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Lecture Notes Restriction Fragment Length Polymorphism (RFLP)? Restriction Enzymes Restriction enzymes are bacterial enzymes that recognize specific (usually short) double stranded DNA sequences (dsDNA) and cut at or near this sequence. The area or site where this enzyme will act is called the Restriction site. It is obvious thus that it will only act on its restricted site. These enzymes were named after the bacteria of origin, example: the enzyme EcoRI was named because it was extracted from E. coli.

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