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Biochemistry Flashcards

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7224014728Kcat0
7224015741EfficiencyEfficiency = Kcat/Km1
7224017608Competitive inhibitorBinds free enzyme & competes with substrate 1. Higher apparent Km 2. Same Kcat 3. Same Vmax2
7224021003Uncompetitive inhibitorBinds active site of ES complex 1. Lower apparent Km 2. Lower Vmax3
7224029484Mixed inhibitorBinds enzyme & regulatory site of ES complex 1. Apparent change in Km 2. Lower Vmax4
7224030254Non-competitive inhibitorBinds enzyme & regulatory site of ES complex equally 1. Lower apparent Km 2. Lower Vmax5
7340043159Suicide inhibitor-Only inhibitor that can act as an irreversible enzyme inhibitor -Occurs when an enzyme forms an irreversible complex with the inhibitor (structurally a substrate analogue) via a covalent bond -Inhibitor may be chemically modified by the enzyme to produce a reactive group that is specifically responsible for the formation of the irreversible inhibitor-enzyme complex6
7224033870Lineweaver-Burk plotSlope = Km/Vmax7
7224038416Michaelis Menten curve*Uncompetitive moves left & down8
7246096088Hill coefficientHC = 1: no cooperativity HC > 1: cooperativity9
7250488988Glucagon-Released by pancreatic alpha cells -Increase blood glucose levels10
7250490491Insulin-Released by pancreatic beta cells -Decrease blood glucose levels11
7250473984Glycogenesis-Glucose molecules are added to chains of glycogen for storage via glycogen synthase -Activated by insulin12
7250504610Glycogenolysis-Breakdown of glycogen to glucose via glycogen phosphorylase -Activated by glucagon13
7250535679GluconeogenesisGeneration of glucose from non-carbohydrate sources: 1. Proteins: glucogenic amino acids 2. Lipids: triglycerides, glycerol 3. Other steps of metabolism: lactate, pyruvate14
7250575536Pentose phosphate pathway-Occurs in cytosol -Produces NADPH & pentose sugars for nucleotide biosynthesis Phase 1: Oxidative phase -> creation of NADPH (used as a reductive agent) Phase 2: Non-oxidative phase -> pentose synthesis15
7463781859Beta oxidation of fatty acids-Process by which fatty acid molecules are broken to generate acetyl-CoA (enters the citric acid cycle), NADH, & FADH2 -Occurs in cytosol in prokaryotes -Occurs in mitochondria in eukaryotes16
8020399748Glycolysis17
8513064141Electron carriers in ETC1. Complex I: pumps 4 protons into IMS; shuttles 2 electrons to Q (becomes QH2) 2. Complex II: shuttles electrons to Q 3. Ubiquinone (Q): accepts 2 electrons & is reduced to ubiquinol (QH2) 4. Complex III (cyt bc1 complex): pumps 4 protons into IMS; Q cycle shifts electrons from QH2 to cyt c 5. Cyt C: can only accept/transfer 1 electron 6. Complex IV: pumps 2 protons into IMS; 2 cyt c's reduce 1/2 O2 to 1 H2O18

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