A Tour of the Cell
Ch. 7 Tour of the Cell 2007-2008 Light Microscope - LM ? Uses visible light ? inexpensive ? live or dead objects ? Resolution: ? As magnification ?, resolution ?. ? ~1000X. ? Limitations: ? many cell structures too small Just magnified 64X Magnification & Resolution Light Microscope Variations ? Unstained ? Stained: ?Methylene blue ? iodine Light Microscope Variations ? Fluorescence: uses dyes to make parts of cells ?glow?. ? Phase-contrast: enhances contrasts in density. ? Confocal: uses lasers and special optics to focus only narrow slides of cells. Electron Microscopes ? Use beams of e- ? Invented in 1939 ? Advantages: ?Much higher mag. ? 50,000X ? Disadvantages: ? Vacuum.