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Biochemistry Flashcards

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3823670383What is the most common intracellular buffer?Protein0
3823670384What is the most common extracellular buffer?Bicarbonate1
3823670385What is a Zwitterion?A molecule with one negative and one positive end2
3823670386What is the Isoelectric Point?The pH at which there is no net charge3
3823670387What is the rate limiting enzyme in Glycolysis?PFK-14
3823670388What is the rate limiting enzyme in Gluconeogenesis?Pyruvate carboxylase5
3823670389What is the rate limiting enzyme in the HMP shunt?G-6PD6
3823670390What is the rate limiting enzyme in Glycogenesis?Glycogen synthase7
3823670391What is the rate limiting enzyme in Glycogenolysis?Glycogen phosphorylase8
3823670392What is the rate limiting enzyme in FA synthesis?AcCoA carboxylase9
3823670393What is the rate limiting enzyme in β oxidation?CAT-110
3823670394What is the rate limiting enzyme in Cholesterol synthesis?HMG CoA reductase11
3823670395What is the rate limiting enzyme in Ketogenosis?HMG CoA S'nd1ase12
3823670396What is the rate limiting enzyme in Purine synthesis?PRPP synd1ase13
3823670397What is the rate limiting enzyme in Pyrimidine synthesis?Asp transcarbamoylase14
3823670398What is the rate limiting enzyme in TCA cycleIsocitrate dehydrogenase?15
3823670399What is the rate limiting enzyme in Urea cycle?CPS-I16
3823670400What is the rate limiting enzyme in Heme synthesis?delta-ALA synthase17
3823670401What are the catabolic pathways that create energy?''ABC" AcetylCoA production β-oxidation Citric acid cycle18
3823670402What are the anabolic pathways that store energy? MUST EDIT"EFGH" ER fatty acid synthesis Glycolysis HMP shunt19
3823670403What are the anabolic + catabolic pathways?"HUG" Heme synthesis Urea cycle Gluconeogenesis20
3823670404What does an Isomerase do?Creates an isomer21
3823670405What does an Epimerase do?Creates an epimer, which differs around 1 chiral carbon22
3823670406What does a Mutase do?Moves sidechain from one carbon to another (intrachain)23
3823670407What does a Transferase do?Moves sidechain from one substrate to another (interchain)24
3823670408What does a Kinase do?Phosphorylates using ATP25
3823670409What does a Phosphorylase do?Phosphorylates using Pi26
3823670410What does a Carboxylase do?Forms C-C bonds (w/ ATP and biotin)27
3823670411What does a Synthase do?Consumes 2 substrates28
3823670412What does a Synthetase do?Consumes 2 substrates, uses ATP29
3823670413What does a Phosphatase do?Breaks phosphate bond30
3823670414What does a Hydrolase do?Breaks a bond with water31
3823670415What does a Lyase do?Cuts C-C bonds w/ ATP32
3823670416What does a Dehydrogenase do?Removes H with a cofactor33
3823670417What does a Thio do?Breaks S bonds34
3823670418What is Diffusion?From high to low concentration35
3823670419What is Active Transport?Goes against concentration gradient36
3823670420What is Zero-order kinetics?Metabolism independent of concentration37
3823670421What is 1st-order kinetics?Constant drug percentage metabolism over time, depends on drug concentration38
3823670422What is Efficacy?Max effect regardless of dose (lower w/ non-competitive antagonist)39
3823670423What effects Efficacy?Vmax40
3823670424What is Potency?amount of drug needed to produce effect (lower w/ comp antagonist)41
3823670425What affects Potency?Km42
3823670426What is Kd?Concentration of drug that binds 50% of receptors43
3823670427What is EC5o?Concentration of drug that produces 50% of maximal response44
3823670428What is Competitive Inhibition?Fights for active site, no change in Vmax, potency decreases45
3823670429What is Non-competitive Inhibition?Binds a regulatory site, no change in Km, efficacy decreases, decreased Vmax46
3823670430What is an Endothermic Reactionconsumes heat47
3823670431What is an Exothermic ReactionGives off heat48
3823670432What is the Peak level4 hrs after dose (too high=> decrease dose)49
3823670433What is the Trough level2 hrs before next dose (too high => give less often)50
3823670434What is t1/2Half-life, the time it takes for the body to use half of the drug ingested51
3823670435What is von GierkeG-6Pase deficiency= > hypoglycemia, hepatosplenomegaly52
3823670436What is Pompe'sCardiac alpha-1,4-glucosidase deficiency = > DIE early53
3823670437What is Cori'sDEbranching enzyme deficiency = > short branches of glycogen54
3823670438What is Anderson'sBranching enzyme deficiency = > long chains of glycogen55
3823670439What is McArdle'sMuscle phosphorylase deficiency = > muscle cramps w/ exercise56
3823670440What is Essential FructosuriaFructokinase deficiency => excrete fructose (still have hexokinase)57
3823670441What is Fructosemia"Fructose intolerance" (Aldolase B deficiency ) →liver damage58
3823670442What does a Galactokinase deficiency causeCataracts59
3823670443What does Galactosemia causeCataracts, mental retarda tion, liver damage60
3823670444What does the Citrate shuttle doFA transport out of the mitochondria61
3823670445What does the Carnitine shuttle doFA transport into the mitochondria62
3823670446What lysosomal diseases have a cherry-red maculaTay-Sachs, Niemann- Pick63
3823670447What lysosomal diseases have a Gargoyle faceGaucher's, Hurler's64
3823670448What is Tay-SachsHexosaminidase A deficiency=> blindness, incoordination, dementia65
3823670449What is Sandhoff'sHexosaminidase A/B deficiency66
3823670450What is Gaucher'sGlucocerebrosidase d eficiency => wrinkled tissue MP, bone pain67
3823670451What is Niemann-PickSphingomyelinase deficiency => zebra bodies68
3823670452What is Fabry'salpha-galactosidase deficiency=> corneal clouding, attacks baby's kidneys , X-linked69
3823670453What is Krabbe'sbeta-galactosidase deficiency=> globoid bodies70
3823670454What is Metachromatic LeukodystrophyArylsulfatase deficiency => childhood MS71
3823670455What is Hunter'sIduronidase deficiency, milder form72
3823670456What is Hurler'sIduronidase deficiency, worse form73
3823670457What is Lesch-Nyhan(HGPRT deficiency) =>gout, neuropathy, self-mutilation74
3823670458What do white diaper crystals suggestExcess orotic acid (pyrimidine synthesis pathway)75
3823670459What does biotin donate methyl groups forCarboxylation76
3823670460What does THF donate methyl groups forNucleotides77
3823670461What does SAM donate methyl groups forAll other reactions78
3823670462What is the difference b/w Heterochromatin and EuchromatinHeterochromatin = tightly coiled (30nm fibers) Euchromatin = loose ( 10nm fibers )79
3823670463What are the PurinesA,G80
3823670464What are the PyrimidinesC,U,T81
3823670465What is a silent mutationChanges leave the same amino acid82
3823670466What is a point mutationChanges 1 base83
3823670467What is a transitionChanges 1 purine to another purine84
3823670468What is a transversionChanges 1 purine to a pyrimidine85
3823670469What is a frameshift mutationInsert or delete 1 -2 bases86
3823670470What is a missense mutationMistaken amino acid substitution87
3823670471What is a nonsense mutationEarly stop codon88
3823670472What does a Southern blot detectDNA "SNOW DROP"89
3823670473What does a Northern blot detectRNA "SNOW DROP"90
3823670474What does a Western blot detectProtein "SNOW DROP"91
3823670475What are the essential amino acids"I saw he phoned at 3:09, and met licentious argentines - Lucy, Tracy and Val" Ile His Phe Thr Met Lys Arg Leu Trp Val92
3823670476What are the essential fatty acidsLinolenic, Linoleic93
3823670477What are the acidic amino acidsAsp, Glu94
3823670478What are the basic amino acidsLys, Arg95
3823670479What are the sulfur-containing amino acidsCys, Met96
3823670480What are the 0-bond amino acidsSer, Thr, Tyr97
3823670481What are the N-bond amino acidsAsp, Gln - Acidic98
3823670482What ate the branched amino acidsLeu, Ile, Val "I saw Lucy and Val"99
3823670483What are the aromatic amino acidsPhe, Tyr, Trp100
3823670484What is the smallest amino acidGly101
3823670485What are the ketogenic amino acidsLys, Leu102
3823670486What are the glucogenic + ketogenic amino acidsPITT Phe, Iso, Thr, Trp103
3823670487What are the glucogenic amino acidsAll the rest104
3823670488What amino acids does Trypsin cutLys, Arg - basic105
3823670489What amino acids does beta-ME cutCys, Met106
3823670490What amino acids does Acid Hydrolysis denatureAsp, Gln107
3823670491What·amino acids does Chymotrypsin cutPhe, Tyr, Trp - aromatic108
3823670492What amino acid turns yellow on Nurhydrin reactionPro109
3823670493What does Carboxyptidase cutLeft of any amino acid on th e carboxyl- terminal110
3823670494What does Aminopeptidase cutRight t of N terminus111
3823670495What does CNBr cutRight o f Met112
3823670496What does Mercaptoethanol cutRight of Cys, Met - sulfur113
3823670497What does Elastase cutRight of Gly, Ala, Ser114
3823670498What does Trypsin cutArg, Lys - basic115
3823670499What does Chymotrypsin cutPhe, Tyr , Trp - aromatic116
3823670500What does alpha1-AT doInhibits trypsin from getting loose117
3823670501What is PKUNo Phe --> Tyr (via Phe Hydroxylase) : Nutrasweet sensitivity, mental retardation , pale, blond hair, blue eyes, musty odor118
3823670502What is AlbinismNo Tyr--> Melanin (via Tyrosinase)119
3823670503What is Maple Syrup Urine diseaseDefective metabolism of branced aa (Leu, Iso, Val)=> aa leak out120
3823670504What is HomocystinuriaNo Homocystine --> Cys : "COLA" stones Cystine, Ornithine, Lysine, Arginine121
3823670505What is PellagraNiacin deficiency: • Dermatitis, Diarrhea , Dementia , Death122
3823670506What is Hartnup'sNo Tr p => Niacin + Serotonin • Presents like Pellagra • Can mimic corn-rich diet123
3823670507What causes anterior leg bowingNeonatal syphilis124
3823670508What causes lateral leg bowingRickets125
3823670509What are the names of the B vitamins"The Rich Never Lie about Panning Pyrite Filled Creeks" Vit B1 = Thiamine Vit B2 = Riboflavin Vit B3 = Niacin Vit B4, = Lipoic acid Vit B5 = Pantothenic acid Vit B6 = Pyridoxine Vit B9 = Folate Vit B12 = Cobalamin126
3823670510What does VitA doNight vision , CSF production , PTH127
3823670511Wha t does Vit B 1 doDehydrogenases, transketolasc (PPP) cofactors128
3823670512What does Vit B 2 doFAD cofactor129
3823670513What does Vit B3 doNAD cofactor130
3823670514What does Vit B 4 doGlycolysis, no known diseases131
3823670515What does Vit B 5 doPart of AcetylCoA, no known diseases132
3823670516What does Vit B 6 doTransaminase cofactor, myelin integrity133
3823670517What does Vit B 9 doNuclear division134
3823670518What does Vit B 12 doCofactor for HMT MMM135
3823670519What does Vit C doCollagen synthesis136
3823670520What does Vit D doMineralization of bones, teeth137
3823670521What does Vit K doClotting138
3823670522What does Biotin doCarboxylation139
3823670523What does Ca2+ doNeuronal function, atrial depobrization, skeletal muscle depolarization140
3823670524What does Cu2+ doCollagen synthesis141
3823670525What does Fe2+ doHb function, electron transport142
3823670526What is Bronze pigmentationFe deposit in skin143
3823670527What is Bronze cirrhosisFe deposit in liver144
3823670528What is Bronze diabetesFe deposit in pancreas145
3823670529What is HemosiderosisFe overload in bone marrow146
3823670530What is HemochromatosisFe deposit in organs147
3823670531What does Mg2+ doPTH and kinase cofactor148
3823670532What does Zn2+ doTaste buds, hair, sperm function149
3823670533What does Cr do (chromium)Insulin function150
3823670534What does Mo do (molybdenum)Purine breakdown (xanthine oxidase)151
3823670535What does Mn do (manganese)Glycolysis152
3823670536What does Se do (selenium)Heart function :::::> dilated cardiomyopathy153
3823670537What does Sn do (tin)Hair154
3823670538What is KwashiorkorMalabsorption, big belly (ascites), protein deficiency155
3823670539What is MarasmusStarvation, skinny, calorie deficiency156
3823670540Where does the Pre label send stuff toER157
3823670541Where does the Pro label send stuff toGolgi158
3823670542Where does the Mannose-6-P send stuff toLysosome159
3823670543Where does the N-terminal sequence send stuff toMitochondria160
3823670544What are the 4 types of collagen"SCAB" Type 1: Skin, bone Type II: Connective tissue, aqueous humor Type III: Arteries Type IV: Basement membrane161
3823670545How does Scleroderma presentTight skin162
3823670546How does Ehlers Danlos presentHyperstretchable skin163
3823670547How does Marfan's presentHyperextensible joints, arachnodactyly, wing span longer than height, Aortic root dilatation, aortic aneurysm, mitral valve prolapse, Dislocated lens from bottom of eye -> look up164
3823670548How does Homocystinuria presentDislocated lens from top -> look down165
3823670549How does Kinky hair disease presentHair looks like copper wire (Cu deficiency)166
3823670550How does Scurvy presentBleeding gums, bleeding hair follicles167
3823670551How does Takayasu arteritis presentAsian female with very weak pulse168
3823670552How does Osteogenesis lmperfecta presentShattered bones, blue sclera169

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