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DNA polymerase III holoenzyme

Ps2 soln

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DNA Replication
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Replication fork
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DNA polymerase III holoenzyme
Polymerase
Dam
Replisome

H Solutions for Practice Problems for Molecular Biology, Session 2: DNA Replication Question 1 ATP * * H a) Box the part that is added to a growing chain of nucleic acid. b) Star the atom(s) that can form a hydrogen bond with the complementary nitrogenous base. c) Circle the part of the molecule that decreases the stability of RNA as compared to DNA.

PS2

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DNA Replication
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Polymerase
DNA polymerase III holoenzyme
Circular bacterial chromosome

Practice Problems for Molecular Biology, Session 2: DNA Replication Question 1 ATP ? a) Box the part that is added to a growing chain of nucleic acid. b) Star the atom(s) that can form a hydrogen bond with the complementary nitrogenous base. c) Circle the part of the molecule that decreases the stability of RNA as compared to DNA. d) Draw an arrow to the part of this molecule that you would modify to prevent further elongation. Indicate what change you would make next to the arrow drawn. Question 2 Consider the following origin of replication that is found on a chromosome. The sequence of region 1 is shown. Region 1: 5??CTGACTGACA?3?

Chapter 16 Molecular Basis of Inheritance

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Okazaki fragments
DNA polymerase
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DNA

Chapter 16 The Molecular Basis of Inheritance Sugar?phosphate backbone 5? end Nitrogenous bases Thymine (T) Adenine (A) Cytosine (C) DNA nucleotide Phosphate 3? end Guanine (G) Sugar (deoxyribose) LE 16-8 Adenine (A) Thymine (T) Guanine (G) Cytosine (C) Sugar Sugar Sugar Sugar 5? end 3? end 5? end 3? end Space-filling model Partial chemical structure Hydrogen bond Key features of DNA structure 0.34 nm 3.4 nm 1 nm Structure of DNA The parent molecule has two complementary strands of DNA. Each base is paired by hydrogen bonding with its specific partner, A with T and G with C. The first step in replication is separation of the two DNA strands. The parent molecule has two complementary
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