3606748188 | Viruses | Can exist intracellularly OR extracellularly. | | 0 |
3606748189 | Inactive | When viruses are extracellular, they are this. | | 1 |
3606748190 | Intracellular | When viruses exist primarily as replicating nucleic acids | | 2 |
3606748191 | Virions | Complete virus particle | | 3 |
3606748192 | Bacteriophages | Viruses that infect bacteria | | 4 |
3606748193 | Nucleocapsid | Simplest virion; nucleic acid + capsid | | 5 |
3606748194 | Capsid | Coating that protects the genome | | 6 |
3606748195 | Helical capsids | Hollow tubes with protein walls (ex. TMV) | | 7 |
3606748196 | Icosahedral capids | Regular polyhedron with 20 equilateral triangular faces + 12 vertices | | 8 |
3606748197 | Capsomers | Ring-shaped, morphological unit of which Icosahedral capsids are constructed | | 9 |
3606748198 | Pentamers | Have 5 protomer | | 10 |
3606748199 | Hexamers | Have 6 protomer | | 11 |
3606748200 | Protomers | Capsid proteins | | 12 |
3606748201 | Envelope | Outer membranous layer | | 13 |
3606748202 | Peptomers | Scientific name for "spikes". Can be used for attachment to host cell. | | 14 |
3606748203 | Viral genomes | dsDNA, ssDNA, dsRNA, ssRNA | | 15 |
3606748204 | 3,000-4,000 | How many nucleotides does a small virus have? | | 16 |
3606748205 | 1.2x10^6 | How many nucleotides does a large virus have? | | 17 |
3606748206 | Attachment | First step in viral multiplication | | 18 |
3606748207 | Entry into the host | Second step in viral multiplication | | 19 |
3606748208 | Synthesis stage | Third step in viral multiplication | | 20 |
3606748209 | Assembly | Fourth step in viral multiplication | | 21 |
3606748210 | Virion release | Last step in viral multiplication | | 22 |
3606748211 | Virulent | Begin multiplying immediately upon entering the host, then release by lysis | | 23 |
3606748212 | Temperate | Have 2 options:
1. Enter host cell, multiply like virulent, and burst cell. (LYTIC)
2. Remain within host without destroying it. (LYSOGENIC) | | 24 |
3606748213 | Prophage | Form of the virus that remains within the host cell | | 25 |
3606748214 | Lysogenic conversion | When a temperate phage changes the phenotype of its host | | 26 |
3606748215 | Cytopathic effects | Degenerative changes/abnormalities in host cells and tissues that are distinct from lysis. | | 27 |
3606748216 | Tumor | Growth or lump of tissue resulting from neoplasia | | 28 |
3606748217 | Neoplasia | Unregulated, abnormal, new cell growth and reproduction | | 29 |
3606748218 | Anaplasia | Invasive growth that forms unorganized cell masses | | 30 |
3606748219 | Benign tumor | Tumors that remain in place | | 31 |
3606748220 | Malignant tumor | Tumors that spread throughout the body | | 32 |
3606748221 | Oncogenes | A gene that has the potential to cause cancer | | 33 |
3606748222 | Oncoviruses | Viruses associated with cancer | | 34 |
3606748223 | Viroids | Infectious agents that only have RNA and cause plant diseases | | 35 |
3606748224 | Satellites | Have either DNA OR RNA but can encode their own gene products | | 36 |
3606748225 | Replisome | Carries out replication | | 37 |
3606748226 | Septation | Forming a cross wall between 2 daughter cells | | 38 |
3606748227 | Cytokinesis | Cytoplasm of a single cell is divided and forms 2 daughter cells | | 39 |
3606748228 | Extremophiles | Organisms that live in harsh conditions | | 40 |
3606748229 | Important environmental factors on microbial growth | - Solutes
- Water activity
- pH
- Temp
- O2 levels
- Pressure
- Radiation | | 41 |
3606748230 | Hypertonic | Too much water coming out. Cell shrivels up, higher concentration of solutes outside of cell | | 42 |
3606748231 | Hypotonic | Cell fills up with water and bursts. Lower osmotic pressure than surrounding medium | | 43 |
3606748232 | Isotonic | Balanced, normal state. Equal osmotic pressure between inside and outside of cell | | 44 |
3606748233 | Acidophiles | Grow @ optimum between pH of 0-5.5 | | 45 |
3606748234 | Neutrophiles | Grow @ optimum between pH of 5.5-8.0 | | 46 |
3606748235 | Alkalophiles | Grow @ optimum between pH of 8.0-11.5 | | 47 |
3606748236 | Cardinal temperatures | Minimum, maximum, and optimum growth temperatures. Optimum usually closer to maximum. | | 48 |
3606748237 | 0-75 degrees Celsius | Optima temperature for microorganisms | | 49 |
3606748238 | Psychrophiles | Cold environments; grow well at 0 degrees Celsius. Optimum temp is 15 degrees, max is 20. | | 50 |
3606748239 | Psychrotrophs | Grow @ 0 degrees. Max = 35. Major cause of food spoilage in refrigerator! | | 51 |
3606748240 | Mesophiles | Moderate environments. Optimum = 20-45 degrees Celsius. Max = 45. Min = 15-20. Most human pathogens!! | | 52 |
3606748241 | Thermophiles | Grow @ 45-85 degrees Celsius. Optima = 55-65. Mostly bacteria and archae that love HOT environments. | | 53 |
3606748242 | Hyperthermophiles | Optima = 85-115 degrees Celsius. Found in areas of the marine floor. | | 54 |
3606748243 | Oligotrophic environments | Environments in which nutrients are low. Starvation types of environments. | | 55 |
3606748244 | Culture medium | Solid or liquid; used to grow, transport & store mircoorganisms. | | 56 |
3606748245 | Defined (synthetic) media | All chemical components are known in this media | | 57 |
3606748246 | Complex media | Has some ingredients of unknown chemical composition | | 58 |
3606748247 | Agar | Solidifying agent | | 59 |
3606748248 | Supportive media | Media that sustains the growth of many microorganisms | | 60 |
3606748249 | Enriched media | Nutrients are added to support a media to encourage the growth of fastidia organisms | | 61 |
3606748250 | Selective media | Allows for the growth of particular microorganisms while inhibiting the growth of others | | 62 |
3606748251 | Differential media | Distinguish among different groups of microbes and even permits tentative identification of microorganisms based on their biological characteristics | | 63 |
3606748252 | Growth curve | Log # of viable cells vs. Time. Four "times" on the graph: Lag, Exponential (log), stationary phase, and death. | | 64 |
3606748253 | Log | First phase of growth curve. No immediate increase in cell #'s occurs. Cells are synthesizing new components. Eventually, cells begin to replicate, increase in weight, and divide. | | 65 |
3606748254 | Exponential (log) | Where microorganisms are growing and dividing at the maximum possible rate given their genetic potential and environmental conditions. Growth rate is constant. | | 66 |
3606748255 | Stationary phase | Nothing is happening, population growth ceases and the growth curve becomes horizontal | | 67 |
3606748256 | Senescence | Programmed cell death | | 68 |
3606748257 | Death | A fraction of the microbial population is genetically programmed to die after growth stops | | 69 |
3606748258 | Viable but not culturable | VBNC | | 70 |
3606748259 | Direct counts | Direct measurements of cell numbers. A counting chamber. | | 71 |
3606748260 | *Do practice examples* | Dillutions | | 72 |
3606748261 | Biocide | A chemical agent capable of destroying living organisms | | 73 |
3606748262 | Sterilization | Process by which all living spores, viruses, viroids, and prions are either destroyed or removed from a habitat. | | 74 |
3606748263 | Disinfection | Killing, inhibiting, or removal of microorganisms that may cause disease. Destruction of potential pathogens. | | 75 |
3606748264 | Disinfecants | Chemical agents used for disinfection. Used only on fomites (inanimate objects) | | 76 |
3606748265 | Sanitization | Microbial populations are reduced to levels considered "safe" by public health standards. Inanimate objects are cleaned as well as being partially disinfected. | | 77 |
3606748266 | Antiseptics | Chemical agents applied to tissue to prevent infection by killing or inhibiting pathogen growth. Not as toxic as disinfectants. | | 78 |
3606748267 | Antisepsis | Destruction of microorganisms on living tissues. | | 79 |
3606748268 | Chemotherapy | Use of chemical agents to kill or inhibit the growth of microorganisms within the host tissues. Includes antibiotics, antifungals, etc. | | 80 |
3606748269 | D-Value | Decimal reduction time. The time required to kill 90% of microorganisms or spores in a sample under specified conditions. | | 81 |
3606748270 | Filtration | Reduce the # of organisms in solutions of heat-sensitive material. | | 82 |
3606748271 | Kinds of filters | Depth, membrane, and HEPA (high efficiency particulate air filter) | | 83 |
3606748272 | Heat control methods | Moist heat destroys viruses, bacteria and fungi. Kills by degrading nucleic acids and denatures proteins/enzymes. | | 84 |
3606748273 | Autoclave | Heat sterilization contraption. 121 degrees Celsius at 15 PSI | | 85 |
3606748274 | UV Radiation | Good. Doesn't penetrate glass, dirt films, water and other substances. Used only in specific situations. | | 86 |
3606748275 | Ionizing Radiation | Great! Penetrates deep into objects. | | 87 |
3606748276 | Phenolics | First widely used antiseptics and disinfectants. Includes: cresols, xylenols, orthophenols and lysol. | | 88 |
3606748277 | Alcohols | Most widely used antiseptics and disinfectants. Affects bacterial and fungicidal, NOT sporocidal. Includes: ethanol and isopropyl. | | 89 |
3606748278 | Halogens | Any of the 5 elements in group 7A of the periodic table. Iodine and chlorine are most important (antiseptics) | | 90 |
3606748279 | Iodophor | Water soluble, stable and non-staining, used to clean pre-op skin. | | 91 |
3606748280 | Chlorine | Used for disinfecting municipal water supplies, dairy and food industry. | | 92 |
3606748281 | Heavy metals | No longer used for cleaning because of how toxic they are. Mercury, silver, arsenic, and zinc. | | 93 |
3606748282 | Quaternary Ammonium Compounds | Detergents with broad spectrum antimicrobial properties. Disinfectants. | | 94 |
3606748283 | Aldehydes | Formaldehyde and glutaraldehyde. Sporicidal "fixatives" | | 95 |
3606748284 | Ethylene Oxide | Sterilizing gas | | 96 |
3606748285 | Antimicrobial agent effectiveness | Influenced by: Population size, population composition, concentration or intensity of antimicrobial agent, contact time, and temperatute. | | 97 |
3606748286 | Larger | For antimicrobial agent effectiveness, ______ population requires longer time. | | 98 |
3606748287 | Composition | Effectiveness of antimicrobial agent varies with the type of organism or population _________. Remember: spores. | | 99 |
3606748288 | Concentrated | Usually the more ___________, the more rapidly organisms are killed. Exceptions include 70% ethanol water more effective than 90% ethanol. | | 100 |
3606748289 | Longer | The ________ an organism is exposed, the more microbes that are killed. (Contact time) | | 101 |
3606748290 | Increase | An __________ in temperature usually enhances the antimicrobial. | | 102 |