Terms : Hide Images
| 11127842087 | herbert boyer was bron in | 1936 | 0 | |
| 11127842088 | the European Federation of Biotechnology | the integration of natural science and organisms cells parts thereof and molecular analogues for products and services | 1 | |
| 11127842089 | genetic engineering | techniques to alter the chemistry of genetic material dna and rna to introduce these into gost and thus change the phenotype | 2 | |
| 11127842090 | Bioprocess Technology | maintenance of sterile ambience in chemical engineering process to enable growth of only desired microbe | 3 | |
| 11127842091 | origin of replication | initiation of replication | 4 | |
| 11127842092 | Cloning | making multiple identical copies of any template dna | 5 | |
| 11127842093 | Plasmid | autonomously replicating circular extra Chromosomal dna | 6 | |
| 11127842094 | 1st plasmid | salmonella typhimurium | 7 | |
| 11127842095 | Stanley Cohen and herbert boyer in | 1972 | 8 | |
| 11127842096 | normal ecoli is | not antibiotic resistant | 9 | |
| 11127842097 | restriction enzyme | smith and nathan | 10 | |
| 11127842098 | bacteria protect themselves from viruses | by fragmenting viral dna with endonuclease | 11 | |
| 11127842099 | Molecular scissors | restriction enzymes | 12 | |
| 11127842100 | endonuclease is | nuclease | 13 | |
| 11127842101 | 1963 | two enzymes were discovered in | 14 | |
| 11127842102 | 1 endonuclease | hind || | 15 | |
| 11127842103 | methyl group | enzyme endonuclease cannot cut it's own DNA bcoz | 16 | |
| 11127842104 | ecoli strian | R | 17 | |
| 11127842105 | removed dna from ends | exonuclease | 18 | |
| 11127842106 | removes dna at specific points | endonuclease | 19 | |
| 11127842107 | palindromic nucleotide sequences | each restriction endonuclease recognize a specific | 20 | |
| 11127842108 | cohesive ends | sticky ends | 21 | |
| 11127842109 | gel electrophoresis | dna fragments are separated | 22 | |
| 11127842110 | agarose gel | sea weed | 23 | |
| 11127842111 | using ethidium bromide | dna is seen | 24 | |
| 11127842112 | the ser fragments | move farther | 25 | |
| 11127842113 | the separated bands of dna are cut out from the agarose gel and extracted from the gel piece | elution | 26 | |
| 11127842114 | transformation | process through which price of dna is introduces in a host bacteria | 27 | |
| 11127842115 | terR | BamH I Sal I | 28 | |
| 11127842116 | Bam HI | GGATCC | 29 | |
| 11127842117 | Bam H1 | Bacillus amyloliquefaciens | 30 | |
| 11127842118 | ampR | Pvu I Pst T | 31 | |
| 11127842119 | insertional inactivation | inactivation of gene synthesis of that enzyme | 32 | |
| 11127842120 | when big dna | BAC YAC | 33 | |
| 11127842121 | ss bacteriophage | m13 vector | 34 | |
| 11127842122 | ds bacteriophage | lambda phage | 35 | |
| 11127842123 | PUC | plasmid university of California | 36 | |
| 11127842124 | chromatogenic substance | change into blue by beta galactosidase | 37 | |
| 11127842125 | chromatogenic no color | a galactosidase | 38 | |
| 11127842126 | Agrobacterium tumefaciens | vector for ti plasmid | 39 | |
| 11127842127 | pcr by | kary mullis | 40 | |
| 11162407171 | gel electrophoresis by | tiselius | 41 |
