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Ap Biology Chapter 20 Flashcards

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6151708666Recombinant DNANucleotide sequences from two ifferent sources are combined in vitro into the same DNA molecule0
6151716308Genetic engineeringThe direct manipulation of genes for practical purposes1
6151718854BiotechnologyThe manipulation of organisms of their genetic components to make useful products2
6151724403PlasmidsSmall circular DNA molecules that replicate separately from the bacterial chromosome3
6151731628Gene cloningInvolves using bacteria to make multiple copies of a gene4
6151742408Restriction enzymesEnzymes that cut DNA molecules at specific DNA sequences5
6151748748Restriction fragmentsThe fragments of DNA that are produced from restriction enzymes6
6151754276DNA ligaseAn enzyme that seals the bonds between restriction fragments7
6151758805Cloning vectorThe original plasmid used in gene cloning8
6151767803Genomic libraryThe collection of recombinant vector cones produced by cloning DNA fragments from an entire genome9
6151777634Bacterial artificial chromosome (BAC)A large plasmid that has been trimmed down and can carry a large DNA insert10
6151790243Complimentary DNA (cDNA)A library that is made by cloning DNA made in vitro by reverse transcription of all the mRNA produced by a particular cell and only represents a part of the genome11
6151813686Nucleic acid hybridizationThe process that allows researchers to identify a gene of interest12
6151843586Yeast artificial chromosomes (YAC)A host cell that avoids gene expression13
6151852698ElectroporationA method to introduce recombinant DNA into eukaryotic cells by applying a brief electrical pulse to create temporary holes in plasma membranes14
6151872413Polymerase chain reaction (PCR)A way to produce many copies of a specific target segment of DNA15
6151882714Gel electrophoresisA method of rapidly analyzing and comparing genomes16
6151887835Southern blottingA technique that combines gel electrophoresis of DNA fragments with nucleic acid hybridization17
6151900391Northern blottingA technique that combines gel electrophoresis of mRNA followed by hybridization with a probe on a membrane18
6151916664DNA microarray assaysCompare patterns of gene expression in different tissues, at different times, or under different conditions19
6151924589In vitro mutagenesisMutations are introduced into a clonedd gene, altering or destroying its function20
6151933353TotipotentA cell that can generate a complete new organism21
6151939605RNA interference (RNAi)A way that gene expression is silenced by synthetic double stranded RNA molecules matching the sequence of a particular gene22
6151955485Stem cellsA relatively unspecialized cell that can reproduce itself indefinitely and differentiate into specialized cells of one or more types23
6151973334Single nucleotide polymorphins (SNPs)Single base-pair sites that vary in a population24
6151989108Gene therapyThe alteration of an afflicted individual's genes25
6151994973Transgenic animalsWhen genes from one species is introduced into the genome of another animal26
6152003833Genetic profileAn individual's unique DNA sequence27
6152009617Short tandem repeats (STRs)Variations in the number of repeats of specific DNA sequences28
6152017571Ti plasmidThe most commonly used vector for introducing new genes into plant cells29
6152024846Genetically modified organisms (GMOs)An organism whose genetic material has been altered through some genetic engineering technology or technique30

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