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AP Biology - Chapter 20 Flashcards

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9531286126Recombinant DNADNA produced by combining DNA from different sources0
9531286127BiotechnologyA form of technology that uses living organisms, usually genes, to modify products, to make or modify plants and animals, or to develop other microorganisms for specific purposes.1
9531286128PlasmidsSmall rings of DNA found naturally in some bacterial cells in addition to the main bacterial chromosome. Can contain genes for antibiotic resistance, or other "contingency" functions.2
9531286129Gene CloningThe production of multiple copies of a gene.3
9531286130Restriction enzymesEnzyme that cuts DNA at a specific sequence of nucleotides4
9531286131Sticky endA single-stranded end of a double-stranded DNA restriction fragment.5
9531286132DNA LigaseA linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain.6
9531286133Cloning vectorDNA molecules that can carry foreign DNA into a host cell and replicate there.7
9531286134Complimentary DNA (cDNA)is made from mRNA by reverse transcriptase8
9531286135polymerase chain reaction (PCR)A method of producing thousands of copies of DNA segment using the enzyme DNA polymerase9
9531286136gel electrophoresisProcedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel10
9531286137southern blottingA hybridization technique that enables researchers to determine the presence of certain nucleotide sequences in a sample of DNA.11
9531286138reverse transcriptase- PCRAn enzyme encoded by some certain viruses (retroviruses) that uses RNA as a template for DNA synthesis.12
9531286139Genetic profileAn individual's unique set of genetic markers, detected most often today by PCR or, previously, by electrophoresis and nucleic acid probes.13

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