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AP Biology Ch 17: Biotechnology Flashcards

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8692834749Recombinant DNADNA produced by combining DNA from different sources0
8692834750BiotechnologyA form of technology that uses living organisms, usually genes, to modify products, to make or modify plants and animals, or to develop other microorganisms for specific purposes.1
8692834751PlasmidsSmall rings of DNA found naturally in some bacterial cells in addition to the main bacterial chromosome. Can contain genes for antibiotic resistance, or other "contingency" functions.2
8692834752Gene CloningThe production of multiple copies of a gene.3
8692834753Restriction enzymesEnzyme that cuts DNA at a specific sequence of nucleotides4
8692834754Sticky endA single-stranded end of a double-stranded DNA restriction fragment.5
8692834755DNA LigaseA linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain.6
8692834756Ti PlasmidPlasmid that infects plants- can carry new genes into mature plants cells7
8692834757Gene gunGun that shoots gold particles with plasmids attached- way to get genes into plant cells where Ti plasmid doesn't work, like wheat and corn8
8692834758polymerase chain reaction (PCR)A method of producing thousands of copies of DNA segment using the enzyme DNA polymerase9
8692834759gel electrophoresisProcedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel. Larger fragments move slower, DNA moves towards positive charge because DNA is negative10
8692834762Genetic profileAn individual's unique set of genetic markers, detected most often today by PCR or, previously, by electrophoresis and nucleic acid probes.11

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