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Competitive ELISA of Digoxin

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Sahithi Battula Lab Partner: Antoine Le Chem 3500L 01 November 2011 Competitive ELISA of Digoxin Data Analysis It appears as though the replicates are not very far off from each other. After the two outliers were eliminated from the data, the highest range was 0.242. Although this is a high range when compared to the magnitude of the presented values, the average range between the duplicates in one column is only about 0.091. The two outliers that were removed through the Q test were 0.210 from the first column with a concentration of 10000 ng/mL of digoxin, which had a Q value of 0.816, and 0.372 from the 5000 ng/mL digoxin, which had a Q value of 0.868. Conc. (ng/mL) Absorbance Values Range (before Q test) Range (after Q test) Qgap (min) Qgap (max) 10000 0.172 0.173

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