the structure of DNA as a double helix
445666783base pairingprinciple that bonds in DNA can form only between adenine and thymine and between guanine and cytosine
445666784chromatingranular material visible within the nucleus; consists of DNA tightly coiled around proteins
445666785histoneprotein molecule around which DNA is tightly coiled in chromatin
445666786replicationcopying process by which a cell duplicates its DNA
445666787mRNAtype of RNA that carries instructions from DNA in the nucleus to the ribosome
445666788rRNAtype of RNA that makes up part of the ribosome
445666789tRNAtype of RNA that carries amino acids to the ribosome
445666790transcriptionprocess in which part of the nucleotide sequence of DNA is copied into a complementary sequence in RNA
445666791RNA Polymerasean enzyme that hooks together the RNA nucleotides as they base pair along the DNA template
445666792DNA Polymeraseenzyme involved in DNA replication that joins individual nucleotides to produce a DNA molecule
445666793codonthree-nucleotide sequence on messenger RNA that codes for a single amino acid
445666794anticodongroup of three bases on a tRNA molecule that are complementary to an mRNA codon
445666795translationthe process whereby genetic information coded in messenger RNA directs the formation of a specific amino acid
445666796mutationchange in a DNA sequence that affects genetic information
445666797point mutationa mutation in which only one nucleotide or nitrogenous base in a gene is changed
445666798frameshift mutationmutation that shifts the "reading" frame of the genetic message by inserting or deleting a nucleotide
445666799DNA Helicasean enzyme that unwinds the DNA double helix during DNA replication
445666800Helicasean enzyme that untwists the double helix at the replication forks, separating the two parental strands and making them available as template strands
445666801Single-Strand Binding Proteinafter helicase separates the 2 parental strands, molecules of this then bind to the unpaired DNA strands, stabilizing them until they serve as templates for the synthesis of new complementary strands
445666802DNA Polymeraseelongation of new DNA at a replication fork is catalyzed by an enzyme called ______ _________
445666803DNA Ligasean enzyme that eventually joins the sugar-phosphate backbones of the Okazaki fragments
445666804PrimaseThis enzyme can start an RNA chain from scratch. It removies single-strand binding proteins from the DNA template and joins RNA nucleotides together one at a time, making a primer complementary to the template strand
445666805TopoisomeraseA protein that helps relieve strain in the double helix ahead of the replication fork by breaking, twisting, and rejoining the strand.
445666806AdenineNitrogen base that pairs with thymine
445666807ThymineA Nitrogen base found in DNA (but not in RNA) and derived from pyrimidine
445666808GuanineA Purine base found in DNA and RNA
445666809CytosineThe Purine base that pairs with Guanine with DNA
445666810BacteriophageViruses that infect bacteria
445666811CloningMaking a genetically identical copy of DNA or of an organism
445666812Deoxyribonucleic AcidA double-stranded, helical nucleic acid molecule capable of replicating and determining the inherited structure of a cell's proteins. DNA
445666813DNA LigaseA linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain.
445666814DNA PolymeraseAn enzyme that catalyzes the elongation of new DNA at a replication fork by the addition of nucleotides to the existing chain.
445666815DNA RepairThe removal and replacement of damaged DNA by the correct sequence
445666816DNA Replicationthe process of making a copy of DNA
445666817NucleotideThe building block of a nucleic acid, consisting of a five-carbon sugar covalently bonded to a nitrogenous base and a phosphate group.
445666818X-Ray Diffraction ImageFilm image of x-rays scattered by a crystalline sample the resulting pattern of streaks and dots can be used to calculate the spacing between the atoms in the crystal lattice.
445666819Base SequenceThe order of nitrogenous bases on a chain of DNA.
445666820Alkylating AgentAgent that prevents DNA replication
445666821AnticodonGroup of three bases on a tRNA molecule that are complementary to an mRNA codon
445666822Base-Pair SubstitutionA type of point mutation; the replacement of one nucleotide and its partner in the complementary DNA strand by another pair of nucleotides.
445666823CodonA three-nucleotide sequence that encodes an amino acid or signifies a start signal or a stop signal
445666824Deletion and Insertion of BaseCreates frame shift mutation
445666825ExonPiece of DNA that codes for protiens and is exported from the nucleus.
445666826Frameshift MutationsExtra base inserted into gene region. Insertion shifts the reading of the frame by one base, which DNA polymerases read nucleotide sequences in blocks of 3
445666827Gene MutationA change in the sequence of the bases in a gene, which changes the structure of the polypeptide that the gene codes for.
445666828Genetic CodeThe ordering of nucleotides in DNA molecules that carries the genetic information in living cells
445666829IntronPiece of DNA that doesn't code for protiens, and it stays in the nucleus.
445666830Ionizing RadiationEnough energy to knock electrons from atoms forming ions, (gamma-Xrays-UV)
445666831mRNAmessenger RNA; type of RNA that carries instructions from DNA in the nucleus to the ribosome
445666832Mutation RateProbability of spontaneous mutating
445666833Nonionizing RadiationExcites electrons and causes them to make new covalent bonds, which affects the 3-D structure of Proteins and Nucleic acids
445666834Promoterregion of DNA that indicates to an enzyme where to bind to make RNA
445666835Ribonucleic Acid(RNA) single-stranded nucleic acid that contains the sugar ribose
445666836RNA PolymeraseEnzyme similar to DNA polymerase that binds to DNA and separates the DNA strands during transcription
445666837rRNARibosomal RNA; type of RNA that makes up part of the ribosome
445666838TranscriptionProcess in which part of the nucleotide sequence of DNA is copied into a complementary sequence in RNA
445666839TranslationThe process whereby genetic information coded in messenger RNA directs the formation of a specific protein at a ribosome in the cytoplasm
445666840Transposons(jumping genes) short strands of DNA capable of moving from one location to another within a cell's genetic material
445666841tRNAshort-chain RNA molecules present in the cell that attach the correct amino acid to the protein chain that is being synthesized at the ribosome of the cell
445666842UracilNitrogen base that pairs with adenine in RNA. Replacement of Thymine
445666843The nucleic acids DNA and RNA serve as the blueprints forProteins
445666844DNA nucleotides include the five-carbon sugar known asDeoxyribose
445666845A molecule containing a five-carbon sugar, a phosphate group, and one of four nitrogenous bases A, T, G, or C is known as anucleotide
445666846RNA nucleotides include the five-carbon sugar known asRibose
445666847RNA has three of the DNA nitrogenous bases, but it also hasUracil
445666848In DNA ____ always pairs with _____, and ____always pairs with _____.A with T & G with C
445666849In RNA A always pairs with____.U
445666850__________ bonds hold the double helix togetherhydrogen bonds
445666851Adenine and Thymine have __________ hydrogen bonds connecting them.two
445666852Guanine and Cytosine have ________ hydrogen bonds connecting them.three
445666853. _________ are specific stretches of DNA that program amino acid sequences of proteins.DNA
445666854________ consists of a double strand of polynucleotidesmRNA
445666855. _________ consists of single strand of polynucleotides.RNA
445666856. ___________, a single strand of polynucleotides that is a complimentary copy of a single strand of DNA, leaves the nucleus through a pore in the nuclear membrane and attaches to a ___________ in the cytoplasm where it is used to build ____________, which link together to form proteins.mRNA Ribosome Amino Acids
445666857. The transfer of genetic information from DNA into an RNA molecule is known as _____________.Transcription
445666858The decoding of RNA language into protein language or the information carried by mRNA (messenger RNA) into proteins is known as ____________.Translation
445666859.________________ are based on the nitrogenous bases of the mRNA, which carry the genetic instructions for the amino acid sequence of a polypeptide chain.Triplet Codons
445666860. ___________ carries the anticodons at one end to the ribosome where it bonds to the triplet codons. At the other end of the tRNA is the amino acid based on the triplet codon it corresponds with.tRNA
445666861. __________ is the start triplet codon.AUG
445666862Its anticodon is __________.UAC
445666863. Stop codons are: ______,__________,_________UAA, UAG, UGA
445666864A, C, T, and G are ______________.nitrogenous bases
445666865. During replication, DNA unzips and each original parent strand pairs up with a copy. The parent strand and the copy form a ____________________.daughter strand
445666866When amino acids bond forming the primary structure of a protein (like a beaded necklace), the process is known as ________________________.Protein Synthesis
445666867The bonds that bind amino acids together to form proteins are known as _________________.Peptide bonds
445666868nucleotiderepeating subunits of nucleic acids (DNA & RNA)
445666869deoxyribosethe sugar found in DNA
445666870DNAmolecule that carries the genetic information for all life
445666871RNAnucleic acid that transmits the message from DNA to ribosomes for the making of proteins
445666872ribosethe sugar found in RNA
445666873transcriptionflow of genetic information from DNA to RNA
445666874translationprocess in which RNA directs the making of proteins
445666875codonthree-nucleotide sequence in mRNA that encodes an amino acid for the making of proteins
445666876DNA replicationthe process of making a copy of DNA
445666877double helix"twisted ladder" shape of DNA
445666878helicaseenzyme that separates strands of DNA before DNA replication
445666879DNA polymeraseenzyme that creates new strands of DNA during replication
445666880TranscriptionThe process of making mRNA from DNA in the nucleus.
445666881TranslationThe process of using tRNA and mRNA to put together amino acids at the ribosome.
445666882mRNAMolecule that brings the instructions to make a protein from the nucleus to the ribosome.
445666883tRNAMolecule that brings amino acids to the ribosome during translation.
445666884rRNARibosomes are made of this molecule.
445666885Types of RNAmessenger RNA, transfer RNA and ribosomal RNA
445666886Differences between RNA and DNARNA has ribose, RNA has uracil, RNA not a double helix
445666887anticodon3 bases on tRNA molecules that are complementary to codons
445666888codons3 bases on mRNA that code for an amino acid
445666889Amino acidsThe monomers of proteins
445666890TCCThe complementary DNA strand for the DNA sequence: AGG
445666891AUGThe complementary RNA sequence for the DNA seqeuence: TAC
445666892ThreeThe number of nucleotides that code for each amino acid
445666893RNA polymeraseEnzyme that makes mRNA from DNA during transcription.
445666894nucleusWhere in a eukaryotic cell transcription occurs.
445666895Ribosome or cytoplasmWhere in the cell translation occurs.
445666896AUCThe anticodon for UAG.
445666897PurinesTerm for nucleotide bases with 2 rings.
445666898PyrimidinesTerm for nucleotide bases with 1 ring.
445666899Adenine and guanineThe 2 DNA bases that are purines.
445666900Cytosine and thymineThe 2 DNA bases that are pyrimidines
445666901Adenine and ThymineComplementary bases in DNA that have 2 hydrogen bonds between them.
445666902Guanine and cytosineComplementary bases in DNA that have 3 hydrogen bonds between them.
445666903HydrogenWeak bonds between the 2 DNA strands.
445666904CovalentThe strong bonds between the nucleotides on one strand of DNA.
445666905sugar and phosphateMake up the backbone of DNA or RNA.
445666906DeoxyriboseThe sugar in DNA.
445666907RiboseThe sugar in RNA
445666908HelicaseEnzyme that unwinds and unzips the DNA helix during DNA replication
445666909EnzymesProteins that control chemical reactions in the cell.
445666910HormonesChemical messengers within organisms. Some proteins are these.
445666911HemoglobinProtein that carries oxygen in red blood cells.
445666912Sugar, phosphate and nitrogen base3 parts of a nucletide.
445666913DNA PolymeraseEnzyme that builds the complementary strand in DNA replication
445666914DeoxyriboseThe sugar in DNA nucleotides
445666915RiboseThe sugar in RNA nucleotides
445666916UracilRNA base complementary to Adenine
445666917Before cells divideWhen DNA replication occurs
445666918IntronPieces of mRNa that do not code for the protein. They stay in the nucleus
445666919ExonPieces of mRNA that do code for the protein. These are spliced together and go to the ribosome
445666920SubstitutionReplacing one nucleotide in DNA with another. Only affects one codon
445666921FrameshiftCaused by an insertion or deletion in DNA. affects all codons after the point of mutation
445666922During DNA replicationWhen mutations occur
445666923PhosphodiesterType of covalent bond between the 3' end of one nucleotide and the 5' end of another
445666924topoisomerase...relieves overwinding strain ahead of the replication forks by breaking, swiveling adn rejoining DNA strands
445666925sNRP...Small nucleotide ribonucleoprotein that recognize introns for splicing mRNA
445666926GriffithAccidently discovered transformation by combining a heat killed virulent strain of bacteria with a live non virulent strain
445666927AveryWorked off of Griffith--discovered that DNA was the transforming material by destroying DNA in once experiment and protein in another. Transformation could not happen without DNA
445666928Hershey and ChaseRadioactively labeled protein and DNA to see which enters cells when a virus infects a cell. Discovered it was DNA
445666929Meselson and StahlDetermined that DNA replication is semiconservative
445666930ChargaffDetermined that the amounts of A in a DNA molecule are equal to the amounts of T and the amount of G is equal to the amount of C
445666931FranklinUsed x ray crystallography to determine the helical shape of DNA
445666932Watson and CrickDetermined the structure of the DNA molecule
445666933missense mutationa point mutation where one amino acid is coded for instead of another
445666934nonsense mutationa point mutation that puts a stop codon in the middle of an amino acid sequence
445666935silent mutationa point mutation that results in the same amino acid being coded for
445666936TATA boxthe promoter sequence in eukaryotes
445666937RNA processingputting a 5' guanine cap and a poly A tail on the 3' end prior to the mRNA leaving the nucleus
445666938spliceosomesnRNPs and proteins that cut out introns from mRNA and join the adjacent exons together to make mature mRNA
4456669393' carboncarbon on the sugar in a nucleotide that has an OH group
4456669405' carboncarbon on the sugar in a nucleotide that has a phosphate group
445666941transcription factorsIn eukaryotes, proteins that mediate the binding of RNA polymerase to start transcription
445666942Okazaki fragmentDNA segments made in the lagging strand

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