DNA methylation

Campbell's Biology, 9e (Reece et al.) Chapter 20 Biotechnology The new questions in Chapter 20 cover all of the chapter?s concepts and are primarily at the higher skill levels. In addition, the chapter presents several scenarios that are accompanied by a series of questions. Multiple-Choice Questions 1) Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to A) insert the fragments cut with restriction enzyme X directly into the plasmid without cutting the plasmid.