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| 8488847276 | Gs | stimulates adenylate cyclase, which increases levels of cAMP in the cell | 0 | |
| 8488851303 | Gi | inhibits adenylate cyclase, which decreases levels of cAMP in the cell | 1 | |
| 8488856531 | Gq | activates phospholipase C, which cleaves a phospholipid from the membrane to form PIP2. PIP2 is then cleaved into DAG and IP3. IP3 can open calcium channels in the ER, increasing Calcium levels in the cell. | 2 | |
| 8488883858 | G protein-coupled receptors | large family of integral membrane proteins involved in signal transduction. | 3 | |
| 8488889974 | Enzyme-linked receptors | membrane receptors with a catalytic activity. 3 primary protein domains: membrane-spanning, ligand-binding and catalytic | 4 | |
| 8488937159 | Electrophoresis | moves proteins according to charge and sz | 5 | |
| 8488950515 | Native PAGE | mass-to-charge or mass-to-sz ratios. Protein can be recovered. | 6 | |
| 8488959919 | SDS- PAGE | Separates proteins on the basis of molecular mass alone. SDS covers entire protein in negative charge | 7 | |
| 8488972867 | What is the average molar mass of one amino acid? | 100 daltons or 100g/mol | 8 | |
| 8488980470 | Isoelectric focusing | separating on the basis of pI. Say you have a protein with the pI of 9(basic) | 9 | |
| 8489012204 | When is chromatography preferred over electrophoresis? | When large amounts of protein are being separated | 10 | |
| 8489078129 | X-ray crystallography | the most reliable and common method of identifying protein structure(75%). measures electron density on an extremely high resolution scale and can also be used for nucleic acids. | 11 | |
| 8489088885 | NMR spectroscopy | to find 25% of protein structures- | 12 | |
| 8489097094 | Edman degradation | small proteins best analyzed. 50-70 aa. selectively and sequentially removes the N-terminal aa which can then be analyzed via mass spec | 13 |
