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Recombinant DNA

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Chapter 20 Biotechnology Overview: The DNA Toolbox Sequencing of the human genome was completed by 2007 DNA sequencing has depended on advances in technology, starting with making recombinant DNA In recombinant DNA, nucleotide sequences from two different sources, often two species, are combined in vitro into the same DNA molecule Methods for making recombinant DNA are central to genetic engineering, the direct manipulation of genes for practical purposes DNA technology has revolutionized biotechnology, the manipulation of organisms or their genetic components to make useful products An example of DNA technology is the microarray, a measurement of gene expression of thousands of different genes Fig. 20-1

AP BIO Ch. 20 Study Guide

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AP BIOLOGY CHAPTER 20 DNA TECHNOLOGY AND GENOMICS PAGE 375 Mapping and sequencing of human genome has been accomplished Via recombinant DNA ?. Genes from two different sources Genetic engineering?? manipulaton of genes? Biotechnology ?..manipulation to make useful products? Microbes to make wine and cheese, selective breeding of livestock DNA Cloning Making multiple identical copies of gene-sized DNA A gene inserted into a plasmid Bacteria reproduces?.recombinant plasmid is replicated Diagram pg 376 Potential uses ?.. Produce a protein product Ex. Gene for human growth hormone Produce many copies of gene So the gene nucleotide sequence can be determined Or give an organism a new metabolic ability?.pest Resistance?.

DNA Technology and Genomics

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Chapter 20 DNA Technology and Genomics Overview: Understanding and Manipulating Genomes One of the great achievements of modern science has been the sequencing of the human genome, which was largely completed by 2003. Progress began with the development of techniques for making recombinant DNA, in which genes from two different sources?and often different species?are combined in vitro into the same molecule. The methods for making recombinant DNA are central to genetic engineering, the direct manipulation of genes for practical purposes. Applications include the introduction of a desired gene into the DNA of a host that will produce the desired protein.
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