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Early Life & Diversification of Prokaryotes Flashcards

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6086554957*Prokaryotes*3.5 billion year old single-cell organisms in Bacteria/Archea0
6086595336How did the first living cells appear?1. *Abiotic Synthesis* of amino acids and nitrogenous bases. 2. Joining of small molecules into *macromolecules* (protiens, nucleic acids, etc.) 3. *Self replicating* molecules allow *inheritance.*1
6086633005*Miller-Urey Experiment*Tested Oparin-Haldane Hypothesis, simulating what Earth's early environment was thought to be and tested the chemical origin of life under those conditions; resulted in creation of amino acids and other organic compounds.2
6086647504*Oparin-Haldane Hypothesis*Hypothesis based on Earth's early atmosphere that reducing environment (electron adding)3
6086666076What was the atmosphere composed in early Earth?♦ Little Oxygen ♦ Thick Water Vapor ♦ Nitrogen ♦ Carbon Dioxide ♦ Hydrogen ♦ Methane ♦ Ammonia4
6090333436Explain the *Abiotic Synthesis of Macromolecules*Synthesis of RNA monomers occurs by dripping solutions of amino acids or RNA nucleotides onto hot sand, clay, or rock produce polymers from monomers. These polymers acted as weak catalysts for reactions.5
6090699257*Protocells*Droplets with membranes that maintain an internal chemistry different from that of their surroundings; early protocells had self-replicating molecules and metabolism-like source6
6090786995VesiclesFluid-filled compartments enclosed by a membrane-like structure that could have conditions possible for protocells7
6090827224*RNA*First genetic material that performed many catalytic functions, playing major role in protein synthesis8
6090967775*DNA*More complex, stable, stronger genetic material with more accurate replication in more complex organisms today9
6090953865*Ribozymes*RNA catalysts; many can make complementary copies of short pieces of RNA.10
6091165157Relate *Natural Selection* & *RNA* ReplicationThrough natural selection, ribosomes capable of quick self replication and few errors (depending on environment) left the most descendant molecules.11
6090772173Example of How *Vesicles* Lead to *Protocells*Can form when lipids or other organic molecules are added to water, causing the mixture to form a bilayer (like plasma mambrane) and increases when montmorillonite clay added increases vesicle assembly (performed metabolic reactions using external reactants)12
6090704305*Protobionts*Aggregates of abiotically produced molecules surrounded by a membrane.13
6091181815*Fimbrae*Short, hairlike appendages that help stick bacteria to substrate or another bacteria EX: Gonorrhea Bacterium14
6091202276*Pilus*Longer appendages that pull 2 cells together before DNA transfer between them15
6091229727*Capsule*Sticky layer of polysaccharide or protein around bacteria cell wall that is dense and well defined16
6091364292*Slime Layer*Sticky layer of polysaccharide or protein around bacteria cell wall that is not well organized17
6091236979*Endospores*Multilayered, resistant cells that form as a result of water being removed from endospore, the cell lyses, and an endospore is released. They only die in very extreme environmental conditions.18
6091241976*Peptidoglycan*Cell walls of bacteria composed of this polymer made of modified sugars by polypeptides19
6091247057*Gram-posative*Have simpler walls with larger amounts of peptidoglycan20
6091254785*Gram-negative*Have less peptidoglycan and are structurally more complex ♦ Lipopolysaccharide outer membrane21
6091319014How are *gram-negative* bacteria medically harmful?They are more resistant because of their protective *outer membrane* against bodily defenses. They are toxic causing fever and shock.22
6090749999*Cyanobacteria*Found to be same age as *stromatolites* and are the first organisms to release oxygen to Earth's atmosphere during the water-splitting step of photosynthesis (3.4 year old fossil in South Africa)23
6090631196*Genetic Recombination*The combining of DNA from two sources24
6090647995*Genetic Recombination* in Eukaryotes*Meiosis* and *fertilization* occur, bringing together DNA from different individuals25
6090650331*Genetic Recombination* in Prokaryotes♦ Transformation ♦ Transduction ♦ Conjugation26
6090458624*Transformation*Genotype of prokaryote altered by intake of foreign DNA from surroundings. A nonpathogenic cell incorporates foreign DNA by homologous DNA exchange.27
6090530901*Transduction*Viruses that infect bacteria (*phages*) take prokaryotic genes from one host cell to another, caused by phage replication accidents; virus may not be able to replicate because of lack of genetic material.28
6090562571*Conjugation*DNA transfers between 2 prokaryotic cells temporarily joined EX: E. Coli attaches pilus to recipient, forming "temporary mating bridge" and transfers DNA from donor to recipient.29
6090626588*Horizontal Gene Transfer*Transfer of DNA between different species; explains the diversity of prokayotes EX: Transformation, transduction and conjugation30
6091444423*Taxis*Direct movement toward or away from a stimulus EX: Prokaryotes that exhibit *chemotaxis* change their movement pattern based on how harmful the chemical is that they are near31
6091458417*Flagella*Helps in cell motility (locomotion); arose independently in Eukaryotas and and Prokaryotes. Main parts: motor, hook, filament (e)32
6091509652*Nucleiod*Single chromosome unenclosed by membrane (d)33
6091559486*Plasmids*Smaller independent replicating DNA molecules in prokaryotes34
6091553189*Obligate Aerobes*Must use oxygen for cellular respiration35
6091599053*Anaerobic Respiration*Substances other than oxygen accept electrons in electron transport chain36
6091612524*Nitrogen Fixation*Cyanobacteria convert atmospheric N2 into NH3 later into organic compounds especially by microorganisms37
6091648181Impact of *Nitrogen Fixation*Can increase nitrogen available for plants, which can't use atmospheric N2.38
6091660288*Heterocysts*Specialized cells that carry out nitrogen fixation39
6091682158*Biofilms*Metabolic cooperation between different prokaryotes occurring in surface-coating colonies. They can cause health and industrial problems.40
6091712933*Binary Fission*Single prokaryotic cells divides into 2 then 4 then 6 then 8, etc.41
6091736759Why haven't prokaryotes overtaken the Earth completely if they replicate so fast?They end up exhausting their nutrient supply, poisoning themselves with metabolic waste, facing other competition, or are consumed by other organisms42
6091760409What three factors caused prokaryotes to have high genetic variation, leading to rapid evolution?♦ Rapid Reproduction ♦ Mutation ♦ Genetic Recombination43
6091821399PhotoautotrophOrganism that must take in light for energy who's carbon source is CO2 or related compounds EX: Plants44
6091824929ChemoautotrophOrganism that must take in inorganic chemicals (like NH3) for energy who's carbon source is CO2 or related compounds EX: Some Prokaryotes (Sulfolobus)45
6091828426PhotoheterotrophOrganism that must take in light for energy who's carbon source is organic compounds EX: Some Marine Prokaryotes46
6091832131ChemoheterotrophOrganism that must take in organic compounds for both energy and as a carbon source EX: Animals47
6092446502*F-Factor*Pili can form and donate DNA because the presence of a certain piece of DNA48
6092465655*F-Plasmid*F-Factor in plasmid form → F- means lack of F-factor (recipient cell) → F+ means present F-factor (donor cell)49
6092558864*R Plasmids*Bacteria with resistant genes, coding for enzymes that hinder/destroy antibiotic effectiveness that are selected to survive and pass genes EX: Tetracyline50
6092727773MonophyleticALL descendants came from one common ancestor EX: *Cyanobacteria*51
6092748114What method would you use to best identify the diversity of bacterial and archaeal species in the enviornment?Metogenomics52
6093296169*Extremophiles*First prokaryotes assigned to Archean domain, that can live in the most extreme environments53
6093390970*Extreme Halophiles*Organisms that live in high salinity levels like the Dead Sea or Great Salt lake that either can tolerate it or need it to survive EX: Halobacterium→ can thrive in extreme salt environment, but die at levels less than 9%54
6093473329*Extreme Thermophiles*Thrive under extreme heat conditions that, in a normal cell, would easily break the DNA double helix EX: Sulfolobus→ have structural/chemical adaptations to maintain stability of DNA55
6094049270*Parasitism*Involves one organism living at the expense of another organism.56
6094088464*Commensalism*Involves one organism benefiting and the other being inaffected57
6094073685*Mutualism*Beneficial ecologycal interaction of 2 species EX: Bacteroides thetaiotaomicron in human intestines58
6094465966*Endotoxins*Lipopolysaccharide components of the outer membrane of gram-negative bacteria, only released when bacteria dies EX: Salmonilla→ found in food prepared without sanitation59
6094465967*Exotoxins*Proteins secreted by certain bacteria and other organisms. EX: Cholera→ exotoxin release causing Cl- ion to be released into gut EX: Botulinum→ caused by improperly can food; it's still harmful even if bacteria causing release isn't present60
6094514896How does *horizontal gene transfer* increase harmfulness of *pathogenic bacteria*?They can transfer new, host-harming genes that make otherwise harmless bacteria, harmful.61
6094208030*Bioremediation*Use of organisms to remove pollutants from soil, air, or water EX: Fertilizers sprayed on oil spill to increase native bacteria growth that metabolize the oil62

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