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Biochemistry Flashcards

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14101331734primary structuresequence of amino acids0
14101331753secondary structureH-bonding interactions form alpha helix and beta sheets1
14101339561tertiary structurefurther folding of the secondary structure of proteins involving interactions between R-groups hydrophilic/phobic, disulphide bridges2
14101348388quaternary structureResults from two or more polypeptide subunits.3
14101356035steroidslipids characterized by a carbon skeleton consisting of four fused rings4
14101358461Triglyceridesan energy-rich compound made up of a single molecule of glycerol and three molecules of fatty acid.5
14101360589Terpenesrepeating units of isoprene6
14101369722Increasing fluidity of cell membraneincreasing the unsaturated FA's, increasing cholestrol, shorter FA chains7
14101387545Competitive inhibitors effect on V-SNo change in Vmax, increase Km8
14101394818Non competitive inhibitors effect on V-SLower Vmax, no change in Km9
14101401699Uncompetitive inhibitors effect on V-SLower Vmax, decrease Km10
14101417680Metabolic catalysis pathways of glucoseglycolysis, glycogenesis, pentose phosphate pathway11
14101429468Metabolic anabolism of glucosegluconeogenesis, glycogenolysis12
14101442050Use of Acetyl CoAKrebs, ETC, ketogenesis, FA anabolism13
14101449848Making Acetyl CoAPDC, ketogenesis, beta-oxidation14
14101460868Enzyme for glucose->G6Phexokinase (HK)15
14101465579Enzyme for F6P -> F1,6BPphosphofructokinase (PFK)16
14101472401Net energy from glycolysis2 ATP and 2 NADH17
14101477210Enzyme for PEP -> Pyruvatepyruvate kinase18
14101657566products of fermentationNAD+, and lactate or ethanol19
14101670307PDC reactantsPyruvate, CoA-SH, NAD+20
14101673815PDC productsAcetyl Co-A, NADH, CO221
14101676860PDC locationmitochondrial matrix22
14101680744Krebs cycle reactants2 Acetyl CoA23
14101689990The Krebs cycle produces2 GTP, 6NADH, and 2FADH + OAA24
14101696870electron transport chainseries of electron carrier proteins that shuttle high-energy electrons during ATP-generating reactions25
14101941584ATP yield for mitochondrial NADH2.5 ATP per NADH26
14101945403ATP yield for cytoplasmic NADH1.5 ATP per NADH27
14101950720ATP yield for FADH21.5 ATP per FADH228
14101955181Enzyme for making ATP in ETCATP synthase29
14101963009Net ATP of cellular respiration3030
14101969128Gluconeogenesis reaction2 pyruvate -> 1 glucose31
141019758782 pyruvate + 2 ATP + 2 CO2 (gluconeogenesis step 1)2 OAA + 2 ADP (requires pyruvate carboxylase)32
141019842732 OAA + 2 GTP (gluconeogenesis step 2)2 PEP + 2 CO2 + 2GDP (requires PEP carboxylkinase)33
14102001706F1,6BP -> F6P enzyme in gluconeogenesisF1,6BPase34
14102008866G6P to Glucose enzymeglucose-6-phosphatase35
14102014673Glucose regulation: ATPInhibits PFK (glycolysis), activates F1,6BPase (GNG)36
14102019658Glucose regulation: citrateInhibits PFK (glycolysis), activates F1,6BPase (GNG)37
14102032330Glucose regulation: AMPInhibits F1,6BPase (GNG), activates PFK (glycolysis)38
14102040050Glucose regulation: F2,6BPInhibits F1,6BPase (GNG), activates PFK (glycolysis)39
14102046154Insulin glucose regulationIncrease glycolysis40
14102050113Glucagon glucose regulationIncrease GNG41
14102052061GNG locationliver42
14102062145Glycogenesis G6P->GIP enzymephosphoglucomutase43
14102067827Glycogenesis GIP+ UTP->UDP-glu enzymeUDP-glu pyrophosphorylase44
14102078804Glycogenesis UDP-glu -> glycogen enzymeglycogen synthase45
14102087900Glycogenolysis glycogen -> glycogen(n-1) +GIP enzymeglycogen phosphorylase46
14102094800Glycogenolysis GIP -> G6P enzymephosphoglucomutase47
14102100560Glycogen regulation: insulinInhibits glycogen phosphorylase (lysis); activates glycogen synthase (genesis)48
14102107737Glycogen regulation: glucagonInhibits glycogen synthase (genesis); activates glycogen phosphorylase (lysis)49
14102114063Glycogen regulation: epinephrineInhibits glycogen synthase (genesis); activates glycogen phosphorylase (lysis)50
14102121611Pentose Phosphate Pathway PPP goals1) produce ribose R5P + NADPH 2) support nt and FA synthesis by providing reducing E51
14102146519Oxidative phase of PPPIrreversible; G6P+ 2NADP+ -> R5P + 2 NADPH + CO252
14102156557Oxidative phase of PPP enzymeglyceraldehyde 6P dehydrogenase53
14102175646Non-oxidative phase of PPPReversible; R5P -> metabolic intermediates: nucleotides or F6P, GAP54
14102193280Enzyme to break down triglycerideslipase55
141022151133FA -> Fatty acyl co-A requirementsAcyl CoA synthase +CoA-SH+ATP56
14102280845How does fatty acyl co-A enter mitochondrial matrix from cytoplasm?Carnitine Shuttle57
141022836874 steps of beta oxidation1. oxidation 2. hydration 3. oxidation 4. cleavage58
14102297814Beta oxidation reactantsFatty acyl CoA, FAD+, H2O, NAD+, CoA-SH59
14102310335Beta oxidation productsFatty acyl CoA, Acetyl coA (2C), FADH2, NADH60
14102322100How many rounds of beta oxidation for n carbons in molecule?carbons/2 -161
14102327493Where does fat anabolism take place?cytoplasm62
14102337478first step fat anabolism + enzymeacetyl coA + CO2; acetyl CoA carboxylase63
14102347607Fat anabolism regulation: insulinactivates acetyl CoA carboxylase64
14102350827Fat anabolism regulation: glucagoninhibits acetyl CoA carboxylase65
14102358684Fat anabolism steps (starting from malonyl coA)1) elongation 2) reduction 3) dehydration 4) reduction66
14102383674Fat anabolism reactants (starting from malonyl coA)FA chain + 2 NADPH67
14102392564Fat anabolism products (starting from malonyl coA)H2O + CO2 + 2 NADP+68
14102399468Ketogenesis purposeprovides E to crucial organs (FA can't cross blood-brain barrier)69
14102411159Fate of amino acids1) new proteins 2) deamination a- NH3 goes to urea cycle b- C skeleton goes to acetyl coA or glucose70

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