Terms : Hide Images
| 2901042978 | foreign DNA | Restriction enzymes prevent infection from: | 0 | |
| 2901043770 | prokaryotic | Restriction enzymes are only found in this type of cells: | 1 | |
| 2901045055 | bacteriophages | A common vector of foreign DNA to bacterial cells are: | 2 | |
| 2901046430 | lytic cycle | Bacteriophages infect, reproduce within, and then destroy bacterial cells by this process: | 3 | |
| 2901046924 | endonucleases | Restriction enzymes are also termed: | 4 | |
| 2901050129 | cutting | Restriction enzymes attack foreign DNA by recognizing and ________ it at certain sequences. | 5 | |
| 2901051015 | 4,6 | Restriction enzymes recognize specific sequences that are __ or __ base pairs long. | 6 | |
| 2901052390 | restriction sites | Sequences which a restriction enzyme reliably cuts are termed: | 7 | |
| 2901054648 | genus and species of origin | The first three letters of a restriction enzyme refer to: | 8 | |
| 2901056699 | water, buffer, dna, restriction enzyme | A typical restriction digest consists of these parts: | 9 | |
| 2901058519 | enzyme cofactors | The buffer used for a digest controls the pH and provides: | 10 | |
| 2901059889 | 37C | The optimal temperature for most restriction enzymes is: | 11 | |
| 2901060468 | sticky ends, blunt ends | There are two types of digest products: | 12 | |
| 2901084941 | sticky ends | In this graphic, which type of digest product is indicated by A? |  | 13 |
| 2901084942 | blunt ends | In this graphic, which type of digest product is indicated by B? | | 14 |
| 2901122485 | hybridize | Sticky end products are much easier to: | 15 | |
| 2901124019 | DNA ligase | This enzyme joins digest fragments to form recombinant molecules: | 16 | |
| 2901126991 | recombinant | DNA produced by hybridization, which is pieced together from different sources is: | 17 | |
| 2901129176 | flexible | While blunt ends are more difficult to join chemically, they are also more ____ in their use. | 18 | |
| 2901130460 | bacterial plasmid | Large amounts of a desired DNA sequence or protein can be produced by ligating it into a(n) __________ and allowing the host cell to replicate it. | 19 | |
| 2901180717 | restriction map | A(n) ______ can be produced by subjecting a plasmid to several restriction enzymes and then comparing the sizes of the fragments produced. | 20 | |
| 2901181273 | electrophoresis | This is a method of separating DNA fragments: | 21 | |
| 2901181706 | size, charge | Electrophoresis separates fragments by ____ and ____. | 22 | |
| 2901182475 | point mutations on restriction sites | Restriction digest can be used in the clinical lab to detect: | 23 | |
| 2901187654 | electrophoresis, transfer to membrane | Blot tests consist of two parts: | 24 | |
| 2901184447 | membrane | In a blot test, DNA is separated by electrophoresis and then wicked onto a(n): | 25 | |
| 2901186026 | probe | In molecular biology, the term _____ refers to a sequence of ss-DNA which is labeled with an enzyme, luminescent chemical, or radioisotope. | 26 | |
| 2901187119 | hybridize | In a blot test, labeled probes _____ with their complimentary strand, which allows the bands to be visualized. | 27 | |
| 2901189256 | southern blot | This test involves electrophoresis, blotting, and labeling to detect DNA: | 28 | |
| 2901191272 | northern blot | This test involves electrophoresis, blotting, and labeling to detect RNA: | 29 | |
| 2901192268 | western blot | This test involves electrophoresis, blotting, and labeling to detect protein: | 30 |
