AP Notes, Outlines, Study Guides, Vocabulary, Practice Exams and more!

1.3 - Restriction Enzymes Flashcards

Terms : Hide Images
2901042978foreign DNARestriction enzymes prevent infection from:0
2901043770prokaryoticRestriction enzymes are only found in this type of cells:1
2901045055bacteriophagesA common vector of foreign DNA to bacterial cells are:2
2901046430lytic cycleBacteriophages infect, reproduce within, and then destroy bacterial cells by this process:3
2901046924endonucleasesRestriction enzymes are also termed:4
2901050129cuttingRestriction enzymes attack foreign DNA by recognizing and ________ it at certain sequences.5
29010510154,6Restriction enzymes recognize specific sequences that are __ or __ base pairs long.6
2901052390restriction sitesSequences which a restriction enzyme reliably cuts are termed:7
2901054648genus and species of originThe first three letters of a restriction enzyme refer to:8
2901056699water, buffer, dna, restriction enzymeA typical restriction digest consists of these parts:9
2901058519enzyme cofactorsThe buffer used for a digest controls the pH and provides:10
290105988937CThe optimal temperature for most restriction enzymes is:11
2901060468sticky ends, blunt endsThere are two types of digest products:12
2901084941sticky endsIn this graphic, which type of digest product is indicated by A?13
2901084942blunt endsIn this graphic, which type of digest product is indicated by B?14
2901122485hybridizeSticky end products are much easier to:15
2901124019DNA ligaseThis enzyme joins digest fragments to form recombinant molecules:16
2901126991recombinantDNA produced by hybridization, which is pieced together from different sources is:17
2901129176flexibleWhile blunt ends are more difficult to join chemically, they are also more ____ in their use.18
2901130460bacterial plasmidLarge amounts of a desired DNA sequence or protein can be produced by ligating it into a(n) __________ and allowing the host cell to replicate it.19
2901180717restriction mapA(n) ______ can be produced by subjecting a plasmid to several restriction enzymes and then comparing the sizes of the fragments produced.20
2901181273electrophoresisThis is a method of separating DNA fragments:21
2901181706size, chargeElectrophoresis separates fragments by ____ and ____.22
2901182475point mutations on restriction sitesRestriction digest can be used in the clinical lab to detect:23
2901187654electrophoresis, transfer to membraneBlot tests consist of two parts:24
2901184447membraneIn a blot test, DNA is separated by electrophoresis and then wicked onto a(n):25
2901186026probeIn molecular biology, the term _____ refers to a sequence of ss-DNA which is labeled with an enzyme, luminescent chemical, or radioisotope.26
2901187119hybridizeIn a blot test, labeled probes _____ with their complimentary strand, which allows the bands to be visualized.27
2901189256southern blotThis test involves electrophoresis, blotting, and labeling to detect DNA:28
2901191272northern blotThis test involves electrophoresis, blotting, and labeling to detect RNA:29
2901192268western blotThis test involves electrophoresis, blotting, and labeling to detect protein:30

DNA Restriction Enzyme Mapping Flashcards

Terms : Hide Images
704440075Molecular biology arose from the confluence of which 7 disciplines?1. genetics 2. physical chemistry 3. x-ray crystallography 4. biochemistry 5. microbiology 6. bacteriology 7. virology0
704440076When were restriction enzymes discovered?-in the 1950s1
704440077What is a restriction enzyme?-an enzyme that cuts DNA at specific recognition nucleotide sequences known as restriction sites2
704440078What is a bacteriophage?-a virus that infects and replicates within bacteria -kills bacteria in the process3
704440079What two important discoveries occurred at the University of Geneva in 1962?-bacteria that are resistant to bacteriophages possess an enzyme system that could selectively recognize and destroy foreign phage DNA within the bacterial membrane -resistant bacteria could modify their own chromosomal DNA to prevent self-destruction4
704440080Later, extracts were made from E. coli that were found to efficiently cleave the DNA of bacteriophages. What did these extracts contain?-non-restriction endonucleases5
704440081What are non-restriction endonucleases?-enzymes that cut internal regions of DNA of an invading bacteriophage and at the same time protected the DNA of the bacterial host -->these enzymes can both cut the phage DNA and also protect bacterial DNA by modifying it6
704440082Why weren't these initial extracts of any use?-because the cutting activity of these enzymes was non-specific7
704440083What new type of bacterial endonuclease was discovered at Johns Hopkins in 1970?-a bacterial endonuclease that could cut at a specific site within the phage DNA, while at the same time protecting the same site on the bacterial host's DNA8
704440084How is the protection of the host's DNA site accomplished?-through the addition of a methyl group to the site9
704440085From what type of bacterium was this new type of bacterial endonuclease isolated?-the bacterium haemophilus influenzae10
704440086What was this enzyme called?-HindII11
704440087How does it cleave DNA?-predictably -cutting within a specific sequence of base pairs, usually only 4 to 8 base pairs in length12
704440088How can the fragments that result from cutting DNA be separated?-by size in an electrical field13
704440089How is it then possible to create a "map" of the restriction enzyme sites?-by finding the size of each fragment14
704440090Is DNA acidic or basic?-acidic15
704440091Why?-due to the presence of the phosphate groups16
704440092Can DNA be made to be negatively or positively charged?-negatively17
704440093How?-when DNA is surrounded by a slightly basic buffer solution, the hydrogens of the phosphate groups can be removed -this leaves negatively charged phosphate groups18
704440094Why is this important to the process of gel electrophoresis?-when placed in an electric field, DNA molecules are attracted to the positive pole19
704440095What is the positive pole referred to as?-the anode20
704440096What is the negative pole referred to as?-the cathode21
704440097During electrophoresis, how do DNA fragments sort within the gel?-by size22
704440098Why?-the longer pieces will encounter more frictional forces than the smaller pieces, meaning that the longer pieces will not be able to travel as far23
704440099Using this information, what can we infer?-that the distance traveled by each piece is inversely proportional to its molecular weight/number of base pairs in the fragment24
704440100What is electrophoresis gel composed of?-cross-linked polymer mesh25
704440101How is it made?-by dissolving agarose (made from seaweed) in water26
704440102How can DNA fragments in different size ranges be separated?-by adjusting the agarose concentration27
704440103What do low agarose concentrations produce?-a loose gel that separates large fragments28
704440104What do high agarose concentration s produce?-a stiff gel that resolves small fragments29
704440105What exact type of gel is used in our experiment?-a 1% gel30
704440106This gel can effectively separate DNA fragments of what size?-those between 100 and 5000 nucleotides31
704440107What is a plasmid?-a small, circular, double-stranded DNA molecule that is found in bacteria -physically separate from chromosomal DNA32
704440108What is the name of the first plasmid to be used routinely in recombinant DNA experiments? This is also the plasmid we will be using in our experiment.-pBR32233
704440109What was this plasmid originally derived from?-E.coli34
704440110It was then artificially constructed to have which two antibiotic resistance genes?-tet -amp35
704440111What do these confer resistance to?-tetracycline -ampicillin36
704440112In this experiment, what are we concerned with?-finding the cutting sites for the three restriction enzymes37
704440113What does each restriction enzyme recognize?-a different set of base paris on which to act38
704440114What is one reason why restriction enzyme mapping is so important?-finding restriction enzyme sites is the first step in characterizing a DNA fragment that has been ligated onto a vector/plasmid39
704440115This can then provide information for what?-DNA sequencing, gene localization, and site-directed mutagenesis40
782827731What did molecular biology borrow from the physical sciences?-the rigorous use of model systems41
782827732What was the conclusion drawn from the observation of the resistance of some bacteria to invading bacteriophages?-resistance must be a property of the bacterial cell itself42
782827733What characteristic of DNA does gel electrophoresis take advantage of?-the fact that, as an organic acid, DNA can be made to be negatively charged43
782827734What is the distance traveled by a DNA fragment in agarose gel inversely proportional to?-molecular weight/the number of base pairs in each fragment -->smaller restriction fragments will migrate relatively far from the origin compared with the larger fragments44
782827735What four restriction enzymes were used in our experiment?-EcoR1 -HincII -PvuII -Hinc III45
782827736What was Hinc III used for?-to make the ladder46
782937728What type of DNA was used to make the ladder in this experiment?-lambda DNA47
782937729What are the three basic components of the loading dye in the experiment?-blue pigment -violet pigment -glycerol48
782937730What is the role of glycerol in the experiment?-the glycerol is heavier than the running buffer solution -as a result, it helps to weigh down the restriction digests within the wells49
782937731What is the function of the pigment in the experiment?-the pigments tell us where our restriction digests are within the agarose gel so that we can turn off the electric current before our fragments run off the end of the plate50
782937732How many different buffers did we use in this experiment?-three51
782937733Why did we add buffer to every restriction digest tube?-the addition of buffer helps to limit changes to the pH of the solution -enzymes function at a very specific pH -thus, the addition of buffer helps to ensure that our enzymes remain functional52
782937734Why did we add buffer when we cast our electrophoresis gel? Why not just use water?-the buffer has a pH of 8, and is therefore able to remove hydrogen atoms from the phosphate groups within DNA -this leaves the DNA negatively charged and capable of moving toward the anode within the electric field -we cannot use pure water because it has a pH of 7 -therefore, it is unable to remove hydrogen atoms from the phosphate groups of DNA53
782937735Why is ethidium bromide (EtBr) added to the agarose gel?-to stain DNA54
782937736Why is EtBr considered to be a hazardous material?-ethidium cation unwinds DNA, creating openings where it can insert itself into the strand -these structural changes can result in functional modifications, including the inhibition of transcription, replication, and repair processes -as a result, EtBr is a suspected carcinogen, mutagen, and teratogen55
782937737What is a teratogen?-an agent that causes the production of physical defects in the developing embryo.56
782937738How does EtBr stain DNA?-EtBr is a planar molecule -as a result, it is capable of unwinding DNA and creating openings where it can insert itself into the strand -this process is known as intercalation -after intercalation occurs, exposure to UV light causes the DNA to fluoresce with an orange color, thus allowing it to be viewed within an agarose gel57
782937739Why did we use a running buffer in the electrophoresis chamber? Why didn't we just use water?-buffer provides ions that are capable of carrying a current -buffer offers a path of least resistance -->water is not capable of generating a current, water offers a path of greater resistance58
782937740In order to run our electrophoresis chambers, why did we cover the gels with just a little buffer; why not use quite a lot?-if we used a large amount of buffer, the current would pass through the buffer itself--not the agarose gel -a thin layer helps to ensure that the current runs through the agarose gel, which results in separation of the DNA fragments59
782937741How do the pigments in the loading dye tell you when to stop the current?-one of the pigments travels at the same rate as the smallest fragment, and one travels at the same rate as the largest fragment60
782937742How long did we electrophorese the digests for?-until the leading pigment ran down the gel to between the numbers four and five61
782937743What would happen if we ran the gel with the electrodes reversed?-if the electrodes were reversed, then the electric field would be reversed as well -the DNA would no longer migrate to the bottom of the gel; instead, it would run out of the top of the gel into the buffer solution -the sample would most likely be lost62
782937744What causes smearing within the gels?-the production of very small fragments that are similar in length -because of their similarity in length, they appear very close together within a gel, and may be indistinguishable63
782937745What causes smearing?-contamination -incorrect buffer concentration -too much running buffer -DNA run too fast or too slow -gel not the right thickness64
782937746What are two reasons that extra bands may appear in some of the lanes?1. small amounts of DNA might not have been completely digested by the restriction enzymes; as a result, these bands may be longer or shorter than the brightest bands within the lane 2. a sample may have spilled or leaked over from one well into a neighbor well65
782937747What organism does "EcoR1" come from?-E. coli66
782937748What organism does HincII come from?-Haemophilus influenzae67
782937749What organism does PvuII come from?-Proteus vulgaris68
782937750Do enzymes work best at a high or low heat?-high heat69
782937751What is agarose made from?-seaweed70
782937752What equation describes the relationship between the distance traveled by a piece of DNA and the number of base pairs?distance traveled ∝ 1/log of base pairs71
782956115When performing digest problems, what does "bright" indicate?-the presence of two pieces of DNA of the same length72
782956116What does "very bright" indicate?-the presence of three pieces of DNA of the same length73
782956117What does "very very bright" indicate?-the presence of four pieces of DNA of the same length74
783066081When drawing a restriction enzyme map, what should always be included at the center of the plasmid?-the plasmid name and size (in base pairs)75

Myers Ap Psych: Chapter 5 (Sensation) Flashcards

Terms : Hide Images
3093378248SensationThe process by which our sensory receptors and nervous system receive and represent stimulus energies from our environment.0
3093382233PerceptionThe process of organizing and interpreting sensory information, enabling us to recognize meaningful objects and events.1
3093459271Bottom-up processingAnalysis that begins with the sensory receptors and works up to the brain's integration of sensory information.2
3093462870Top-down processingInformation processing guided by higher level mental processes, as when we construct perceptions by drawing on our experiences and expectations.3
3093476850Absolute thresholdThe minimum stimulation needed to detect a particular stimulus 50 percent of the time.4
3093480454Signal Detection TheoryA theory predicting how and when we detect the presence of a faint stimulus ("signal") amid background stimulation ("noise"). Detection depends partly on a person's experience, expectations, motivation, and level of fatigue.5
3093493485Difference thresholdThe minimum difference between two stimuli required for detection 50 percent of the time. (Just noticeable difference)6
3093497126SubliminalBelow one's absolute threshold for conscious awareness (Detected less than 50% of the time)7
3093503797Weber's LawThe principle that, to be perceived as different, two stimuli must differ by a constant minimum percentage (Light- 8%, Weight-2%, Tones-0.3% frequency change). The larger amount of stimulus present the large change needed to detect the change.8
3093528970Sensory adaptationDiminished sensitivity as a consequence of constant stimulation.9
3093538476TransductionConversion of one form of energy into another. Transforming stimulus energies (sights, sounds, smells) into neural impulses our brains can interpret10
3093550859Wavelength (HUE)Distance from one peak of one light/sound wave to the peak of the next. Determines the energy's HUE (color we see)11
3093562951Intensity (AMPLITUDE)Amount of energy in light waves (determined by AMPLITUDE - height of the wave) influences the brightness of the color we perceive.12
3093573188PupilAdjustable opening in the center of the eye through which light enters.13
3093575819IrisRing of muscle tissue around the pupil that controls the size of the pupil opening (Determines the color of your eye)14
3093580654LensTransparent structure behind the pupil that changes shape to focus the image on the retina15
3093582513RetinaLight sensitive inner surface of the eye, containing receptor rods and cones plus layers of neurons that begin the process of visual information.16
3093588124RodsRetinal receptors that detect black, white, and gray (Peripheral and low light vision).17
3093591250ConesRetinal receptor cells that are concentrated near the center of the retina. Detect colors in daylight and well lit conditions.18
3093598971FoveaCentral focal point in the retina (location of the eye's cones).19
3093604292Optic Nerve (BLIND SPOT)Nerve that carries neural impulses from the eye to the brain (creating a BLIND SPOT because there are no receptor cells there)20
3093610995Feature detectorsNerve cells in the brain that respond to specific features of the stimulus, such as shape, angle, or movement.21
3093620659Parallel ProcessingProcessing several aspects of a problem at the same time (including color, depth, movement, and form)22
3093630732Young-Helmholtz Trichromatic (three color) TheoryThe retina contains three different color receptors (red, green, blue) - when stimulated in combination can produce the perception of any color.23
3093637619Opponent-Process TheoryOpposing retinal processes (red-green, yellow-blue, white-black) that enable vision.24
3093661818PitchTone's experienced highness or lowness (depends on the frequency - length of the wave)25
3093670201Middle EarThree tiny bones (hammer/malleus, anvil/incus, & stirrup/stapes) that concentrate the vibrations of the eardrum on the cochlea's oval window.26
3093688504CochleaSnail shaped fluid filled tube of the inner ear that is lines with tiny hair cells. Sound waves trigger nerve impulses through these hairs.27
3093718462Conduction hearing lossCaused by damage to the mechanical system (bones in middle ear) that conducts sound waves to the cochlea.28
3093722979Sensorineural hearing loss (Nerve Deafness)Caused by damage to the cochlea's receptors (hair cells) or the auditory nerve.29
3093739846KinesthesisThe system for sensing the position and movement of individual body parts.30
3093742252Vestibular SenseSense of body movement and position, including the sense of balance (sense of equilibrium located in inner ear).31

AP Psych Brain Vocab Flashcards

Functions of the major parts of brain

Terms : Hide Images
238659646BrainstemRelays messages between spinal cord and the brain, from brainstem cranial nerves to cerebrum. Helps control heart rate, breathing rate, blood pressure. Involved with hearing, taste, and other senses0
238659647CerebellumProcess center involved with coordination of muscular movements, balance, precision, timing, body positions. Processes sensory information used by the motor systems1
238659648Cerebral CortexInvolved with most conscious activities for living2
238659649Frontal lobeinvolved with motor control of voluntary movements, reasoning, & control of emotional expressions and moral behavior3
238659650Parietal lobeInvolved with general senses, taste4
238659651Temporal lobeInvolved with hearing, equilibrium, emotion, and memory5
238659652Occipital lobeOrganized for vision and associated forms of expression6
238659653Limbic lobeInvolved with emotions, behavioral expressions, recent memory, and smell7
238659654CerebrumControls voluntary movements, coordinates mental activity, center for all conscious living8
238659655Corpus callosumConnects cerebral hemispheres, relays sensory information between them. Allows left and right hemispheres to share information, and unifies attention9
238659656HypothalamusHighest integrating center for autonomic nervous system. Controls most of the endocrine system through its relationship with the pituitary gland. 5Fs10
238659657Medulla OblongataControls involuntary body functions not under conscious control - heartbeat, respiration, blood pressure, etc.11
238659658Midbraininvolved with visual reflexes, movement of eyes, focusing of lens, dilation of pupils12
238659659PonsControls respiratory functions and regulates dreaming13
238659660Reticular FormationRegulation of arousal, and involves respiratory and cardiovascular centers14
238659661ThalamusSensory switchboard; Intermediate relay structure and processing center for all sensory information except smell.15

Restriction Enzymes Flashcards

Terms : Hide Images
3540190238What is the general function of restriction enzymes? How do they function in bacteria?- to recognize specific dsDNA sequences and cleave them via hydrolysis - in bacteria, restriction enzymes protect the cell from harmful foreign/viral DNA (there are 10x bacteriophages: bacteria, many have dsDNA).0
3540246463What is the DNA sequence recognized by restriction enzymes called?- cognate DNA.1
3540196994Where do restriction enzymes cleave?- depends on their type. - type II (the type we focused on) cleave within their recognition site. - other types cut outside of the recognition site.2
3540236917What are the specificity requirements of restriction enzymes?- must cut foreign DNA - must not cut host DNA3
3540483791Who were the scientists to isolate and characterize the first restriction enzyme? In what year?- Hamilton Smith and Kent Wilcox - 19704
3540497200When were restriction enzymes applied to gene editing? Who was the first to do this?- 1972 - Herb Boyer and Stan Cohen - made the first recombinant DNA using restriction enzymes, eventually obtained that 1st patent and formed Genetech5
3540511909How do restriction enzymes selectively degrade viral DNA without degrading host DNA?- every restriction enzyme has a specific recognition sequence/ cognate DNA - every restriction enzyme has a corresponding/matching methylase that recognizes the same gene sequence - methylase methylates the host's cognate DNA sequence - this blocks the restriction enzyme from cleaving host DNA6
3540567055There is a loop hole in the functional pairing of restriction endonuclease + methylase to protect host DNA from being degraded. What is the loop hole? How does the bacteria compensate for it? Is there a trade-off?- cognate DNA cannot be methylated during replication - compensated by limiting the speed of the restriction enzyme - trade off is that the enzyme needs to act fast enough to protect bacterial DNA from viral DNA, but not too fast that it will cleave cognate bacterial DNA during replication.7
3540588217Give the recognition sequence of EcoRV- 5' GATATC 3'8
3540604883Describe the relationship between restriction endonuclease specificity vs efficiency.- specificity of a restriction enzyme takes place as the level of binding - Ex., EcoRV's recognition sequence is 6 bp long; there are 4^6 possible sequences in that space - this means that, in order to be truly specific, it needs to recognize/cleave the cognate sequence 4^6 times better than non-specific sites.9
3540660084What reaction do restriction endonucleases catalyze? What are the substrates, what are the products?- catalyze the cleave of DNA's phosphodiester backbone via hydrolysis - substrates = water and DNA's phosphodiester backbone - products = 3' OH and 5' phosphoryl group10
3540686035Describe the two possible nucleophilic attack mechanisms that restriction endonucleases might use to cleave DNA's phosphodiester backbone.-1. covalent intermediate. two-step mechanism; original stereochemistry is maintained through 2x inversions -2. direct hydrolysis. one-step mechanism; pentacoordinate transition state formed with trigonal bipyramidal geometry centered at the phosphorous atom. stereochemical inversion.11
3540741789What are the main differences between the two types of nucleophilic attack? Where is each type observed?- covalent intermediate (1) maintains original stereochemistry. - analogous to chymotrypsin's mechanism - direct hydrolysis (2) inverts the original stereochemistry. - analogous to aspartyl- and metallo- proteases.12
3540771601How was it determined what type of nucleophilic attack mechanism restriction endonucleases use?- looked at the stereochemistry - stereochemistry hard to look at since the phosphoryl group has two oxygens on it (ie, substrate is not a chiral centre). - introduced sulphur on to the phosphory (phosphorthioate bond); making it a stereocenter - used labelled water (with O18) to follow stereochemistry after the nucleophilic attack.13
3540805212What type of stereochemistry was observed after the restriction endonuclease nucleophilic attack? What did this indicate?- inverted - single-step mechanism14
3541736134How do divalent cations contribute to restriction endonuclease activity? Which divalent cation is most common?- function is similar to Zn2+ in carbonic anhydrase (interacts with water to lower its pKa and generate a nucleophile) - Mg2+ is most common in restriction enzymes15
3541752337What technique is used to identify the presence/ location of divalent cation binding sites in proteins? What are the limitations of this technique?- grow a crystal of the enzyme-substrate complex in the presence of the divalent cation - problem is that, to grow a crystal, enzymes must be homogenous/ all in the same state (enzymes change states/ catalyze reactions really fast, hard to slow them down enough to be homogenous) - can also grow crystals using a mutant form of your enzyme of interest that has less activity - could substitute in a different divalent cation.16
3541774019How many divalent metal ion binding sites are present in restriction enzymes? In EcoRV, specifically?- up to three sites; at least one is always occupied - EcoRV has two sites17
3541780336How is the divalent cation coordinated in restriction endonucleases?- four positions: - 1. 2x aspartates - 2. one of the oxygen atoms on a phosphoryl group - 3. water18
3541791879Describe the characteristics of the recognition sites that restriction endonucleases bind to.- sites contain inverted repeats with two-fold rotational symmetry - sites are palindromic19
3541802307How is the symmetry of binding sites mirrored in the structure of restriction enzymes?- dimers - also have two-fold rotational symmetry - this facilitates the interaction of the enzyme with the palindromic sequence (alignment of two-fold axis)20
3541811548Describe the key H-bond interactions between the restriction enzymes and the DNA.- enzyme does not h-bond to the cleavage site; h-bonds to the residues flanking the cleavage site21
3541828808Cognate and non-cognate DNA bind to the active site differently. How is their binding different?- both cognate and non-cognate DNA form extensive hydrogen bond interactions with the restriction enzyme - hydrogen bonding to the cognate DNA is more extensive, and causes the DNA to bend up and into position for the two Mg2+ to activate H2O and cleave - the distortion of the cognate DNA generates an entropic penalty - which is satisfied by the extra hydrogen bonds the cognate DNA forms that the non-cognate cannot.22
3541892610How does methylation protect host DNA?- adds steric hinderance - prevents hydrogen bonding interactions necessary to promote DNA in its bent/ distorted shape that's necessary for cleavage catalysis to occur.23
3541901602Describe the structural similarities and difference of restriction endonucleases present within the same class, and the same organism.- overall structures are not conserved and vary quite a bit outside of the active site - the catalytic core is highly conserved.24
3541907722How were restriction enzymes used in genetic engineering?- when restriction enzyme cuts, it doesn't make perfectly neat 'blunt' ends, it makes sticky ends with an overhang - get a plasmid with a restriction site in it, and treat it with the restriction enzyme. - so, what you do is cut your YGI with the same sticky ends that the restriction enzyme makes, and then YGI gets incorporated. - treat it with a ligase to seal up the nicks, and bam. transgene.25
3541928883What does RFLP stand for? How is it applied?- RFLP = restriction length polymorphism. - due to insertions and deletions in people, the space/ amount of DNA between restriction sites is not the same across individuals - restriction enzyme chops up DNA into slightly different lengths/ cut patterns - amplify the RFLPs and run them through a gel, bam. early forensics/ paternity testing.26
3541944400What do you call the phosphodiester bond about to be cut by the restriction endonuclease?- scissile bond27
3541946934Why is two-fold rotational symmetry necessary in the restriction enzyme?- for cleaving both strands of DNA.28
3542130344Describe the mechanism of the CRISPR-Cas9 system.- CRISPR-Cas9 has two main parts: guide RNA and the Cas9 endonuclease. - bacteriophage inserts dsDNA into host cell - when foreign dsDNA comes into cell, cell looks for PAM sequence (protospacer adjacent motif) that gets cut out + directly inserted into the bacterial genome @ CRISPR loci - CRISPR loci = repeat sequences, foreign DNA gets inserted between them. (CRISPR array) - can have up to 100x repeats - physically cuts out the viral DNA and stuck it into its own genome. - when RNA transcribed from that, makes primary transcript, makes tracer/guide RNA. - tracer RNA gets incorporated into Cas9 complex - tracer RNA base pairs directly with foreign DNA - Cas9 cuts foreign DNA, disabling it via improper repair29
3542032667How can CRISPR-Cas9 technology be used?- double strand break can be utilized two ways: - 1. non-homologous end joining (generates a mutation in YGI) - 2. template-directed repair/ homologous end jointing (insert YGI into the genome).30
3542141237How many types of CRISPR systems are there? Which type is Cas9?- three different CRISPR systems - CRISPR-Cas9 is a type II system31
3542152536Why can CRISPR be used to target genes with intense specificity/accuracy?- the longer you make the guide RNA, the more specific it'll be - target one part of the genome specifically32

The American Pageant 14th Edition: Chapters 1-5 Flashcards

Chapter 1-5 vocabulary

Terms : Hide Images
1704601420Canadian Shieldgeological shape of North America; 10 million years ago; held the northeast corner of North America in place; the first part of North America to come above sea level.0
1704601421IncasThe Incas were a Native American Empire who lived in Peru. Their capital was Cuzco. They had a civilization with elaborate network of roads and bridges linking their empire.1
1704601422AztecsThe Azetcs were a Native American Empire who lived in Mexico. Their capital was Tenochtitlan. They worshipped everything around them especially the sun. Cortes conquered them in 1521.2
1704601423Nation-statesThe modern form of political society that combines centralized government with a high degree of ethnic and cultural unity.3
1704601424CahokiaMississippian settlement near present-day East St. Louis, home to as many as 25,000 Native Americans4
1704601425Three-sister FarmingCorn grew in a stalk providing a trellis for beans, beans grew up the stalk, squash's broad leaves kept the sun off the ground and thus kept the moisture in the soil.5
1704601426MiddlemenIn trading systems, those dealers who operate between the original buyers and the retail merchants who sell to consumers.6
1704601427Caravela small, fast ship with a broad bow7
1704601428PlantationA large-scale agricultural enterprise growing commercial crop and usually employing coerced or slave labor.8
1704601429Columbian ExchangeThe exchange of plants, animals, diseases, and technologies between the Americas and the rest of the world following Columbus's voyages9
1704601430Treaty of TordesillasIn 1494 Spain and Portugal were disputing the lands of the new world, so the Spanish went to the Pope, and he divided the land of South America for them. Spain got the vast majority, the west, and Portugal got the east.10
1704601431ConquistadoresSpanish explorers that invaded Central and South America for it's riches during the 1500's. In doing so they conquered the Incas, Aztecs, and other Native Americans of the area. Eventually they intermarried these tribes.11
1704601432CapitalismAn economic system characterized by private property, generally free trade, and open and accessible markets12
1704601433EncomiendaGrants of Indian laborers made to Spanish conquerors and settlers in Mesoamerica and South America; basis for earliest forms of coerced labor in Spanish colonies.13
1704601434Noche triste"sad night", when the Aztecs attacked Hernán Cortés and his forces in the Aztec capital, Tenochitlán, killing hundreds. Cortés laid siege to the city the following year, precipitating the fall of the Aztec Empire and inaugurating three centuries of Spanish rule14
1704601435MestizosThe Mestizos were the race of people created when the Spanish intermarried with the surviving Indians in Mexico.15
1704601436Battle of AcomaFought between Spaniards under Don Juan de Onate and the Pueblo Indians in present-day New Mexico. Spaniards brutally crushed the Pueblo peoples and established the territory as New Mexico in 1609.16
1704601437Popé's Rebellionrevolt in which Indians took over New Mexico and held control for nearly half a century.17
1704601438Black LegendThe idea developed during North American colonial times that the Spanish utterly destroyed the Indians through slavery and disease while the English did not. It is a false assertion that the Spanish were more evil towards the Native Americans than the English were.18
1704601439Ferdinand of AragonKing of Spain with his marriage to Isabella of Castile and together they brutally expelled the Muslim caliphate of Córdoba from Spain during the Reconquista. They also funded the expeditions of Christopher Columbus.19
1704601440Isabella of CastileQueen of Spain with her marriage to Ferdinand of Aragon and together they brutally expelled the Muslim caliphate of Córdoba from Spain during the Reconquista. They also funded the expeditions of Christopher Columbus.20
1704601441Christopher ColumbusAn Italian navigator who was funded by the Spanish Government to find a passage to the Far East. He is given credit for discovering the "New World," even though at his death he believed he had made it to India. He made four voyages to the "New World." The first sighting of land was on October 12, 1492, and three other journies until the time of his death in 1503.21
1704601442Francisco PizarroNew World conqueror; Spanish conqueror who crushed the Inca civilization in Peru; took gold, silver and enslaved the Incas in 1532.22
1704601443Bartolome de las CasasA Spanish missionary who was appalled by the method of encomienda, calling it "a moral pestilence invented by Satan."23
1704601444Hernan CortesHe was a Spanish explorer who conquered the Native American civilization of the Aztecs in 1519 in what is now Mexico.24
1704601445Malinche (Dona Maria)A female Indian slave who knew Mayan and Nahuatl, the language of the Aztec.25
1704601446MoctezumaAztec chieftan; encountered Cortes and the Spanish and saw that they rode horses; Montezuma assumed that the Spanish were gods. He welcomed them hospitably, but the explorers soon turned on the natives and ruled them for three centuries.26
1704601447Giovani Caboto (John Cabot)(an Italian who sailed for England) touched the coast of the current U.S.27
1704601448Robert de La Sallesailed down the Mississippi River for France claiming the whole region for their King Louis and naming the area "Louisiana" after his king. This started a slew of place-names for that area, from LaSalle, Illinois to "Louisville" and then on down to New Orleans (the American counter of Joan of Arc's famous victory at Orleans).28
1704601449Father Junipero SerraThe Spanish missionary who founded 21 missions in California, in 1769, he founded Mission San Diego, the first of the chain.29
1704601450Protestant ReformationMovement to reform the Catholic Church launched in Germany by Martin Luther. Reformers questioned the authority of the Pope, sought to eliminate the selling of indulgences, and encouraged the translation of the Bible from Latin, which few at the time could read. The reformation was launched in England in the 1530s when King Henry VIII broke with the Roman Catholic Church30
1704601451Roanoke IslandSir Walter Raleigh's failed colonial settlement off the coast of North Carolina31
1704601452Spanish ArmadaSpanish fleet defeated in the English Channel in 1588. The defeat of the Armada marked the beginning of the decline of the Spanish Empire.32
1704601453PrimogenitureLegal principle that the oldest son inherits all family property or land. Landowner's younger sons, forced to seek their fortunes elsewhere, pioneered early exploration and settlement of the Americas33
1704601454Joint-Stock CompanyShort-term partnership between multiple investors to fund a commercial enterprise; such arrangements were used to fund England's early colonial vestures34
1704601455CharterLegal document granted by a government to some group or agency to implement a stated purpose, and spelling out the attending rights and obligations. British colonial charters guaranteed inhabitants all the rights of Englishmen, which helped solidify colonists' ties to Britain during the early years of settlement.35
1704601456JamestownFirst permanent English settlement in North America founded by the Virginia Company36
1704601457First Anglo-Powhatan WarSeries of clashes between the Powhatan Confederacy and English settlers in Virginia. English colonists torched and pillaged Indian villages, applying tactics used in England's campaigns against the Irish.37
1704601458Second Anglo-Powhatan WarLast-ditch effort by the Indians to dislodge Virginia settlements. The resulting peach treaty formally separated white and Indian areas of settlement.38
1704601459Act of TolerationPassed in Maryland, it guaranteed toleration to all Christians but decreed the death penalty for those, like Jews and atheists, who denied the divinity of Jesus Christ. Ensured that Maryland would continue to attract a high proportion of Catholic migrants throughout the colonial period.39
1704601460Barbados Slave CodeFirst formal statute governing the treatment of slaves, which provided for harsh punishments against offending slaves but lacked penalties for the mistreatment of slaves by masters. Similar statutes were adopted by Southern plantation societies on the North American mainland in the 17th and 18th centuries.40
1704601461SquattersFrontier farmers who illegally occupied land owned by others or not yet officially opened for settlement. Many of North Carolina's early settlers were squatters, who contributed to the colony's reputation as being more independent-minded and "democratic" than its neighbors.41
1704601462Tusarora WarBegan with an Indian attack on Newbern, North Carolina. After the Tusaroras were defeated, remaining Indian survivors migrated northward, eventually joining the Iroquois Confederacy as its sixth nation.42
1704601463Yamasee IndiansDefeated by the South Carolinians in the war of 1715-1716. The Yamasee defeat devastated the last of the coastal Indian tribes in the Southern colonies43
1704601464BufferIn politics, a territory between two antagonistic powers, intended to minimize the possibility of conflict between them. In British North America, Georgia was established as a buffer colony between British and Spanish territory.44
1704601465Iroquois ConfederacyBound together five tribes - the Mohawks, Oneidas, Onondagas, Cayugas, and Senecas - in the Mohawk Valley of what is now New York State45
1704601466Henry VIIIBritish monarch in the 1530s who broke ties with the Roman Catholic Church after wanting a divorce with Catherine of Aragon. By doing so, he brought the Protestant Reformation to England.46
1704601467Elizabeth ISometimes called The Virgin Queen, Gloriana, or Good Queen Bess, Elizabeth was the fifth and last monarch of the Tudor dynasty. Made the break with the Roman Catholic Church final47
1704601468Sir Frances DrakeVice Admiral was an English sea captain, privateer, navigator, slaver, and politician of the Elizabethan era. was awarded knighthood in 1581 by Elizabeth I48
1704601469Sir Walter RaleighEnglish aristocrat, writer, poet, soldier, courtier, spy, and explorer. He is also well known for popularizing tobacco in England & led the expedition to North Carolina's Roanoke Island49
1704601470James Isent a charter to the settlers in the New World from the Virginia Company that granted rights to overseas settlers Captain50
1704601471John SmithAdmiral of New England and an English soldier, explorer, and author. Took leadership at Jamestown and saved from execution by Indian women51
1704601472Powhatanalso known as Wahunsenacawh, was the paramount chief of Tsenacommacah, an alliance of Algonquian-speaking Virginia Indians in the Tidewater region of Virginia while English settlers landed at Jamestown in 160752
1704601473PocahontasVirginia Indian notable for her association with the colonial settlement at Jamestown, Virginia. She was the daughter of Powhatan, the paramount chief of a network of tributary tribal nations in the Tidewater region of Virginia.53
1704601474Lord De La Warrwas the Englishman after whom the bay, the river, and, consequently, an American Indian people and U.S. state, all later called "Delaware"54
1704601475John RolfeEarly Virginia colonist who fought in the First Anglo-Powhatan War. Later, he married Pocahontas55
1704601476Lord Baltimorefounder of the Maryland colony, a prominent English Catholic. He embarked upon the venture to reap financial profits and to create a refuge for his fellow Catholics56
1704601477Oliver CromwellRuler of the Commonwealth of England during the Puritan Revolution and beheaded Charles I57
1704601478James Oglethorpefounder of the Georgia Colony who gave Georgia the nickname "The Charity Colony"58
1704601479Hiawathanotable leader of the Iroquois Confederacy before its fall to the Caucasians59
1704601480CalvinismDominant theological credo of the New England Puritans based on the teachings of John Calvin. Calvinists believed in predestination - that only "the elect" were destined for salvation.60
1704601481PredestinationCalvinist doctrine that God has foreordained some people to be saved and some to be damned. Though their fate was irreversible, Calvinists, particularly those who believed they were destined for salvation, sought to lead sanctified lives in order to demonstrate to others that they were in fact members of the "elect."61
1704601482ConversionIntense religious experience that confirmed and individual's place among the "elect", or the "visible saints." Calvinists who experienced conversion were then expected to lead sanctified lives to demonstrate their salvation.62
1704601483PuritansEnglish Protestant reformer who sought to purify the Church of England of Catholic rituals and creeds. Some devout Puritans believed that only "visible saints" should be admitted to church membership63
1704601484SeparatistsSmall group of Puritans who sought to break away entirely from the Church of England; after initially settling in Holland, a number of English Separatists made their way to Plymouth Bay, Massachusetts in 1620.64
1704601485Mayflower CompactAgreement to form a majoritarian government in Plymouth, signed aboard the Mayflower. Created a foundation for self-government in the colony65
1704601486Massachusetts Bay ColonyEstablished by non-separating Puritans, it soon grew to be the largest and most influential of the New England colonies66
1704601487Great MigrationMigration of seventy thousand refugees from England to the North American colonies, primarily New England and the Caribbean. The twenty thousand migrants who came to Massachusetts largely shared a common sense of purpose - to establish a model Christian settlement in the new world.67
1704601488AntinomianismBelief that the elect need not obey the law of either God or man; most notably espoused in the colonies by Anne Hutchinson68
1704601489Fundamental OrdersDrafted by the settlers in the Connecticut River Valley, document was the first "modern constitution: establishing a democratically controlled government. Key features of the document were borrowed for Connecticut's colonial charter and its state constitution. Pequot War: Series of clashes between English settlers and Pequot Indians in the Connecticut River Valley. Ended in the slaughter of the Pequots by the Puritans and their Narragansett Indian allies.69
1704601490English Civil WarArmed conflict between royalists and parlimentarians, resulting in the victory of Pro-Parliament forces and the execution of Charles I70
1704601491Dominion of New EnglandAdministrative union created by royal authority, incorporating all of New England, New York, and East and West Jersey. Placed under the rule of Sir Edmund Andros who curbed popular assemblies, taxed residents without their consent, and strictly enforced the Navigation Laws. Its collapse after the Glorious Revolution in England demonstrated colonial opposition to strict royal control71
1704601492Navigation LawsSeries of laws passed, beginning in 1651, to regulate colonial shipping; the acts provided that only English ships would be allowed to trade in English and colonial ports, and that all goods destined for the colonies would first pass through England.72
1704601493Glorious RevolutionRelatively peaceful overthrow of the unpopular Catholic monarch, James II, replacing him with Dutch-born William III and Mary, daughter of James II. William and Mary accepted increased Parliamentary oversight and new limits on monarchical authority.73
1704601494Salutary NeglectUnofficial policy of relaxed royal control over colonial trade and only weak enforcement of Navigation Laws. Lasted from the Glorious Revolution to the end of the French and Indian War in 176374
1704601495PatroonshipsVast tracts of land along the Hudson River in New Netherlands granted to wealthy promoters in exchange for bringing fifty settlers to the property.75
1704601496Blue LawsAlso known as sumptuary laws, they are designed to restrict personal behavior in accord with a strict code of morality. Blue laws were passed across the colonies, particularly in Puritan New England and Quaker Pennsylvania76
1704601497Martin Lutherleader of the Protestant Reformation in Wittenberg, Germany. Founder of the Lutheran religion.77
1704601498John CalvinJohn Calvin was responsible for founding Calvinism, which was reformed Catholicism. He writes about it in "Institutes of a Christian Religion" published in 1536. He believed God was all knowing and everyone was predestined for heaven or hell.78
1704601499William BradfordA pilgrim that lived in a north colony called Plymouth Rock in 1620. He was chosen governor 30 times. He also conducted experiments of living in the wilderness and wrote about them; well known for "Of Plymouth Plantation."79
1704601500John Winthrop:John Winthrop immigrated from the Mass. Bay Colony in the 1630's to become the first governor and to led a religious experiment. He once said, "we shall be a city on a hill."80
1704601501Anne HutchinsonA religious dissenter whose ideas provoked an intense religious and political crisis in the Massachusetts Bay Colony between 1636 and 1638. She challenged the principles of Massachusetts's religious and political system. Her ideas became known as the heresy of Antinomianism, a belief that Christians are not bound by moral law. She was latter expelled, with her family and followers, and went and settled at Pocasset ( now Portsmouth, R.I.)81
1704601502Roger WilliamsEnglish Protestant theologian who was an early proponent of religious freedom and the separation of church and state. In 1636, he began the colony of Providence Plantation, which provided a refuge for religious minorities.82
1704601503Massasoitwas the sachem, or leader, of the Wampanoag, and "Massasoit" of the Wampanoag Confederacy. The term Massasoit means Great Sachem.83
1704601504Metacom (King Phillip)a war chief or sachem of the Wampanoag Indians and their leader in King Philip's War, a widespread Native American uprising against English colonists in New England.84
1704601505Charles IIRuler of Britain after Oliver Cromwell and brought back the traditional British Monarchy85
1704601506Sir Edmund AndrosHead of the Dominion of New England in 1686, militaristic, disliked by the colonists because of his affiliation with the Church of England, changed many colonial laws and traditions without the consent of the representatives, tried to flee America after England's Glorious Revolution, but was caught and shipped to England86
1704601507William IIIKing of England with his wife, Mary II. Both were from Holland. Reigned after the Glorious Revolution in England.87
1704601508Mary IIQueen of England with her husband, William III. Both were from Holland. Reigned after the Glorious Revolution in England.88
1704601509Henry HudsonDiscovered what today is known as the Hudson River. Sailed for the Dutch even though he was originally from England. He was looking for a northwest passage through North America.89
1704601510Peter StuyvesantA Dutch General; He led a small military expedition in 1664. He was known as "Father Wooden Leg". Lost the New Netherlands to the English. He was governor of New Netherlands90
1704601511Duke of YorkUnpopular Catholic monarch; He was dethroned in 1688 during the Glorious Revolution91
1704601512William PennEnglish Quaker;" Holy Experiment"; persecuted because he was a Quaker; 1681 he got a grant to go over to the New World; area was Pennsylvania; "first American advertising man"; freedom of worship there92
1704601513Indentured Servantsperson who agreed to work for a colonial employer for a specified time in exchange for passage to America.93
1704601514Headright Systemsystem employed in Virginia and Maryland to encourage the importation of servant workers; whoever paid the passage of a laborer received the right to acquire fifty acres of land94
1704601515Bacon's Rebellion1676 Virginian rebellion of frontiersmen (wretched bachelors) sparked by governor Berkeley's refusal to retaliate for a series of brutal Indian attacks on frontier settlements; killed Indians, chased Berkeley from Jamestown, and set fire to Jamestown; plundering and pilfering; crushed by Berkeley with cruelty of haging over twenty rebels; rebellion ignited resentments of landless former servants and pitted the frontiersmen against the gentry of the plantations; caused gentry to seek out African slaves95
1704601516Royal African CompanyEnglish stock-joint company that enjoyed a state-granted monopoly on the colonial slave trade from 1672 until 1698. The supply of slaves to the North American colonies rose sharply once the company lost its monopoly privileges.96
1704601517Middle Passagethe transatlantic sea voyage that brought slaves to the New World; the long and hazardous "middle" segment of a journey that began with a forced march to the African coast and ended with a streak into the American interior97
1704601518New York Slave RevoltUprising of approximately two dozen slaves that resulted in the deaths of nine whites and the brutal execution of twenty-one participating blacks98
1704601519South Carolina Slave Revolt (Stono River)Uprising of more than fifty South Carolina blacks along the Stono River. They tried to reach Spanish Florida but were stopped by South Carolina militia.99
1704601520Congregational ChurchSelf governing Puritan congregations without the hierarchical establishment of the Anglican Church.100
1704601521Jeremiada new form of sermon in the Puritan churches in the mid-seventeenth century; preachers scolded parishioners for their waning piety101
1704601522Half-Way Covenant1662, arrangement in Puritan churches which modified the covenant to admit to baptism the unconverted children of existing members; weakened the distinction between the elect and others; led to widening of church membership; afterwords, women became majority in Puritan churches102
1704601523Salem Witch Trialsthe legal lynching in 1692 of twenty individuals, ninteen of whom were hanged and one of whom was pressed to death; two dogs were also hanged; in Salem, Massachusetts; represented the widening social stratification of New England and the fear of many religious traditionalists that the Puritan heritage was being eclipsed by Yankee commercialism103
1704601524Leisler's Rebellionan ill-starred and bloody insurgence that rocked NYC from 1689 to 1691; fueled by animosity between lordly landholders and aspiring merchants104
1704601525William BerkeleyVirginian governor who disliked wretched bachelors (poor, endebted, discontented, and armed); disliked by wretched bachelors for friendly relations with Indians105
1704601526Nathanial Bacontwenty-nine-year-old planter who led a 1676 rebellion of frontiersmen (wretched bachelors) against Berkeley's friendly relations with Indians; in Virginia; died suddenly of disease106
1704601527Anthony JohnsonSlave in Northampton County, Virginia who bought his freedom and became a slaveholder himself107
1704601528Paxton BoysThey were a group of Scots-Irish men living in the Appalachian hills that wanted protection from Indian attacks. They made an armed march on Philadelphia in 1764. They protested the lenient way that the Quakers treated the Indians. Their ideas started the Regulator Movement in North Carolina.108
1704601529Regulator MovementIt was a movement during the 1760's by western North Carolinians, mainly Scots-Irish, that resented the way that the Eastern part of the state dominated political affairs. They believed that the tax money was being unevenly distributed. Many of its members joined the American Revolutionists.109
1704601530Triangular TradeTriangular trade was a small, profitable trading route started by people in New England who would barter a product to get slaves in Africa, and then sell them to the West Indies in order to get the same cargo of goods that would help in repeating this process. This form of trading was used by New Englanders in conjunction with other countries in the 1750's.110
1704601531Molasses ActA British law passed in 1773 to change a trade pattern in the American colonies by taxing molasses imported into colonies not ruled by Britain. Americans responded to this attempt to damage their international trade by bribing and smuggling. Their protest of this and other laws led to revolution.111
1704601532Arminianisma group within the Church of England who rejected Puritanism and the Calvinist doctrine of predestination in favor of free will and an elaborate liturgy; supported by Charles I112
1704601533Great AwakeningThe Great Awakening was a religious revival held in the 1730's and 1740's to motivate the colonial America. Motivational speakers such as Jonathan Edwards and George Whitefield helped to bring Americans together.113
1704601534Old and New LightsIn the early 1700's, old lights were simply orthodox members of the clergy who believed that the new ways of revivals and emotional preaching were unnecessary. New lights were the more modern- thinking members of the clergy who strongly believed in the Great Awakening. These conflicting opinions changed certain denominations, helped popularize missionary work and assisted in the founding educational centers now known as Ivy League schools.114
1704601535Poor Richard's AlmanacBenjamin Franklin's popular collection of information, parables, and advice115
1704601536Zenger trialHe was jailed for questioning the governor of New York. His case influenced freedom of speech and freedom of press.116
1704601537Royal Coloniescolonies that have a governor and council that were designated by the British crown. The people still chose their legislature Proprietary Colony: was a colony in which one or more individuals, usually land owners, remaining subject to their parent state's sanctions, retained rights that are today regarded as the privilege of the state, and in all cases eventually became so117
1704601538Michel-Guillaume Jean de CrevecoeurFrench settler on America in the 1770's; he posed the question of what "American" is after seeing people in America like he had never seen before. American really became a mixture of many nationalities.118
1704601539Jacobus Arminiusthe founder of the anti-Calvinistic school in Reformed Protestant theology, thereby lending his name to a movement which resisted some of the tenets of Calvinism - Arminianism. The early Dutch followers of Arminius' teaching were also called the Remonstrants, after they issued a document containing five points of disagreement with classic Calvinism, entitled Remonstrantice (1610).119
1704601540Jonathan Edwardsan American theologian and Congregational clergyman, whose sermons stirred the religious revival, called the Great Awakening. He is known for his " Sinners in the Hands of an Angry God " sermon.120
1704601541George Whitfielda great preacher who had recently been an alehouse attendant. Everyone in the colonies loved to hear him preach of love and forgiveness because he had a different style of preaching. This led to new missionary work in the Americas in converting Indians and Africans to Christianity, as well as lessening the importance of the old clergy.121
1704601542John Trumbullcolonial painter who studied and worked in Britain and are considered the great American colonial portrait painters122
1704601543John Singleton Copleya famous Revolutionary era painter, Copley had to travel to England to finish his study of the arts. Only in the Old World could Copley find subjects with the leisure time required to be painted, and the money needed to pay him for it. Although he was an American citizen, he was loyal to England during The Revolution.123
1704601544Phillis WheatlyBorn around 1753, Wheatley was a slave girl who became a poet. At age eight, she was brought to Boston. Although she had no formal education, Wheatley was taken to England at age twenty and published a book of poetry. Wheatley died in 1784.124
1704601545John Peter Zengercolonial printer whose case helped begin freedom of the press125

APUSH Chapter 16- The South and the Slave Controversy Flashcards

Terms : Hide Images
1411348067As a result of the introduction of the cotton gin,slavery was reinvigorated0
1411348068All of the following were true of slavery in the South except thatmost slaves were raised in single unstable parent households1
1411348069In the pre-Civil War South, the most uncommon and least successful form of slave resistance wasarmed insurrection2
1411348070The profitable southern slave systemhobbled the economic development of the region as a whole3
1411348071Many abolitionists turned to political action in 1840 when they backed the presidential candidate of theLiberty Party4
1411348072Those in the north who opposed the abolitionists believed that these opponents of slaverywere creating disorder in America5
1411348073The idea of recolonizing blacks back to Africa wassupported by the black leader Martin Delaney6
1411348074Most white southerners weresubsistence farmers7
1411348075Plantation agriculture was wasteful largely becauseits excessive cultivation of cotton despoiled good land8
1411348076Regarding work assignments, slaves weregenerally spared dangerous work9
1411348077Most slaves were raisedin stable two-parent households10
1411348078William Lloyd Garrison pledged his dedication tothe immediate abolition of slavery in the south11
1411348079The voice of white southern abolitionism fell silent at the beginning of the1830s12
1411348080All of the following were true of the American economy under Cotton Kingdom exceptthe South reaped all the profits from the cotton trade13
1411348081By 1860, life for slaves was most difficult in thenewer states of Alabama, Mississippi, and Louisiana14
1411348082As a result of white southerners' brutal treatment of their slaves and their fear of potential slave rebellions, the southdeveloped a theory of biological racial superiority15
1411348083The following abolitionists are matched with their role in the movement.Wendell Phillips- abolitionist golden trumpet Frederick Douglass- black abolitionist Elijah P. Lovejoy- abolitionist martyr William Lloyd Garrison- abolitionist newspaper publisher16
1411348084In arguing for the continuation of slavery after 1830, southernersplaced themselves in opposition to much of the rest of the western world17
1411348085By 1860, slaves were concentrated in the "black belt" located in theDeep South states of Georgia, Alabama, Mississippi, and Louisiana18
1411348086As a substitute for the wage-incentive system, slaveowners most often used thewhip as a motivator19
1411348087The following events are in chronological order.1. American Colonization Society 2. American Anti-Slavery Society 3. Liberty Party20
1411348088Northern attitudes toward free blacks can best be described asdisliking the individuals but liking the race21
1411348089The most pro-Union of the white southerners weremountain whites22
1411348090Slaves fought the system of slavery in all of the following ways except byrefusing to get an education23
1411348091For free blacks living in the north,discrimination was common24

Chapter 6 - AP Biology (Campbell/Reece Biology, Eighth Edition) Flashcards

Vocabulary

Terms : Hide Images
1303339636Light Microscope (LM)An optical instrument with lenses that refract (bend) visible light to magnify images of specimens.0
1303339637OrganelleAny of several membrane-enclosed structures with specialized functions, suspended in the cytosol of eukaryotic cells.1
1303339638Electron Microscope (EM)A microscope that uses magnets to focus an electron beam on or through a specimen, resulting in resolving power a thousandfold greater than that of a light microscope. A transmission electron microscope (TEM) is used to study the internal structure of thin sections of cells. A scanning electron microscope (SEM) is used to study the fine details of cell surfaces.2
1303339639Scanning Electron Microscope (SEM)A microscope that uses an electron beam to scan the surface of a sample to study details of its topography.3
1303339640Transmission Electron Microscope (TEM)A microscope that passes an electron beam through very thin sections and is primarily used to study the internal ultrastructure of cells.4
1303339641Cell FractionationThe disruption of a cell and separation of its parts by centrifugation.5
1303339642CytosolThe semifluid portion of the cytoplasm.6
1303339643Eukaryotic CellA type of cell with a membrane-enclosed nucleus and membrane-enclosed organelles. Organisms with __________ _____ (protists, plants, fungi, and animals) are called eukaryotes.7
1303339644Prokaryotic CellA type of cell lacking a membrane-enclosed nucleus and membrane-enclosed organelles. Organisms with ___________ _____ (bacteria and archaea) are called prokaryotes.8
1303339645NucleoidA dense region of DNA in a prokaryotic cell.9
1303339646CytoplasmThe contents of the cell, exclusive of the nucleus and bounded by the plasma membrane.10
1303339647Plasma MembraneThe membrane at the boundary of every cell that acts as a selective barrier, regulating the cell's chemical composition.11
1303339648Nucleus(1) An atom's central core, containing protons and neutrons. (2) The chromosome-containing organelle of a eukaryotic cell. (3) A cluster of neurons.12
1303339649Nuclear EnvelopeThe double membrane in a eukaryotic cell that encloses the nucleus, separating it from the cytoplasm.13
1303339650Nuclear LaminaA netlike array of protein filaments lining the inner surface of the nuclear envelope; it helps maintain the shape of the nucleus.14
1303339651ChromosomeA cellular structure carrying genetic material, found in the nucleus of eukaryotic cells. Each chromosome consists of one very long DNA molecule and associated proteins. (A bacterial chromosome usually consists of a single circular DNA molecule and associated proteins. It is found in the nucleoid region, which is not membrane bounded.)15
1303339652ChromatinThe complex of DNA and proteins that makes up a eukaryotic chromosome. When the cell is not dividing, chromatin exists in its dispersed form, as a mass of very long, thin fibers that are not visible with a light microscope.16
1303339653NucleolusA specialized structure in the nucleus, consisting of chromatin regions containing ribosomal RNA genes along with ribosomal proteins imported from the cytoplasmic site rRNA synthesis and ribosomal subunit assembly.17
1303339654RibosomeA complex of rRNA and protein molecules that functions as a site of protein synthesis in the cytoplasm; consists of a large and a small subunit. In eukaryotic cells, each subunit is assembled in the nucleolus.18
1303339655Endomembrane SystemThe collection of membranes inside and around a eukaryotic cell, related either through direct physical contact or by the transfer of membranous vesicles; includes the smooth and rough endoplasmic reticulum, the Golgi apparatus, lysosomes, and vacuoles.19
1303339656VesicleA sac made of membrane in the cytoplasm.20
1303339657Endoplasmic Reticulum (ER)An extensive membranous network in eukaryotic cells, continuous with the outer nuclear membrane and composed of ribosome-studded (rough) and ribosome-free (smooth) regions.21
1303339658Smooth ERThat portion of the endoplasmic reticulum that is free of ribosomes.22
1303339659Rough ERThat portion of the endoplasmic reticulum studded with ribosomes.23
1303339660GlycoproteinA protein with one or more carbohydrates covalently attached to it.24
1303339661Transport VesicleA tiny membranous sac in a cell's cytoplasm carrying molecules produced by the cell.25
1303339662Golgi ApparatusAn organelle in eukaryotic cells consisting of stacks of flat membranous sacs that modify, store, and route products of the endoplasmic reticulum and synthesize some products, notably non-cellulose carbohydrates.26
1303339663LysosomeA membrane-enclosed sac of hydrolytic enzymes found in the cytoplasm of animal cells and some protists.27
1303339664PhagocytosisA type of endocytosis in which large particulate substances are taken up by a cell. It is carried out by some protists and by certain immune cells of animals (in mammals, mainly macrophages, neutrophils, and dendritic cells.)28
1303339665Food VacuoleA membranous sac formed by phagocytosis of microorganisms of particles to be used as food by the cell.29
1303339666Contractile VacuoleA membranous sac that helps move excess water out of certain fresh-water protists.30
1303339667Central VacuoleA membranous sac in a mature plant cell with diverse roles in reproduction, growth, and development.31
1303339668MitochondrionAn organelle in eukaryotic cells that serve as the site of cellular respiration.32
1303339669ChloroplastAn organelle found in plants and photosynthetic protists that absorbs sunlight and uses it to drive the synthesis of organic compounds from carbon dioxide and water.33
1303339670PeroxisomeAn organelle containing enzymes that transfer hydrogen (H₂) from various substrates to oxygen (O₂), producing and then degrading hydrogen peroxide (H₂O₂).34
1303339671CristaAn infolding of the inner membrane of a mitochondrion that houses electron transport chains and molecules of the enzyme catalyzing the synthesis of ATP (ATP synthase).35
1303339672Mitochondrial MatrixThe compartment of the mitochondrion enclosed by the inner membrane and containing enzymes and substrates for the citric acid cycle.36
1303339673PlastidOne of a family of closely related organelles that includes chloroplasts, chromoplasts, and amyloplasts (leucoplasts). Plastids are found in cells of photosynthetic organisms.37
1303339674ThylakoidA flattened membranous sac inside a chloroplast. Thylakoids exist in an interconnected system in the chloroplast and contain the molecular "machinery" used to convert light energy to chemical energy.38
1303339675GranumA stack of membrane-bounded thylakoids in the chloroplast. Grana function in the light reactions of photosyntehsis.39
1303339676StromaWithin the chloroplast, the dense fluid of the chloroplast surrounding the thylakoid membrane; involved in the synthesis of organic molecules from carbon dioxide and water.40
1303339677CytoskeletonA network of microtubules, microfilaments, and intermediate filaments that branch throughout the cytoplasm and serve a variety of mechanical, transport, and signaling functions.41
1303339678Motor ProteinA protein that interacts with cytoskeletal elements and other cell components, producing movement of the whole cell or parts of the cell.42
1303339679MicrotubuleA hollow rod composed of tubulin proteins that make up part of the cytoskeleton in all eukaryotic cells and is found in cilia and flagella.43
1303339680CentrosomeStructure present in the cytoplasm of animal cells, important during cell division; functions as a microtubule-organizing center. A centrosome has two centrioles.44
1303339681CentrioleA structure in the centrosome of an animal cell composed of a cylinder of microtubule triplets arranged in a 9 + 0 pattern. A centrosome has a pair of centrioles.45
1303339682FlagellumA long cellular appendage specialized for locomotion. Like motile cilia, eukaryotic flagella have a core with nine outer doublet microtubules and two inner single microtubules ensheathed in an extension of the plasma membrane. Prokaryotic flagella have a different structure.46
1303339683CiliumA short cellular appendage containing microtubules. a motile cilium is specialized for locomotion and is formed from a core of nine outer doublet microtubules and two inner single microtubules ( the "9 + 2" arrangement) ensheathed in an extension of the plasma membrane. A primary cilium is usually nonmotile and plays a sensory and signaling role; it lacks the two inner microtubules (the "9 + 0" arragement).47
1303339684Basal BodyA eukaryotic cell structure consisting of a 9 + 0 arrangement of microtubule triplets. The _____ ____ may organize the microtubule assembly of a cilium or flagellum and is structurally very similar to a centriole.48
1303339685DyneinIn cilia and flagella, a large contractile protein extending from one microtubule doublet to the adjacent doublet. ATP hydrolysis drives changes in dynein shape that lead to bending of cilia and flagella.49
1303339686MicrofilamentA cable composed of actin proteins in the cytoplasm of almost every eukaryotic cell, making up part of the cytoskeleton and acting alone or with myosin to cause cell contraction; also known as an actin filament.50
1303339687ActinA globular protein that links into chains, two of which twist helically about each other, forming microfilaments (actin filaments) in muscle and other kinds of cells.51
1303339688Cortex(1) the outer region of cytoplasm in a eukaryotic cell, lying just under the plasma membrane, that has a more gel-like consistency than the inner regions, due to the presence of multiple microfilaments. (2) In plants, ground tissue that is between the vascular tissue and dermal tissue in a root or eudicot stem.52
1303339689MyosinA type of protein filament that acts as a motor protein with actin filaments to cause cell contraction.53
1303339690PseudopodiumA cellular extension of amoeboid cells used in moving and feeding.54
1303339691Cytoplasmic StreamingA circular flow of cytoplasm, involving myosin and actin filaments, that speeds the distribution of materials within cells.55
1303339692Intermediate FilamentA component of the cytoskeleton that includes filaments intermediate in size between microtubules and microfilaments.56
1303339693Cell WallA protective layer external to the plasma membrane in the cells of plants, prokaryotes, fungi, and some protists. Polysaccharides such as cellulose (in plants and some protists), chitin (in fungi), and peptidoglycan (in bacteria) are an important structural component of ____ _____.57
1303339694Primary Cell WallIn plants, a relatively thin and flexible layer first secreted by a young cell.58
1303339695Middle LamellaIn plants, a thin layer of adhesive extracellular material, primarily pectins, found between the primary walls of adjacent young cells.59
1303339696Secondary Cell WallIn plants, a strong and durable matrix often deposited in several laminated layers for cell protection and support.60
1303339697Extracellular Matrix (ECM)The substance in which animal cells are embedded, consisting of protein and polysaccharides synthesized and secreted by cells.61
1303339698CollagenA glycoprotein in the extracellular matrix of animal cells that forms strong fibers, found extensively in connective tissue and bone; the most abundant protein in the animal kingdom.62
1303339699ProteoglycanA glycoprotein consisting of a small core protein with many carbohydrate chains attache, found in the extracellular matrix of animal cells. A proteoglycan may consist of up to 95% carbohydrate.63
1303339700FibronectinA glycoprotein that helps animal cells attach to the extracellular matrix.64
1303339701IntegrinIn animal cells, a transmembrane receptor protein that interconnects the extracellular matrix and the cytoskeleton.65
1303339702PlasmodesmaAn open channel in the cell wall of a plant through which strands of cytosol connect from an adjacent cell.66

Out of Many Chapter 15 Flashcards

Shout out to ya Boi Dolossus

Terms : Hide Images
1311668985Lincoln Douglass DebatesEffort to solve crisis of Slavery had failed -=Debates fodcust on JUST SLAVERY + Future of Union Community swarmed to see these debates. Douglass: Vote against Slaver yin Kansas + Alienated South Democrats --Direct conflict w/ JAmes NBuchanan. Spoke for Union. Say Demos= Nat'l party and the Repubs were sectional. Appealed to Racism. SAID LINCOLN SUPPORT EQUALITY Lincoln: Entered as a Whig, radicalized bc of slavery. committed to freedom/ no slavery. *No believe in Equality* --Tried to be MODERATE --True Winners: spectators who saw politicians discus the issue.0
1311668986US Expansion + GrowthTriple in size. Population Quadruple. States Doubled. MAjority live West of Appalachian. Percapita Income 2X's --Cotton no longer DOMINANT USA= strong manufacture/agricultural exporter. --south's not as politically important since Cotton= not dominant --Expansion of Slavery wasn't urgent1
1312603350Politics Culture, National IdentityUSA= Superior bc of DEMOCRACY --We assume EUrope had Democratic Revolutions b/c we= Role Models! Democratic pride= lead to American Rennaissance? --National audience for scholars/wirters/newspapers --New Literature: Hawthone's Short Story. Some social critics, Moby Dick *Uncle tom's cabin*: combine 1st hand accounts by North Abilitionists/slaves. *2 million copies in 10 years.2
1312603351Congressional DebateCan't ignore Proviso anymore --Parties were created to override sectionalism! --Bc of Election 1848, Sectionalism erupted. Divided Relgious groups. **Final Action of Clay + Calhoun + Webster. Taylor died, was going to be like Jackson + Demand South comply to the North! --Millard Fillmore= weak president. **Calhoun= South must secede to protect state rights. Congress no right prohibit Slavery! Territory= common property of all states. Protect property! *Leaglly Correct definition of slaves* Southern Dogma **Webster: Secession= bad. PreserveUnion. Beg Demos compromise to keep south. **Secession revealed threat of "slave power" **Proslavery ppl: Keep as many slave states as free states. Antislavery ppl thought this was them trying to control Nat'l Politic3
1313030628Southern Dogma?ANYTHING LESS THAN FULL ACCESS OF THE TERRITORIES + OUR RIGHTS= UNCONSTITUTIONAL4
1313030629Slave Power?Rich white tht dominate political/social of South Conspire control fed gov't. Danger to Free speech + Institutions --Showed monolithic response of South Most ppl thought this was BS, but as South strategies to Expand slavery, veto power of Presidential nominees, and justification of "self defense", More ppl belive this true.5
1313030630Perspectives of Both SidesBoth want expansion: South want Slavery expand (Manifest Dest.) --Both want "Liberties". --Free Soilers= Personal Liberties, while South= Liberty to keep their way of life! Both Infringed eachother's Rights: South said North help slaves escape, spammed South w/ unwanted literature, abuse right to petition in congress, incite slave revolution North said: South Censor mails, Gag rule suppress free speech, Moral Wrong of enslave humans Stereotypes: North say: South were backwater, dominate by small slave owning aristocrat, deprive poor whites of honest work.. Suppress intelligence for entire country. We northerners offer economic opportunity to common man Southerners: say Slaves bring nat'l growth, North benefitted from slavery, Give white man independence, Our institution= good for slaves, Wage slavery in North.6
1313030631Compromise of 18501. California= free state, Put former Mexican Possessions decide by pop. Sov. 2. Utah: not accepted as a state until 1896 bc Mormon Polygamy (Give More Freestates, vs Slave states) 3. Texas: Cede land to NEw MEx. Gov't assume $10milly debts of TX 4. Slave Trade end in DC, NOT SLAVERY. 5.Stronger Fugitive Slave law for all states. --Still divides within Parties, Antislave Whigs fight vs. Proslave Whigs. **Sout Whigs + North Democrats were forces of MODERATION **Slavery avoided, not settled7
1313030632Fugitive Slave ActNorth urge Slave scape. Help + Support them. --Underground RR helped a little. --Most Slaves got help from North Freedmen Northerners HATE Slave Catchers. Slaves no allow to defend themselves+ Even Freedmen reshipped into Slavery --12 Years a Slave. Made 9States pass PERSONAL LIBERTY LAWS --No cooperate w/ recapture. **South Angry, already suspect North not cooperating. Infringe their rights --This increase power of owners. **Slave Given chance go to Hearing before Fed. commissioner. Not allowed to testify on own behalf. --Fines on ppl who help fugitives. --Many went to Canada, They not willing to send Slaves back to slavery tho. **This ACT made SLAVERY a REALITY for North8
1313030633Shadrach Minkins + Anthony BurnsBoston: Shadrach Minkins recaptured. Some freedmen stormed courtroom, send him to Ganada --Community support this, Ppl thought this was "mob rule" **Most Famous: Antrhony Burns. Failed to rescue him. President Pierce send: Marines + Calvary, Ship, to send him back to Slavery --Bostonian raise money buy his freedom back, but Pres blocked the purchase. --Buildings shrowded w/ Black drapes. USA flags upsided Down. Funeral Bells tolled. **This Radicalized Many North.9
1313030634Election of 1852:1st Sign that Nat'l Party system was weakening= difficult getting nominees. -Whigs: Winfield Scott over Fillmore --South Whigs feel alienated, Loyalty strained --WHIGS NEVER FIELDed PREZ. CANDIDATE AGAIN Demos: Large variety, Cass + Stephen Douglass, JAmes Buchanan --Chose Frank Pierce. : Want faithful execution of Comp. of 1850. Demos tht vote for Free Soil voted for Pierce + Irish/German voters. --Showed strength of Democraic Machines. (corruption + vote buying) **Pierce easily won10
1313030635Young America MovementLarge portion ppl support Pierce. Group of NY politicians desire to Conquer Cuba/Central America --Expansionists want move Southward. Several private *Filibusters* invade Carribbean/ Central American Countries to extend Slave territory --Will Walker 3 invasions to Nicaragua. Pierce: WANT CUBA. Not directly involved in Filibusters. -->Slave revolt in Cuba made Cuban slave owners want to be annexed to USA to continue slavery. **Ostend Manifesto: Mixture of threats**, South of USA + Cubans= 1 people. We will Physically wrest Cuba from Spain if need be. **Manifesto= supposed to be secret, leaked. Pierce had to repudiate it. *send Matt Perry to Japan. Treaty set up commerce btw us. --Pierce + Proslavery lost orth Goodwill tht Pierce had.11
1313030636Crisis of Nat'l Party systemStephen Douglass introduce Kansas-Neb. Act: --open up Indian Territory to US settlers under pop. Sov. --Gave possibiilty of Slavery out here! Try to satisfy EXPANSIoN + PRESIDENTIAL GOALS.12
1313030637Kansas Nebraska ActFurther construct RR across Great American Desert to CA. RR end @ chicago. Had to organize land as territories. Needed South support, had to open territories to slavery --Want Pop. Sovereignty dtmn Slavery in New Territory. VAGUE to appeal to both sides -- POSSIBILITY of slavery in new territory. **Repealed MO Compromise**: since it stopped slavery at 36'30 latitude. --North Whigs reject this absolutely. HUGE SPLIT, left them unable to pick Prez. Candidate. Cotton Whigs (economic ties w/ south, bankers, manufactureres, wealthy merchants) Hurt Democrats: North Demos lost 2/3 of seats in Congress. --*Biggest Miscalculation in Politics Ever*: +300 protests, Douglass rejected by North every time.13
1313107818Indians in the NEbraska Territory:Treaties made. Some agreed to relocate to small reserves. Some sell land to whites Some keep part of West Kansas...until gold found there. --Pro/Antislavery ppl poured into Kansas to Settle14
1313107819Bleeding KansasMISSOURIANS= 1st ppl get land in Kansas. Create proslavery placed (Kickapoo). Swamp KS elections w/ MO votes!. 6k Votes...when only 3k legal voters! --Cast by "Border Ruffians" frontiersmen. ***1st group of NEw Englanders establish LAWRENCE! Free Soil.l --Many Migrants= religious reformers. **BattleGround: Freesoilers got Rifles from East supporters, Ruffians Need reinforcements from 'Bama. Open warfare. Proslavery burnt/loot Lawrence. John Brown raid settlers, kill ppl. --Had armed group, roamed countryside burning + kiling. --Bands of Marauders never arrested/trialed/ stopped. --15
1313107820Politics of Nativism +American PartyMore violence in Nation's cities. --New Orleans had Vigilantes take over gov't. Fought @ Jackson Squre. vs. Catholics + Immigrants **Chicago Riots: Close saloons on Sunday, Germans/irish protest. NAt'l Guard involved *Lager Beer Riots*. led 2 MArtial law in Chicago **VIOLENCE CAUSED BY BREAKDOWN OF PARTY SYSTEM**: Whigs broke up + left political vaccum, Burst of Nativism/Antiimmirant feeling. **AMerican Party* formed to give expression to Nativism. since Democrats got many Catholic Foreign born voters! --When secret members questioned abt their beliefs, they say "I Know Nothing" dubbed the "Know Nothings" --Began to win elections in North + Control MAss. Legislature Polled well in South. It split into Pro/Anti slavery wings --Ppl began to vote for Republican Party16
1313107821Why did Democrats get support from Immigrants?Whigs hostile to Irish in Boston Whigs: no like Immigrants b/c they poor --Temperance movement, Not liked by Immigrants --Catholic sfear anti catholicism --Whigs hold immigrants responsible for increase in crime/cost of relief for poor --Nativism attract former whigs, young men of white collar/blue collar jobs17
1313107822REpublican aParty1. MAny former North Whigs who anti slavery 2. Free Soil Party supporters who oppose expansion of slave, but tolerate it in South 3. North Reformers concern abt. Temperance/Catholicism 4. Economic CORE of old Whigs.: Merchants/industriliasts who want strong nat'l gov't provide economic growth --Want protective tariff, transportation improvemens, Cheap land for West farms --.18
1313384473Election of 1856Demos: loosely held together --President Pierce vs. Stephen A Douglass: both support Kansas Nebr. Act. South support them, North didn't. Hard to Satisfy ANyONE. *Compromise Candidate= James Buchanan (North Man w/ South Ideas). Was in Britain, didn't have to pick a side to KansasNeb.Act. Election: Buch. vs. John Fremont vs. Mill Fillmore (American party) --2 Separate contests: North= Buch vs Fremont, South= Buch vs. Mill Fill. **Fremont's name appeared in only 4 South states. **Buchanan won bc he was the only NATIONAL CANDIDATE --Repubs= very close tho, "victorious defeat"!!! --They beat out American Party, but they weren't national. **Showed that voters like national candidates! Not sectional. **Slavery= worse than immigrants, Nativism subsided19
1313384474Dred Scott1. Roger Taney insist MO comp. Unconstitutional. Fed Gov't no right to interfere w/ free movement of property throughout territories. ...but Since Scott was black.. (not a citizen) 2. Buchanan pressured Northern Justice to agree w/ Taney... 3. Showed Sectional Decision. Southern opinion abt. slavery is Supreme law of the land! 4.North: question power of Supreme Court to establish law of the land 5. All NY people lost support for Supreme Court. 6. Fred Douglass urge blacks emigrate to...Haiti 7. This Swept away free-soil foundation of the Republicans. --Buchanan suspended the decision until he was inaugurated so that he could have influence on the decision as president20
1313384475Lecompton ConstitutionProslavery votes dominant bc border Ruffians illegal votes 1. Free soilers protest constt. + form their own gov't. --Now Pro slavery territory gov't + Free soil Gov't. --Proslavery majority write const. to Congress for Admission of Kansas as State. 2. Buchanan endorse Const. No want lose support of South Demos. Admit KS 16th slave state. HUGE FCKIN MISTAKE --**STEPHEN DOUGLASS OPPOSED const.** b/c it violate popular sovereignty --It hat to be voted by a legit election. He went against Buchanan (prez of his own party) + Then Almost all KS ppl reject the Const.! 3. Defeat of Const. led to ambushes/killinggs, mass shooting --Violence in Congress, 30 man free for all. --Buch lost all support of South Dmos.21
1313384476Panic of 18571. Failure of Ohio Investment House: Telegraphs to Wall street, etc. Mass paranoia/hysteria. Led to Busines failures/slowdowns. Threw 1000's our of work 2. Why? sharp temporary downturn of Agriculture exports to Britain --Affect South Cotton Less than Northern Goods --South said that this is proof tht their economic system= better than North's22
1313384477John Brown's raid1. He slaughtered proslavery men in KS. Now want Raid South + Start Slave uprising --Believe Slaves= just need 1 spark to get something going --No escape plan, didn't notify slaves for the uprising. 2. Brown captured + convict of treason/murder/insurrection/ maybe insane --Hanged. In North, communities had Mourning not seen since GEORGE WASHINGTON's DEATH!!--Church bells toll, buildings drape in blac, prayers, eulogies, 3. South suspect North ppl provke revolts --Brown had financial support of "secret six" of the north --Shocked by north Rxn to Brown's Death 4. Contary to popular belief, Repubs did not support Brown.23
1313502451Election of 18601. Split Demos into N+S wings= official. --Met @ Charleston (Center of secessionist agitation). Douglass not enough support for nomination --South want him support federal slave code to protect slavery --This was against his views, believe in pop.sov. + want North support too. --South not willing to compromise.South wing had own convention: Nominate John Breckinridge. 2. This split= Repub. Victory = Inevitable. Some South Wing Demos join Constitutional Union Party + nominate John Bell 3. Republicans: plan to carry all states Fremont got _ others. --Seward vs. Lincoln. Will= best known figure, had enemies thought he was too radical. --Lincoln= new, impressive, MODERATE. + Going to Carry ILlinois 4. VERY CLEAR CHOICES: Breckin ridge (extension slave to territories), LIncoln (EXCLUSION), , Bell (vague compromise) 5. Republicans: Homestead Act, Transcont RR, Higher Tariff. Denounc John Brown as Radical. Says they do not support EQUALITY. 6. Douglas= ONLY Candidate who spoke Urgently + about threat of secession. He campaigned personally in North/South. Most NATIONAL candidate -- Lincoln had many ppl speak for him, Repubs no campaign South, Breck no campaign North. 7. Lincoln's name not on ballots in the South! Secionalism. 2 regional contests: Breck vs. Bell in South, Linc vs. Doug in nNorth24
1313502452Rumors in the Deep SouthRumors of Slave revolts--> Cause vigilance committees spring uup 1. south Carolina= Secession dominated politics. -- Passion + excitement made South go towards extremeism. --Drought Made ppl want to secede!25
1313502453South Leaves the Union1. Country finally overthrown domination of slave owners w/ Lincoln --South shocked + humiliated. Permanent minority in politics now. --Lincoln threatened their way of life. Secession necessary 2. Gov'nors of SC, 'Bama, Miss, all commit states to secede if Linc. Elected. --Vigilance committees + Volunteer militia companies --Cooperationists= ppl who oppose secession, intimidated to silence 3. South Carolina: Unaimously secede from union. 6 other states (Miss, Fl, Ala, GA, LA, TX) . --Rapid secession= good strategy, do it before cooperationists organize! 4. Yeoman + city workers want compromise w/ North --But we must preserve WHITE PRIVILEGE26
1313502454North's Political Options1. Brekinridge Idea: No Compromise on Extension of Slave. Appear FIRM + Moderate. Discrouage states from secede, give pro-Union time to organize 2. Horace Greel: let 7 states go in peace. But north wasn't going to let this happen. Too much pride 3. Force go to war + Force states back into the union --No give up fed power over military forts/customs posts in the South (which the south Needed to become independent) 4. Abe's Idea: Just wait it out, see what happens, don't make us aggressors. Wanted to see if South could succeed , didn't want to be the "bad guys"27
1314127865Establishment of the Confederacy1. Delegates of 7 seceding state met in Montgomery --Wrote constitution identical to the Union's, strongly support state's rights + made abolition impossible 2. Defense of slavery: Commitment to individualism + decentraliztaion. Right of slaveowner over his slaves, freedom. 3. Needed strong fed gov't to have strong military...Didn't have one 4. Mont. Convention- chose Jeff. Davis + Alex Stephens. BOTH MODERATES --Stephens: urged secessionists not be taken quickly 5. Theme: Secession= natural/ not a big deal. Just let the south do their own thing. 6. @ Lincoln's Inaguration: He refuse issue public statements before presidency so he didnt MAKE SITUATION WORSE. --Say nothing controversial. USA no like, used to military leaders, not lawyers... Want instant leadership. *Nonbelligerent Moderation* We are their friends!28

How to Name Compounds Flashcards

Someone who doesn't know chemistry might think that compounds should already have names, but you know differently. The following steps take you through the process of building a chemical name, using compound XAYB as an example:

Terms : Hide Images
469939911Someone who doesn't know chemistry might think that compounds should already have names, but you know differently. The following steps take you through the process of building a chemical name, using compound XaYb as an example:Example: XaYb0
469930456Is X hydrogen?If so, the compound is probably an acid and may use a common name. If X isn't hydrogen, proceed to Step 2.1
469930457Is X a nonmetal or a metal?If X is a nonmetal, then the compound is molecular. For molecular compounds, use numeric prefixes before each element's name to specify the number of each element. If there's only one atom of element X, no prefix is required before the name of X. Use the suffix -ide after the element name for Y. If X is a metal, then the compound is ionic; proceed to Step 3.2
469930458Is X a metal that has variable charge?If X has a variable charge (often, these are group B metals), you must specify its charge within the compound by using a Roman numeral within parentheses between the element names for X and Y. For example, use (II) for Fe2+ and (III) for Fe3+. Proceed to Step 4.3
469930459Is Y a polyatomic ion?If Y is a polyatomic ion, use the appropriate name for that ion. Usually, polyatomic anions have an ending of -ate or -ite (corresponding to related ions that contain more or less oxygen, respectively). Another common ending for polyatomic ions is -ide, as in hydroxide (OH-) and cyanide (CN-). If Y is not a polyatomic ion, use the suffix -ide after the name of Y.4
4699399121Mono5
4699399132Di6
4699399143Tri7
4699399154Tetra8
4699399165Penta9
4699399176Hexa10
4699399187Hepta11
4699399198Octa12
4699399209Nona13
46993992110Deca14
469939922Hypo___ate2 less than ate15
469939923ite1 less than ate16
469939924ateAnion and Oxygen17
469939925Per___ate1more than ate18
469939926ideAnion with 2 elements19

Pages

Subscribe to CourseNotes RSS

Need Help?

We hope your visit has been a productive one. If you're having any problems, or would like to give some feedback, we'd love to hear from you.

For general help, questions, and suggestions, try our dedicated support forums.

If you need to contact the Course-Notes.Org web experience team, please use our contact form.

Need Notes?

While we strive to provide the most comprehensive notes for as many high school textbooks as possible, there are certainly going to be some that we miss. Drop us a note and let us know which textbooks you need. Be sure to include which edition of the textbook you are using! If we see enough demand, we'll do whatever we can to get those notes up on the site for you!