Chapter 20: DNA Technology and Genomics Vocab
135427784 | recombinant DNA | DNA derived from two different sources | |
135427785 | genetic engineering | the direct manipulation of genes | |
135427786 | Biotechnology | the manipulation of organisms or their components to make useful products | |
135427787 | gene cloning | The process of preparing well defined, gene sized pieces of DNA in multiple identical copies | |
135427788 | clone | identical cell | |
135427789 | restriction enzymes | enzymes that cut DNA at a specific sequence of nucleotides | |
135427790 | sticky end | an end of DNA in which one strand of the double helix extends a few units beyond the other | |
135427791 | DNA ligase | Enzyme that catalyzes t he formation of covalent bonds in the sugar-phosphate backbone | |
135427792 | restriction site | the specific site at which a restriction enzyme will cleave DNA | |
135427793 | restriction fragments | DNA segments resulting from cutting of DNA by a restriction enzyme | |
135427794 | cloning vector | the original plasmid | |
137674553 | nucleic acid hybridization | a gene is detected by its ability to base pair with a complementary sequence. | |
137674554 | nucleic acid probe | short strand of RNA or DNA (single strand). hydrogen bonds with gene in nucleic acid hybridization. labeled with radioactive or fluorescent tag. | |
137674555 | denaturation | the separation of the two strands of DNA in a cell. | |
137674556 | genomic library | the complete set of plasmid clones, each carrying copies of a particular segment from the initial genome. | |
137674557 | complementary DNA | cDNA. double stranded DNA. | |
137674558 | cDNA library | collection of cDNA clones, only part of the genome. |