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Antibody

Intro to Microbiology, Part 3/3 Study Guide

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Biology
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Molecular biology
Genetics
Biotechnology
Gene delivery
Emerging technologies
genetic engineering
Transgene
Gene therapy
Virus
Antibody
Gene knockout
Retrovirus

Intro to Biotechnology Mark Muller April 4, 2015 Chelsea Claverie Exam 3 Study Guide Southern blotting - Southern blot uses an autoradiograph in combination with an electrophoresis gel to identify similarities between DNA. Similar DNA should travel the same distance in the electrophoresis, on the blot, and have the same binding sites from probes on the autoradiograph. Retrovirus Life Cycle in Cells: Retrovirus originates in nucleus of cell, binds to cell receptor of new cell to infect, reverse transcripts, then binds to the host DNA

Intro to Microbiology, Part 2/3 Study Guide

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Biology
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Molecular biology
Plasmid
Restriction enzyme
Transformation
Gene expression
Molecular cloning
Antibody
Virus
DNA
Polymerase chain reaction
Restriction map
Genetics

Intro to Biotechnology Mark Muller March 19, 2015 Chelsea Claverie Midterm Study Guide The Northern Blot detects the presence of certain RNA sequences, while the Southern blot can detect various DNA segments. Northern blot uses a gel electrophoresis, blot, RNA probes, and x-ray to determine the presence of a particular mRNA sequence that was immobilized and run in the electrophoresis. Southern blot uses an autoradiograph in combination with an electrophoresis gel to identify similarities between DNA. Similar DNA should travel the same distance in the electrophoresis, on the blot, and have the same binding sites from probes on the autoradiograph.

ApBo

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immune system
anatomy
Immunology
Biology
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Antigenic variation
Antigen
Adaptive immune system
Autoimmunity
Antibody
Passive immunity
B cell
health

Chapter 43 TheImmuneSystem 43-3,4 Active/Passive Immunization Active immunity : defenses that arise when a pathogen infects the body and prompts a primary or secondary immune response Passive immunity : antibodies guard against pathogens that have never infected them (lasts as long as antibodies last) ? Example: mother?s IgG & IgA antibodies to baby Artificial active immunity: immunization (vaccination) Antigens used to make vaccines Artificial passive immunity: inject antibodies from an animal already immune into another animal ? Short-term but immediate Polyclonal/Monoclonal Antibodies : Polyclonal or monoclonal Polyclonal : products of many different clones of plasma cells Monoclonal : made from a single clone of B cells grown in culture
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