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AP- Biology - Enzymes Flashcards

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7847647776chemical reactionA process that changes one set of chemicals into another set of chemicals.0
7847647777reactantscompounds that enter into a chemical reaction1
7847647778productcompounds produced by a chemical reaction.2
7847647779enzymebiological catalysts usually globular that speed up the rate of chemical reactions3
7847647781Competitive inhibitionsubstance that resembles the normal substrate competes with the substrate for the active site4
7847647782Noncompetitive inhibitora chemical that binds to an enzyme but not in the active site. This chemical will change the shape of the enzyme (reversible)5
7847647783substratethe substance an enzyme catalyzes, changes.6
7847647784active sitethe location on the enzyme where the substrate binds and goes through a chemical reaction.7
7847647785exothermic reactiona chemical reaction where energy is given off, so that the products have less energy than the reactants.8
7847647786endothermic reactiona chemical reaction where energy is taken in, so that the products have more energy than the reactants.9
7847647787activation energyEₐ is the abbreviation used for the energy required to start a reaction.10
7847647789Catalasean enzyme found in most aerobic organisms that breaks down H2O2 to water and oxygen11
7847647791lipaseEnzyme that can break the bonds of lipids to form the monomer, fatty acids.12
7847647792proteaseEnzyme that can break the polypeptide bonds of proteins to form the monomer, amino acids.13
7847647793nucleaseEnzyme that can break the bonds of nucleic acids to form monomer, nucleotides14
7847647795lock and key hypothesisThe substrate fits the active site of the enzyme like a key fits in a lock. There is no change to the shape of the enzyme or substrate.15
7847647796induced fit hypothesisThe active site of the enzyme is flexible and conforms to fit the substrate like a glove fits on a hand.16
7847647797DenatureCharacteristic of proteins; a change in shape that stops the protein from functioning.17
7847647798Allosteric__________ regulation of enzyme occurs when a molecule binds to an enzyme changing the protein's shape18
7847647799Catalyst______ an agent that speeds up a chemical reaction without itself being permanently altered19
7847647800GAn exergonic reaction releases free energy. The abbreviation for free energy is: Named after the American Scientist Josiah Gibbs20
7847647803Substrate orientationWhen Enzyme bring together specific atoms into a correct position that are otherwise rotating and tumbling so that bonds can form21
7847647811Shapein Biology the prefix allo means "different" and stereos means "__________"22
7847647812HydrogenThe specificity and activity of an enzyme depends on it 3D structure and this in turn depends on ______________ bonds23
7847647813hydrophobicChanges in H+ ions (acidity) concentration can alter how _____________ some regions of protein are.24
7847647814pHAfter looking at the graph the enzyme activity of the the three different enzymes is being regulated by what variable25
7847647815TemperatureAfter looking at the shape of graph the enzyme activity of this enzymes is being regulated by what variable:26
7847647816Substrate ConcentrationAfter looking at the shape of graph the enzyme activity of this enzymes is being regulated by what variable:27

AP Biology - Biotechnology Flashcards

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6474997519Genetic EngineeringProcess of manipulating genes & genomes0
6474998511BiotechnologyProcess of manipulating organisms or their components for the purpose of making useful products1
6475000660Recombinant DNADNA that has been artificially made using DNA from different sources (eg. human gene inserted into E. coli)2
6475004386Gene CloningProcess by which scientists can produce multiple copies of specific segments of DNA that they can work with in the lab3
6475007849Restriction EnzymeUsed to cut strands of DNA at specific locations (restriction sites)4
6475009563Restriction FragmentsHave at least 1 sticky end (single stranded end)5
6475011385DNA LigaseJoins DNA fragments6
6475012270Cloning VectorCarries the DNA sequence to be cloned (eg. bacterial plasmid)7
6475016013TransformationBacteria takes up plasmid (w/ gene of interest)8
6475017786PCR (Polymerase Chain Reaction)Amplify (copy) piece of DNA w/out use of cells9
6475018686Gel ElectrophoresisUsed to separate DNA molecules on basis of size & change using an electrical current (DNA --> + pole)10
6475023042NegativeThe charge of DNA11
6475025557DNA FingerprintUnique "picture" of a person's cut up DNA fragments that are separated by size12
6475028398DNA Microarray AssaysStudy many genes at same time13
6475032590Nuclear TranslationNucleus of egg is removed and replaced nucleus of the body cell14
6475036091Stem CellsCan reproduce itself indefinitely and produce other specialized cells15
6475039105ZygoteStem cell that is totipotent (any type of cell)16
6475042807Embryonic Stem CellStem cell that is pluripotent (many types of cells)17
6475043941Adult Stem CellStem cell that is multipotent (a few cell types) or induced pluripotent (iPS; forced to be pluripotent)18
6475051605RFLPs (Restriction Fragment Length Polymorphism)Cuts DNA with restriction enzymes -each person has a different # of DNA fragments created Analyze DNA samples on a gel for disease diagnosed *outdated method, required a lot of blood19
6475057499STR Analysis (Short Tandem Repeats)Non-coding DNA has regions with sequences (2-5 base length) that are repeated -each person has different # of repeats at different locations (loci) *current method of DNA fingerprinting (only needs 20 cells for analysis)20
6475065159GM (Genetically Modified) OrganismOrganisms altered through recombination DNA technology Insert foreign DNA into genome or combine DNA from different genomes21
6514472588PlasmidA small, circular, double-stranded DNA molecule that carries accessory genes separate from those of a bacterial chromosome. Also found in some eukaryotes, such as yeasts22
6514494765Sticky EndWhat a DNA fragment has after being cut by a restriction enzyme23
6514513990EpigeneticsChanges in organisms caused by modification of gene expression rather than the genetic code itself24
6514561854Short Tandem Repeats (STR)Repeated sequences of noncoding DNA that occur at different lengths and locations depending on the individual Current method of DNA fingerprinting, only needs 20 cells for analysis25

Biology AP : Enzymes Flashcards

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4884929131MetabolismThis is the word to describe all of the chemical reactions in an organism.0
4884933748Anabolic ReactionThis is a type of reaction that needs energy. ( ex: endothermic, endergonic, and dehydration )1
4884944897Catabolic ReactionThis is a type of reaction that gives off energy. ( ex. hydrolysis, exothermic, and exergonic )2
4884998637+10 to -10What is the scale for metabolism?3
4885001935ThyroidWhich organ deals with metabolism?4
4885004807EnzymeThis speeds up chemical reactions.5
4885019015ShapeThe enzyme needs to be specific to a single reaction. What makes enzymes specific? Note that reactants must fit into the enzyme.6
4885046431The enzyme lowers the activation energy and increases the collisions between molecules.How do enzymes speed reactions up?7
4885081848The enzyme gets it's shape by the way it folds.How does the enzyme get it's shape?8
4885151098Kinetic EnergyThis is the energy of motion. ( Energy at work )9
4885153358Potential EnergyThis is stored energy. ( ex: energy in a CH bond )10
48851625631. Energy cannot be created or destroyed. 2. When you change the form of energy, you release heat. ( ex: potential to kinetic energy releases heat or entropy )Name the Two Laws of Thermodynamics.11
4885174038HeatThis is random energy. It is hard to grab or recycle. Over time, the earth becomes more random because this is random.12
4885259224It has a phosphate group, a ribose sugar, and an adenine.How does ATP resemble a nucleotide?13
4885307053The phosphates have high energy bonds because the negatives on the oxygen repel each other.Why is it so easy to break the bond between phosphates in an ATP?14
4885361976MitochondriaWhat organelle reassembles the ADP, phosphate, and energy into ATP?15
4885371686It uses energy from chopped CH bonds in sugars ( carbohydrates ).How does the mitochondria reassemble the ATP?16
4885382813You couple the endergonic reaction to hydrolysis of ATP.To do an endergonic reaction, you need energy. Where do you get the energy?17
4885417513The change in G ( free energy )What is the product minus the reactant?18
4885491766Activation EnergyThe minimum amount of energy required to start a chemical reaction.19
4885501137Free EnergyThis is energy that is available to do work.20
4885523826Free Energy Concentration EquilibriumWhat three things are not affected by the enzyme at all?21
4885538261Active SiteThis is the part of the enzyme that the reactant or substrate binds to.22
4885555639Induced FitWhen the substrate binds to the enzyme, the bind will twist a little to grip each other. What is this called?23
4885624771The reaction reverses and goes back and forth.When the amount of products and reactants become equal, the reaction does what?24
4885631311You can measure the amount of product being made or the reactant being used.How do we see how quickly the reaction is going?25
48856455781. temperature 2. pH 3. amount of substrateWhat are the three things that affect the rate of reaction?26
4885688148Transition StateThis is where all of the substrate is bound to an enzyme.27
4885729953Competitive InhibitorA substance that reduces the activity of an enzyme by entering the active site in place of the substrate whose structure it mimics.28
4885734763Noncompetitive InhibitorA substance that reduces the activity of an enzyme by binding to a location remote from the active site, changing its conformation so that it no longer binds to the substrate.29
4885747903Allosteric SiteA site on an enzyme other than the active site, to which a specific substance binds, thereby changing the shape and activity of the enzyme.30
4885767531ActivatorA protein that binds to DNA and stimulates transcription of a specific gene.31
4885770032InhibitorA substance that slows down or stops a chemical reaction.32
4885784827Metabolic PathwayA series of chemical reactions that either builds a complex molecule (anabolic pathway) or breaks down a complex molecule into simpler compounds (catabolic pathway).33

Cells - AP Biology Flashcards

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6411346614cellsthese are life's basic units of structure and function; all living things are composed of these These are the smallest units of living material that can carry out all the activities necessary for life.0
6411354397light microscopean instrument used to study stained or living cells (can see bacteria)1
6411357987electron microscopean instrument used to study detailed structures of a cell capable of resolving structures as small as a few nanometers in length2
6411364866eukaryotic (cells)cells that contain a membrane-bound structure called a nucleus and cytoplasm, filled with tiny structures called organelles Ex.) fungi, protists, plant cells, animal cells3
6411368699prokaryotic (cells)smaller cells that lack both a nucleus and membrane-bound organelles; most of these have a cell wall composed of peptidoglycan, and may also have ribosomes as well as one of more flagella The genetic material in one of these cells is one continuous, circular DNA molecule that lies free in the cell in an area called the nucleoid. Ex.) bacteria4
6411394303nucleoidThis structure in a prokaryote contains one continuous, circular DNA molecule that lies free in the cell.5
6411399120peptidoglycanFor most prokaryotes, this substance composes the cell wall.6
6411401816flagellalong projections from prokaryotes used for motility (movement)7
6411417867plasma membranean outer envelope of a cell that is a complex, double-layered structure made up of phospholipids and proteins (the hydrophobic fatty acid tails face inward and the hydrophilic phosphate heads face outward) This is important because it regulates the movement of substances into and out of the cell. It is semipermeable, meaning that only certain substances, namely small hydrophobic molecules, pass through it unaided.8
6411437852peripheral proteinsproteins loosely bound to the plasma membrane (not embedded within) that are located on the inner or outer surface of the membrane9
6411449217integral proteinsproteins that are firmly bound to the plasma membrane that are amphipathic penetrate the hydrophobic core of the lipid bilayer, often completely spanning the membrane (as transmembrane proteins, meaning that they do not extend all the way through the membrane)10
6411460369amphipathicterm used to describe proteins whose hydrophilic regions extend out of the cell or into the cytoplasm while their hydrophobic regions interact with the tails of the membrane phospholipids11
6411472338transmembrane proteinsintegral proteins spanning the membrane that do not extend all the way through the plasma membrane12
6411482685fluid mosaic modela diagram of the arrangement of phospholipids and proteins13
6411509539adhesion proteinsplasma membrane proteins that form junctions between adjacent cells attach cells to neighboring cells or provide anchors for the internal filaments and tubules that give stability to the cell14
6411514710receptor proteinsplasma membrane proteins that serve as docking sites for proteins of the extracellular matrix or hormones accepts signals and initiates responses; sensitive to the presence of specific extracellular molecules called ligands15
6411523689transport proteinsplasma membrane proteins that form pumps that use ATP to actively transport solutes across the membrane helps a certain substance or class of closely related substances to cross the membrane16
6411533843channel proteinsplasma membrane proteins that form channels that selectively allow the passage of certain ions or molecules proteins that provide passageways through the membrane for certain hydrophilic (water-soluble) substances such as polar and charged molecules17
6411554465recognition and adhesion proteinsplasma membrane proteins, such as glycoproteins, that are exposed on the extracellular surface and play a role in cell regulation and adhesion18
6417273915carbohydrate side chainsthese are attached to the surface of some proteins on phospholipid bilayer19
6519838002cholesterolthese molecules are found in the phospholipid bilayer because they help to stabilize membrane fluidity in animal cells20
6519840427nucleusthe largest organelle that is the control center of the cell; contains chromosomes that contain heredity information it not only directs what goes on in the cell- it is also responsible for the cell's ability to reproduce21
6519849777chromosomeslarge structures in the nucleus of a cell that are organized DNA22
6519854570nucleolusthe most visible organelle within the nucleus where rRNA is made and where ribosomes are assembled23
6519865348ribosomesthe sites of protein synthesis; their job is to manufacture all the proteins required by the cell or secreted by the cell round structures composed of two subunits; the structure is composed of RNA and proteins; they can be either free floating in the cell or attached to the endoplasmic reticulum24
6519882035endoplasmic reticuluma continuous channel that extends into many regions of the cytoplasm; classified as two regions: rough and smooth25
6519890675rough ERthe region of the ER that is "studded" with ribosomes proteins generated here are trafficked to or across the plasma membrane26
6519895203smooth ERthe region of the ER that lacks ribosomes makes lipids, hormones, and steroids and breaks down toxic chemicals27
6519959166Golgi bodiesorganelles that look like stacks of flattened sacs that modify, process, and sort the products after the ribosomes on the rough ER have completed synthesizing proteins They're the packaging and distribution centers for materials destined to be sent out of the cell. They package the final products in little sacs called vesicles, and they are also involved in the production of lysosomes.28
6520025569mitochondriathe "powerhouses" of the cell that are responsible for converting the energy from organic molecules into useful energy for the cell (ATP) has a double membrane consisting of an inner portion and an outer portion; the inner membrane forms folds known as cristae, which is where most of the production of ATP is done Since they are the cell's powerhouses, you're likely to find more of them in cells that require a lot of energy. Muscle cells, for example, are rich in mitochondria.29
6520115097ATP (adenosine triphosphate)the energy molecule used by cells30
6520117406lysosomessmall, membrane-bound structures that carry digestive enzymes, which they use to break down old, worn-out organelles, debris, or large ingested particles They make up the cell's cleanup crew, helping to keep the cytoplasm clear of unwanted flotsam. They contain hydrolytic enzymes that only function at acidic pH, which is enclosed inside the structure's lumen.31
6520218552centriolessmall, paired, cylindrical structures that are found within microtubule organizing centers (MTOCs) and they are most active during cellular division When a cell is ready to divide, they produce microtubules, which pull the replicated chromosomes apart and move them to opposite ends of the cell.32
6520260391vacuole"empty cavity" in the cell that is a fluid-filled sac that stores water, food, wastes, salts, or pigments33
6520287779peroxisomesorganelles that detoxify various substances, producing hydrogen peroxide as a byproduct; they also contain enzymes that break down hydrogen peroxide into oxygen and water In animals, they are common in the liver and kidney cells.34
6520304933cytoskeletona network of fibers that determines the shape of a cell; these fibers include microtubules and microfilaments35
6520312107microtubulesmade up of the protein tubular, they participate in cellular division and movement; these small fibers are an integral part of three structures: centrioles, cilia, and flagella36
6520320767ciliahairlike projections that extend from the plasma membrane and are used for locomotion37
6520346627flagelluma long, whip-like filament that helps in cell motility38
6520354294Euglenaunicellular organism that moves using its flagella; involved in asexual reproduction and has chloroplasts to absorb sunlight39
6520364737Parameciuma single-celled freshwater animal that has a characteristic slipperlike shape and is covered with cilia; its cilia enable it to motor about in waterways and ponds40
6520374754microfilamentsimportant for movement like microtubules; these thin, rod-like structures are composed of the protein actin, are involved in cell mobility, and play a central role in muscle contraction41
6520442540cell walla structure found only in plant cells that is made of cellulose and is a rigid layer just outside of the plasma membrane that provides support for the cell It is found in plants, protists, fungi, and bacteria.42
6520454365chitina modified polysaccharide that makes up the cell wall in fungi43
6520492564chloroplastsorganelles in plant cells that are involved in photosynthesis; they contain chlorophyll, the light-capturing pigment that gives plants their characteristic green color44
6520499895cell sapa watery liquid in a plant cell's vacuole composed of dissolved sugars, mineral ions, and other solutes45
6520589713plant celltype of cell that... -has a cell wall -has chloroplasts -has a very large vacuole -does not have centrioles46
6520601368animal celltype of cell that... -does not have a cell wall -does not have chloroplasts -has small vacuoles -has centrioles47
6520655309waterThe lipid membrane has an open-door policy for substances that are made up of lipids. If a substance is hydrophilic, the lipid bilayer won't let it in. What is the one exception to the rule? Although ______ molecules are polar (and therefore not lipid-soluble), they can rapidly cross a lipid bilayer through aquaporins, which are integral membrane proteins that regulate the flow of ______.48
6520694814lipid_____-soluble substances can traverse the plasma membrane without much difficulty.49
6520697179simple diffusiondiffusion in which if there's a high concentration of a substance outside the cell and a low concentration inside the cell, the substance will move into the cell In other words, the substances moves down a concentration gradient.50
6520706836passive transportsimple diffusion is another name for this kind of transport because it does NOT require energy51
6520729060osmosisa special type of diffusion that involves the movement of water Ex. ) If a chamber containing water and a chamber containing a sucrose solution are connected by a semipermeable membrane that allows water but not sucrose to cross, diffusion of sucrose between the chambers cannot occur. In this case, _____ draws water into the sucrose chamber to reduce the sucrose concentration. This will reduce the total volume of the water chamber. Water will flow into the sucrose chamber until the concentration is the same across the membrane.52
6520762521isotonicterm referring to a substance having the same solute concentration as another solution53
6520747023hypertonicterm referring to substance having a higher concentration of solute than another solution54
6520750241hypotonicterm referring to substance having a lower concentration of solute than another solution55
6520877403tonicitythe ability of a surrounding solution to cause a cell to gain or lose water If the environment is isotonic to the cell, the solute concentration is the same inside and outside. A hypertonic solution has more total dissolved solutes than the cell, a hypotonic solution has less.56
6520906794water potentialthe measure of potential energy in water ad describes how water flows, from an area of high water potential to an area of low water potential it is affected by two factors: pressure potential and solute potential (formula to find SP is the picture)57
6520923216expandA red blood cell dropped into a hypotonic solution (such as distilled water) will _______, because water will move into the cell, to an area of lower water potential. Eventually, the red blood cell will pop.58
6520930737concentrationThe _____ of a solution can be calculated by dividing the number of moles of solute by the volume (in liters) of a solution. CiVi = CfVf59
6520951553solutesdissolved substances60
6520953479facilitated diffusiondiffusion in which channel proteins pick up a substance from one side of the membrane and carry it across to the other; does NOT require energy61
6520969779active transportmovement against the natural flow; transport that REQUIRES energy Suppose a substance wants to move in the opposite direction- from a region of lower concentration to a region of higher concentration. A transport protein can help usher the substance across the plasma membrane, but it's going to need energy to accomplish this.62
6521077630sodium potassium pumpthis special protein involved in active transport ushers out sodium ions and brings in potassium ions across the cell membrane These pumps depend on ATP to get ions across the would otherwise remain in regions of higher concentration. These proteins are usually found in neurons and skeletal muscle fibers.63
6521092328endocytosisthe process in which the cell uses a portion of the cell membrane to engulf a large substance The cell membrane forms a pocket, pinches in, and eventually forms either a vacuole or a vesicle.64
6521120119pinocytosistype of endocytosis in which the cell ingests liquids ("cell-drinking")65
6521124193phagocytosistype of endocytosis in which the cell takes in solids ("cell-eating")66
6521130469receptor mediated (endocytosis)type of endocytosis in which a particle (ligand) binds to one of the cell surface receptors that are covered in clathrin-coated pits and is brought into the cell by the invagination or "folding in" of the cell membrane; a vesicle then forms around the incoming ligand and carries it into the cell's interior67
6521149719bulk flowthe one-way movement of fluids brought about by pressure For instance, the movement of blood through a blood vessel or movement of fluids in xylem and phloem of plants are examples of this process.68
6521162617dialysisthe diffusion of solutes across a selectively permeable membrane For example, a cellophane bag is often used as an artificial membrane to separate small molecules from large molecules.69
6521169937exocytosisprocess in which a cell ejects waste products or specific secretion products, such as hormones, by the fusion of a vesicle with the plasma membrane70
6521179317intercellular junctionswhen cells come in close contact with each other, they develop these that involve their plasma membranes as well as other components These structures may allow neighboring cells to form strong connections with each other, prevent passage of materials, or establish rapid communication between adjacent cells.71
6521323380desmosomesthese hold adjacent animal cells tightly to each other, like a rivet; they consist of a pair of discs associated with the plasma membrane of adjacent cells, plus the intercellular protein filaments that cross the small space between them72
6521335249gap junctionsprotein complexes that form channels in membranes and allow communication between the cytoplasm of adjacent animal cells or the transfer of small molecules and ions73
6521351506tight junctionsmembranes of neighboring cells are actually fused forming continuous belts around cell to prevent leakage of extracellular fluid74
6521358199quorum sensingcell to cell chemical communication75
6521362791taxisthe movement of an organism in response to a stimulus, and can be positive (towards the stimulus) or negative (away from the stimulus)76
6521367067chemotaxisa cellular movement in response to chemicals Ex. ) Bacteria can control flagella rotation to direct their motion, thus avoiding repellents (such as poisons), or helping them find favorable locations with high concentrations of attractants (such as food). In our bodies, neutrophils use this to respond to an infection and are the first responders to inflammation.77
6521382328signal transductionprocess by which an external signal is transmitted to the inside of a cell 1. a signaling molecule binds to a specific receptor 2. activation of a signal transduction pathway 3. production of a cellular response78
6521392996G protein linked receptorthis receptor doesn't act as an enzyme but transducer its signal to enzymes in the cytoplasm using a second messenger One important second messenger is cyclic AMP. It is known as a "universal hunger signal" because it is the second messenger of the hormones epinephrine and glucagon, which cause energy mobilization. Second messengers are usually small molecules that can diffuse through the cell. They can be made and destroyed quickly and help the signal amplify throughout the cell.79
6521411774catalytic receptorreceptor that has an enzymatic active site on the cytoplasmic side of the membrane Enzyme activity is initiated by ligand binding at the extracellular surface. The insulin receptor is an example of an enzyme-linked receptor. After binding insulin, it initiates a complex signaling pathway that allows the cell to grow, synthesize lipids, and import glucose.80
6521423255ligand gated ion channelsthese channels in the plasma membrane open an ion channel upon binding a particular neurotransmitter An example is the channel on the surface of a skeletal muscle cell at the neuromuscular junction. This channel opens in response to acetylcholine, and a massive influx of sodium depolarizes the muscle cell and causes it to contract.81

AP Biology- Gene Regulation Flashcards

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6247440095operonconsist of a promoter, an operator, and a cluster of genes that control gene regulation in a common pathway, "on and off" switches0
6247440096repressible operonoperon that is normally on because the repressor is inactive1
6247440097inducible operonoperon that is normally off because the repressor is active2
6247440098structural genegenes to be expressed3
6247440099operatora segment of DNA in the promoter region that acts as an on or off "switch"4
6247440100repressora protein that binds to the operator to switch the operon off5
6247440101inducerinactivates the repressor (turns on the operon)6
6247440102regulatory genecodes for the production of the repressor protein and is found upstream of the operon7
6247440103corepressorworks with the repressor to turn off the operon8
6247440104catabolite activator protein (CAP)activator of transcription, binds to cAMP (cyclic adenosine monophosphate) when sugar is scarce which *increases the affinity of RNA polymerase and accelerates transcription of lactose*9
6247440105cellular differentiationthe formation of cells that are specialized in structure and function10
6247440106histone acetylationthe process of acetyl groups attaching to histone tails (opens up chromatin structure)11
6247440107DNA methylationadding a methyl group to cytosine bases during replication, associated with inactivation of genes and reduced transcription12
6247440108enhancergroups of distal control elements located upstream of downstream from a gene or within an intron13
6247440109activatora specific protein that binds to an enhancer to stimulate gene transcription14
6247440110transcription factorssome act as repressors by blocking the binding of activators or binding to an enhancer15
6247440111RNA interferencethe phenomenon of inhibition of gene expression by non-coding RNA molecules (a technique used to silence the expression of selected genes)16
6247440112RNA polymerasean enzyme that links ribonucleotides into a growing RNA chain during transcription17
6247440113miRNAa.k.a. microRNA, associates with one or more proteins in a complex that can degrade or prevent translation of an mRNA with a complementary sequence18
6247440114siRNAa.k.a. small interfering RNA, associates with one or more proteins in a complex that can degrade or prevent translation of an mRNA with a complementary sequence19
6247440115cytoplasmic determinantsa maternal substance that when placed into an egg influences the course of early development by regulating the expression of genes that affect the developmental fate of cells20
6247440116inductionthe process in which one group of embryonic cells influences the development of another, usually by causing changes in gene epression21
6247440117morphogenesisthe cellular and tissue based processes by which an animal body takes shape22
6247440118proto-oncogenea normal cellular gene that has the potential to become an oncogene23
6247440119ras genea gene that codes for Ras (a G protein that relays a growth signal from a growth factor receptor on the plasma membrane to a cascade of protein kinases) ultimately resulting in stimulation of the cell cycle24
6247440120tumor suppressor genea gene whose protein product inhibits cell division thereby preventing the uncontrolled cell growth that contributes to cancer25
6247440121p53 genea tumor suppressor gene that codes for a specific transcription factor that promotes the synthesis of proteins that inhibit the cell cycle26
6247440122negative feedbackinhibits expression27
6247440123positive feedbackactivates expression28
6247440124feedback inhibitionconcentration of substances present in cell either inhibit or activate expression29
6247440125cataboliclac operon30
6247440126anabolictrp operon31
6247440127heterochromatinharder transcription initiation32
6247440128epigeneticsthe field of science that studies heritable changes caused by the activation and deactivation of genes without any change in the underlying DNA sequence of the organism33
6247440129epigenomethe chemical compounds that modify or mark the DNA and change the way genes are expressed34
6247440130alternative DNA splicingproduces different mRNA molecules when different segments of the mRNA are chosen as introns or exons35
6247440131chromatin modificationpromotes the formation of heterochromatin in certain regions blocking transcription36
6247440132cell divisionthe production of large numbers of cells37
6247440133determinationthe condition in which a cell is irreversibly committed to its fate, even if it has not developed its final structure38
6247440134pattern formationthe development of a spatial organization of tissues and organs into their locations under the control of body plan genes39
6247440135positional informationmolecular cues provided by cytoplasmic determinants and inductive signals40
6247440136HOX genes (homeotic genes)primary genes responsible for head to toe formation41
6247440137oncogenescancer causing cells42
6247440138amplificationincreasing the number of copies of the gene43
6247440139tumor virusesviruses that insert their DNA into the host DNA near a proto-oncogene44
6247440140ectodermskin/nervous system45
6247440141mesodermmuscles46
6247440142endodermdigestive system47

AP Biology- Biochemistry Flashcards

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8038640719ionic bondbond resulting from a transfer of electrons causing an attraction between oppositely charged ions0
8038640720covalent bondA chemical bond that involves sharing a pair of electrons between atoms in a molecule1
8038640721nonpolara molecule in which all atoms have the same electronegativity and the electron distribution is equal, hydrophobic2
8038640722polarmolecule in which the positive and negative charges are separated and unequal, hydrophilic3
8038640723buffermixture that can react with an acid or a base to maintain the pH within a specific range4
8038640724isomerorganic compounds with the same molecular formula but different structure5
8038640725monosaccharideA single sugar molecule such as glucose or fructose, the simplest type of sugar; monomer of carbohydrates6
8038640726disaccharideA double sugar, consisting of two monosaccharides joined by dehydration synthesis.7
8038640727dehydration synthesisA chemical reaction in which two molecules are bonded together with the removal of a water molecule.8
8038640728hydrolysisBreaking down complex molecules (polymers) by the chemical addition of water.9
8038640729polysaccharideA polymer of many monosaccharides, formed by dehydration reactions.10
8038640730cellulosestructural polysaccharide that makes up plant cell walls11
8038640731starchStorage polysaccharide found in plant cells that consists entirely of glucose monomers12
8038640732glycerolThree-carbon compound with three hydroxyl groups; fatty acids are covalently bonded to it to make fats and oils.13
8038640733fatty acidhydrocarbon chain with a carboxyl group at one end; majority portion in lipids14
8038640734saturated fatA lipid made from fatty acids that have no double bonds between carbon atoms; solid at room temperature and found in animal fats, lards, and dairy products.15
8038640735unsaturated fatA lipid made from fatty acids that have at least one double bond between carbon atoms; liquid at room temperature and found in vegetable oils, nuts, and seeds.16
8038640736steroidlipid with a four fused ring structure; cholesterol and testosterone17
8038640737peptide bondbond creating amino acid chains or polymers18
8038640738primary structurelinear sequence of amino acids; peptide bonds19
8038640739secondary structureEither an alpha helix or beta pleated sheet formed with hydrogen bonds20
8038640740tertiary structure3D shape of single polypeptide due to interactions of the side chains using hydrogen bonding, ioning bonding and disulphide bridges21
8038640741quaternary structureThe shape resulting from the association of two or more polypeptide subunits.22
8038640742alpha helixsecondary structure form of a protein; human hair (keratin)23
8038640743beta pleated sheetsecondary structure form of a protein; spider webs and silk24
8038640744functional groupgroup of atoms within a molecule that interacts in predictable ways with other molecules25
8038640745cohesionattraction between molecules of the same substance; Water molecules sticking to each other.26
8038640746polymerlong molecule consisting of many similar or identical monomers linked together27
8038640747monomerbuilding block for polymers; only made by producers28
8038640748adhesionattraction between different kinds of molecules; Water molecules sticking to other surfaces.29
8038640749surface tensionmeasure of how difficult it is to stretch or break the surface of a liquid30
8038640750van der Waals interactionstertiary structure; weak attractions between molecules or parts of molecules that result from localized charge fluctuations31
8038640751disulfide bridgetertiary structure; strong covalent bond formed when one sulfur of one cysteine monomer bonds to the sulfur of another32
8038640752hydrocarbonorganic molecule consisting of only hydrogen and carbon33
8038640753glycosodic linkagecovalent bond formed between two monosaccharides by a dehydration synthesis reaction34
8038640754nucleotidebuilding block of a nucleic acid; five carbon sugar covalently bonded to a nitrogen base and a phosphate group35
8038640755hydroxylhydrogen bonded to oxygen bonded to an organic molecule's carbon skeleton; alcohol; polar36
8038640756carboxyloxygen double bonded to a carbon atom that is bonded to a hydroxyl group37
8038640757aminenitrogen bonded to two hydrogens and to the carbon skeleton38
8038640758sulfhydrylsulfur bonded to hydrogen in roughly the shape of a hydroxyl; cysteine39
8038640759phosphatephosphorous bonded to four oxygens; found in phospholipids and nucleotides40
8038640760carbohydrateLarge organic molecule made of monosaccharides; 1:2:1 ratio of carbon, hydrogen, and oxygen atoms; major source of energy for the human body41
8038640761lipidnon polar macromolecule made mainly from carbon and hydrogen atoms; includes fats, oils, and waxes42
8038640762proteinLarge organic molecule made of amino acids; includes carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur.43
8038640763nucleic acidLarge macromolecule made of nucleotides containing hydrogen, oxygen, nitrogen, carbon, and phosphorus; the genetic material of organisms44

AP Biology (Campbell) Chapter 10 Flashcards

6th edition

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6158193681Autotrophan organism that obtains organic food molecules without eating other organisms or substances derived from other organisms. Autotrophs use energy from the sun or from the oxidation of inorganic substances to make organic molecules from inorganic ones.0
6158193682C3 plantA plant that uses the Calvin cycle for the initial steps that incorporate CO2 into organic material, forming a three-carbon compound as the first stable intermediate.1
6158193683C4 plantA plant that prefaces the Calvin cycle with reactions that incorporate CO2 into a four-carbon compound, the end product of which supplies CO2 for the Calvin cycle.2
6158193684CAM plantA plant that uses crassulacean acid metabolism, an adaptation for photosynthesis in arid conditions, first discovered in the family Crassulaceae. Carbon dioxide entering open stomata during the night is converted into organic acids, which release CO2 for the Calvin cycle during the day, when stomata are closed.3
6158193685Carbon fixationThe initial incorporation of carbon from CO2 into an organic compound by an autotrophic organism (a plant, another photosynthetic organism, or a chemoautotrophic prokaryote).4
6158193686Cyclic electron flowA route of electron flow during the light reactions of photosynthesis that involves only photosystem I and that produces ATP but not NADPH or oxygen5
6158193687G3PThe carbohydrate produced directly from the Calvin cycle.6
6158193688HeterotrophAn organism that obtains organic food molecules by eating other organisms or their by-products.7
6158193689Light reactionsThe steps in photosynthesis that occur on the thylakoid membranes of the chloroplast and that convert solar energy to the chemical energy of ATP and NADPH, evolving oxygen in the process.8
6158193690PhotophosphorylationThe process of generating ATP from ADP and phosphate by means of a proton-motive force generated by the thylakoid membrane of the chloroplast during the light reactions of photosynthesis.9
6158193691Carotenoidany of a group of red and yellow pigments, chemically similar to carotene, contained in animal fat and some plants.10
6158193692Chlorophyll AChlorophyll a is a specific form of chlorophyll used in oxygenic photosynthesis. It absorbs most energy from wavelengths of violet-blue and orange-red light11
6158193693Chlorophyll BChlorophyll b is a form of chlorophyll. Chlorophyll b helps in photosynthesis by absorbing light energy. It is more soluble than chlorophyll a in polar solvents because of its carbonyl group. Its color is yellow, and it primarily absorbs blue light.12
6158193694CAM (Crassulacean acid metabolism)a carbon fixation pathway that evolved in some plants as an adaptation to arid conditions. In a plant using full CAM, the stomata in the leaves remain shut during the day to reduce evapotranspiration, but open at night to collect carbon dioxide (CO2).13
6158193695Mesophyllthe inner tissue (parenchyma) of a leaf, containing many chloroplasts.14
6158193696Photoautotrophcapable of synthesizing their own food from inorganic substances using light as an energy source. Green plants and photosynthetic bacteria are photoautotrophs.15
6158193697Photona discrete quantity of light energy16
6158193698Photosynthesisthe process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct.17
6158193699Photosystem Ithe mechanism that uses chlorophyll a (P700) to trap sunlight18
6158193700Photosystem IIthe mechanism that uses chlorophyll a (P680) to trap sunlight19

AP Biology Cell Structure Flashcards

A Tour of the Cell

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8352731173History of Cells (Review)1665- Robert Hooke, observed cells in cork 1833 - Robert Brown, discovered the nucleus 1838 - M.J. Schleiden, all plants are made of cells 1839 - T. Schwann, all animals are made of cells0
8352731174Cell Theory (Review)1. all living matter is composed of one or more cells 2. the cell is the structural and functional unit of life 3. all cells come from cells1
8352731175Prokaryotic Cellslack a nucleus and other membrane bounded structures domains: Bacteria and Archaea2
8352731176Eukaryotic Cellshave a nucleus and other membrane bounded structures; protists, fungi, animals and plants; much larger than prokaryotes3
8352731177Why are cells so small?cell volume-to-surface area ratios favor small size; surface area increases by a factor of n2, the volume increases by a factor of n3; small cells have a greater surface area relative to volume, this allows substances to cross the cell membrane in an efficient manner4
8352731178Basic Cell Organizationmembrane, nucleus, cytoplasm, organelles5
8352731179Cell/Plasma Membranefound in both Eukaryotes and Prokaryotes, made of phospholipid bilayer function: separates the cell from the environment; boundary layer for regulating the movement of materials in/out of a cell6
8352731180Cytoplasm/Cytosolthe "fluid" part of a cell exists in two forms: gel - thick; sol - fluid function: cell substance between the cell membrane and the nucleus7
8352731181Organelleterm means "small organ", membrane-enclosed structures (or compartment) in a cell with a specialized function; important in organizational structure of cells function: way to form compartments in cells to separate chemical reactions, keeps various enzymes separated in space8
8352731182Membrane-Bound Organellesnucleus, endoplasmic reticulum, golgi apparatus, lysosome, mitochondria, chloroplast, vacuole, vesicle (*ribosomes are not membrane bound*)9
8352731183NucleusMost conspicuous organelle; structure: usually spherical, but can be lobed or irregular in shape consists of: Nuclear Envelope, Nuclear pores, Nucleolus, Chromatin function: contains the genetic instructions in a cell10
8352731184Nuclear Envelope/Membranedouble membrane that surrounds nucleus; inner membrane supported by a protein matrix which gives the shape to the nucleus11
8352731185Nuclear Poresregular "holes" through both membranes; protein complex gives shape; allows materials in/out of nucleus12
8352731186Nucleolusdark staining area in the nucleus; 0 - 4 per nucleus; synthesizes or creates ribosomes13
8352731187Chromatin(chrom: colored; - tin: threads) DNA + Protein in a "loose" format; DNA is wrapped around proteins that forms the cell's chromosomes to make it all fit inside of the nucleus14
8352731188Ribosomesstructure: 2 subunits made of protein and rRNA, no membrane function: protein synthesis *eukaryotes and prokaryotes BOTH have ribosomes, but they are different in size* found in: cytosol (free) and on outside of rough ER (bound)15
8352731189Endomembrane Systemmembranes that are related through direct physical continuity or by the transfer of membrane segments called vesicles16
8352731190Endoplasmic Reticulum(often referred to as ER) makes up to 1/2 of the total membrane in cells structure: folded sheets or tubes of membranes; often continuous with the nuclear membrane17
8352731191Smooth ERno ribosomes function: used for lipid synthesis, carbohydrate storage, detoxification of poisons18
8352731192Rough ERwith ribosomes function: synthesizes secretory proteins to be excreted by the cell (ex: insulin)19
8352731193Golgi Apparatusstructure: parallel array of flattened cisternae (3 to 20 per cell) function: processing - modification of ER products; distribution - packaging of ER products for transport into vesicles20
8352731194Lysosomestructure: single membrane made from the Golgi apparatus function:contains hydrolytic enzymes that work best inside the acidic environment inside of the lysosome; breakdown of fats, proteins, polysaccharides, and nucleic acids, and also old or unneeded cellular materials21
8352731195Vacuolesstructure: single membrane, usually made by ER and Golgi apparatus function: depends on the organism. Plant Vacuoles- makes up 90% of the cell's volume - Water storage, ion storage, can enlarge cells and create turgor pressure, stores pigments toxins and enzymes Contractile Vacuoles- pump excess water out of protists Food Vacuoles- formed by phagocytosis and digested by lysosomes22
8352731196Mitochondriastructure: 2 membranes the inner membrane has more surface area than the outer membrane matrix: inner space Intermembrane space: area between the membranes folded into "cristae" function: cell Respiration - the release of energy from food; major location of ATP generation23
8352731197Chloroplaststructure: arranged into flattened sacs called thylakoids, some regions stacked into layers called grana which are surround by a fluid called storm; contains chlorophyll (green pigment) function: photosynthesis24
8352731198Endosymbiotic Theorymitochondria and chloroplasts were once free-living prokaryotes that got englufed by what we now know as a eukaryotic cell25
8352731199Peroxisomesspecialized metabolic compartments bounded by a single membrane function: contain enzymes that add Hydrogen atoms to O2 to generate hydrogen peroxide and enzymes that then convert it to water (ex: liver cells can detoxify alcohol by doing this)26
8352731200Cytoskeletonnetwork of protein rods and filaments in the cytoplasm; Microtubules, Microfilaments, Intermediate Filaments function: cell structure and shape; cell movement; cell division (helps build cell walls and move the chromosomes apart)27
8352731201Ciliashort, but numerous; found in respiratory passage cells function: to move cells or to sweep materials past a cell28
8352731202Flagellalong, but few; found on the end of sperm function: to move cells or to sweep materials past a cell29
8352731203Cell Wallnonliving jacket that surrounds some cells 1. plants = made of cellulose 2. bacteria = peptidoglycan 3. fungi = chitin found in: plants, prokaryotes, fungi, some protists function: cell's exoskeleton - support and protection30
8352731204Extracellular Matrix"fuzzy outer coat" of animal cells structure: made of glycoproteins and collagen function: helps glue cells together evidence suggests ECM is involved with cell behavior and cell communication31
8352731205Tight Junctionstight fusion of the membranes of adjacent cells seals off areas between cells - prevent leakage (ex: skin epithelial cells)32
8352731206Desmosomesbundles of filaments that anchor junctions between cells linking them together into strong sheets (ex: muscle cells)33
8352731207Gap Junctionsprovide cytoplasmic channels from one cell to an adjacent cell, similar to plasmodesmata in plants, materials {ions, sugars, amino acids} can pass through (ex: heart muscle)34
8352731208Plasmodesmatachannels between adjacent cell walls; allow communication between cells35

AP Biology - Biochemistry, Energy & Enzymes Flashcards

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8377150091metabolismThe sum of the building & breaking reactions occurring in cells0
8377150092catabolic pathwaysSeries of reactions that release energy by breaking down complex molecules into simpler compounds.1
8377150093anabolic pathwaysSeries of reactions that consume energy to build complicated molecules from simpler ones.2
8377150094kinetic energyEnergy associated with relative motion of objects.3
8377150095thermal energyKinetic energy associated with the random movement of molecules or atoms. (heat)4
8377150096potential energyStored energy.5
8377150097first law of thermodynamicsEnergy can be transferred and transformed, but it cannot be created or destroyed.6
8377150098entropyA measure of disorder or randomness. Tends to increase in the universe.7
8377150099second law of thermodynamicsEvery energy transfer or transformation increases the entropy of the universe.8
8377150100free energyMeasures the portion of a system's energy that can perform work when temperature and pressure are uniform throughout the system, as in a living cell.9
8377150101endergonic reactionReaction that absorbs free energy from its surroundings.10
8377150102exergonic reactionReaction that proceeds with a net release of free energy.11
8377150103energy couplingThe use of an exergonic process to drive an endergonic one.12
8377150104ATP (adenosine triphosphate)Composed of a sugar ribose, nitrogenous base adenine, and a chain of three phosphate groups bonded to it.13
8377150105phosphorylationThe metabolic process of introducing a phosphate group into an organic molecule.14
8377150106catalystA chemical agent that speeds up a reaction without being consumed by the reaction.15
8377150107enzymeProtein that speeds up reactions. Typically end in "ase" (ex. Peroxidase, Lipase)16
8377150108activation energyThe amount of energy needed to push the reactants over an energy barrier.17
8377150109enzyme-substrate complexWhen an enzyme binds to its substrate, it forms:18
8377150110active siteA pocket or groove on the surface of the enzyme where a substrate can bind.19
8377150111induced fit modelStates that the enzyme and substrate undergo conformational changes to interact fully with one another (as opposed to "Lock & Key"20
8377150112cofactorInorganic metal ion helpers that may be bound tightly to the enzyme as a permanent resident, or may bind loosely and reversibly along with the substrate.21
8377150113coenzymeNon-protein Organic helpers that may be bound tightly to the enzyme as a permanent resident, or may bind loosely and reversibly along with the substrate.22
8377150114competitive inhibitorsReduce the productivity of enzymes by blocking substrates from entering active sites.23
8377150115noncompetitive inhibitorsImpede enzymatic reactions by binding to another part of the enzyme (other than the active site).24
8377150116feedback inhibition/negative feedbackA metabolic pathway is switched off by the inhibitory binding of its end product to an enzyme that acts early in the pathway.25
8377150117saturated enzymeDescribes an enzyme's maximum activity when every active site is being used.26
8377150118substrateThe reactant that an enzyme binds with to form product.27
8377150119Chemical EnergyPotential energy trapped in molecular bonds.28
8377150120Spontaneous ReactionWhen a reaction doesn't require energy to proceed it is said to be this - doesn't mean it will be FAST.29
8377150121ionic bondbond resulting from a transfer of electrons causing an attraction between oppositely charged ions30
8377150122covalent bondA chemical bond that involves sharing a pair of electrons between atoms in a molecule31
8377150123nonpolara molecule in which all atoms have the same electronegativity and the electron distribution is equal, hydrophobic32
8377150124polarmolecule in which the positive and negative charges are separated and unequal, hydrophilic33
8377150125buffermixture that can react with an acid or a base to maintain the pH within a specific range34
8377150126isomerorganic compounds with the same molecular formula but different structure35
8377150127monosaccharideA single sugar molecule such as glucose or fructose, the simplest type of sugar; monomer of carbohydrates36
8377150128disaccharideA double sugar, consisting of two monosaccharides joined by dehydration synthesis.37
8377150129dehydration synthesisA chemical reaction in which two molecules are bonded together with the removal of a water molecule.38
8377150130hydrolysisBreaking down complex molecules (polymers) by the chemical addition of water.39
8377150131polysaccharideA polymer of many monosaccharides, formed by dehydration reactions.40
8377150132cellulosestructural polysaccharide that makes up plant cell walls41
8377150133starchStorage polysaccharide found in plant cells that consists entirely of glucose monomers42
8377150134glycerolThree-carbon compound with three hydroxyl groups; fatty acids are covalently bonded to it to make fats and oils.43
8377150135fatty acidhydrocarbon chain with a carboxyl group at one end; majority portion in lipids44
8377150136saturated fatA lipid made from fatty acids that have no double bonds between carbon atoms; solid at room temperature and found in animal fats, lards, and dairy products.45
8377150137unsaturated fatA lipid made from fatty acids that have at least one double bond between carbon atoms; liquid at room temperature and found in vegetable oils, nuts, and seeds.46
8377150138steroidlipid with a four fused ring structure; cholesterol and testosterone47
8377150139peptide bondbond creating amino acid chains or polymers48
8377150140primary structurelinear sequence of amino acids; peptide bonds49
8377150141secondary structureEither an alpha helix or beta pleated sheet formed with hydrogen bonds50
8377150142tertiary structure3D shape of single polypeptide due to interactions of the side chains using hydrogen bonding, ioning bonding and disulphide bridges51
8377150143quaternary structureThe shape resulting from the association of two or more polypeptide subunits.52
8377150144alpha helixsecondary structure form of a protein; human hair (keratin)53
8377150145beta pleated sheetsecondary structure form of a protein; spider webs and silk54
8377150146functional groupgroup of atoms within a molecule that interacts in predictable ways with other molecules55
8377150147cohesionattraction between molecules of the same substance; Water molecules sticking to each other.56
8377150148polymerlong molecule consisting of many similar or identical monomers linked together57
8377150149monomerbuilding block for polymers; only made by producers58
8377150150adhesionattraction between different kinds of molecules; Water molecules sticking to other surfaces.59
8377150151surface tensionmeasure of how difficult it is to stretch or break the surface of a liquid60
8377150152van der Waals interactionstertiary structure; weak attractions between molecules or parts of molecules that result from localized charge fluctuations61
8377150153disulfide bridgetertiary structure; strong covalent bond formed when one sulfur of one cysteine monomer bonds to the sulfur of another62
8377150154hydrocarbonorganic molecule consisting of only hydrogen and carbon63
8377150155glycosodic linkagecovalent bond formed between two monosaccharides by a dehydration synthesis reaction64
8377150156nucleotidebuilding block of a nucleic acid; five carbon sugar covalently bonded to a nitrogen base and a phosphate group65
8377150157hydroxylhydrogen bonded to oxygen bonded to an organic molecule's carbon skeleton; alcohol; polar66
8377150158carboxyloxygen double bonded to a carbon atom that is bonded to a hydroxyl group67
8377150159aminenitrogen bonded to two hydrogens and to the carbon skeleton68
8377150160sulfhydrylsulfur bonded to hydrogen in roughly the shape of a hydroxyl; cysteine69
8377150161phosphatephosphorous bonded to four oxygens; found in phospholipids and nucleotides70
8377150162carbohydrateLarge organic molecule made of monosaccharides; 1:2:1 ratio of carbon, hydrogen, and oxygen atoms; major source of energy for the human body71
8377150163lipidnon polar macromolecule made mainly from carbon and hydrogen atoms; includes fats, oils, and waxes72
8377150164proteinLarge organic molecule made of amino acids; includes carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur.73
8377150165nucleic acidLarge macromolecule made of nucleotides containing hydrogen, oxygen, nitrogen, carbon, and phosphorus; the genetic material of organisms74

AP Biology Molecular genetic Flashcards

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6142156632Griffith's bacterial transformation-____________(answer is guys name and name of experiment) experiment in 1927 revealed that harmless bacteria have the ability to transform themselves into virulent bacteria by absorbing some genetic factor from another bacterium0
6142166432Avery, Macleod, and McCarty-isolated and purified Griffith's bacterial transformation factor and proved that it was, in fact, DNA1
6142173872Hershey and Chase-carried out experiments that lent strong support to the theory that DNA is the genetic material -Tagged bacteriophages with isotopes P32 and S35 -proved that DNA from the phage nucleus, not protein from the phage coat, was infecting bacteria2
6142186471Rosalind Franklin-while working in Maurice Wilkin's lab, she carried out X-ray crystallography analysis of DNA that showed DNA to be a helix3
6142197771Watson and Crick-1953-proposed the double-helix structure of DNA -Received the Nobel Prize for their work -hypothesized that DNA might replicate by semiconservative replication4
6142203810Meselson and Stahl-proved that DNA replicates in a semiconservative fashion, confirming Watson and Crick's hypothesis -Cultured bacteria in a medium containing heavy nitrogen(N15) and then a medium containing light nitrogen(N14); after extracting the DNA, they demonstrated that the replicated DNA consisted of one heavy strand and one light strand5
6142220935semiconservative replication-DNA replicates in a semiconservative fashion, as proven by Meselson and Stahl6
6142227309DNA-__________(abbreviation is the answer)is a polymer, repeating sequences of nucleotides: A, T, C, and G -double helix consists of 2 strands running in opposite directions: 5' to 3' and 3' to 5'7
6142509960RNA-ribonucleic acid -consists of the sugar ribose; phosphate; a nitrogen base: adenine(A), cytosine(c), guanine(G), or uracil(U)8
6142518266mRNA-relays the DNA message to the ribosome9
6142520368tRNA-cloverleaf shape, carries amino acids to mRNA at the ribosome10
6142524096SiRNA-small interfering RNA -blocks gene transmission -small interfering RNA11
6142526333miRNA-degrades specific mRNA molecules or blocks them12
6142531719nucleotideconsists of phosphate, a 5 carbon sugar(deoxyribose or ribose), and a nitrogen base(A, T, C, G, or U found in RNA, not DNA)13
6142540894DNA replication-occurs during the S phase of the cell cycle in semiconservative fashion -begins at multiple ORIGINS OF REPLICATION in eukaryote14
6142546108RNA primer-short RNA strand -is the starting point for DNA synthesis=initiation site of replication15
6142550762replication forksreplication proceeds in both directions from a replication bubble, forming 2 _____________16
6142555346DNA polymeraseforms 2 new strands in the 5' to 3' direction17
6142560272leading strandforms continuously toward the fork18
6142562412lagging strandforms AWAY from the replication fork in discontinous segments called Okazaki fragments19
6142565599okazaki fragmentsdiscontinuous segments20
6142568916helicaseuntwists double helix at replication fork21
6142570951single-stranded binding proteinsscaffolding that holds two strands of DNA apart as they are copied22
6142574469topoisomeraselessen tension on tightly wound DNA23
6142577090DNA ligasejoins okazaki fragments together24
6142579218primaseenzyme that joins RNA nucleotides to make primer25
6142581089DNA polymerase-adds nucleotides only to 3' end of an existing strand(RNA primer) -proofreads new strands and carries out mismatch repair26
6142596088transcriptionthe process by which DNA makes RNA -consists of 3 stages: initiation, elongation, termination -initial transcript is the unprocessed version of mRNA27
6142597787transcription unitstretch of DNA that is transcribed28
6142597789initiation-first step of RNA transcription -RNA polymerase binds to DNA at the promoter29
6142600218elongation-second step of RNA transcription -RNA polymerase adds nucleotides to the 3' end of a growing chain30
6142603480termination-third and last step of RNA transcription -stops after the termination sequence31
6142621847RNA processing-occurs before the newly transcribed mRNA(initial transcript) leaves the nucleus -noncoding regions of mRNA are removed by snRNPS and spliceosomes -5' cap of modified guanine nucleotide is added to 5' end of mRNA -Poly-A tail is added to 3' end of mRNA -Both 5' cap and poly-A tail protect the mRNA strand from enzymatic degradation and help the strand attach to the ribosome32
6142628544intronsnoncoding regions of mRNA33
6142643455exonsthe mRNA strand that leaves the nucleus is much smaller than the inital transcript and contains only____________.34
6142654333translation-the process by which a codon sequence of mRNA is made into an amino acid sequence -GTP(guanosine triphosphate) supplies energy for this process -64 codons on mRNA are translated into 20 amino acids -consists of 3 stages: initiation, elongation, and termination35
6142666620amino acids, tRNA, mRNA_______________ from the cytoplasm are carried by _________ to the codons of ___________ at the ribosomes36
6142668922AUG-the "start reading" codon that begins every mRNA strand is?(Three nitrogenous bases)37
6142676853UAA, UAG, and UGA"stop" codons that end every mRNA strand are called?38
6142683116wobble-the relaxation of base-pairing rules for the third nucleotide -EX; UCU, UCC, UCA, and UCG all code for the same amino acid, serine -therefore, a mutation in a gene does not necessarily express itself as a change in amino acid sequence39
6142693880introns-intervening sequence -do not code for any protein -are removed during RNA processing -found in eukaryotic DNA; not present in bacterial DNA40
6142701235exons-expressed sequences=genes -code for polypeptide41
6142707202alternative splicing-different RNA molecules are produced from the same transcript depending on which RNA segments are treated as introns and which as exons -regulatory proteins control the intron-exon choice by binding to regulatory sequences within the primary transcript42
6143376414chromatin structure-binding histones more loosely or tightly to the nucleosome makes the DNA more or less accessible for transcription43
6143380515methylation of DNaadding methyl groups(CH4) to DNA silences the genes44
6143382976epigenetic inheritancereversible changes to the DNA that do not change the actual sequence45
6143385456regulation at transcription levelalternative slicing during RNA processing alters which segments are edited out of the final RNA transcript46
6143388499degradation of mRNAthe length of time that a specific molecule of mRNA remains in the cytoplasm varies and is controlled47
6143407951TATA box-critical to the process of transcription -area within the PROMOTER that mediates binding of transcription factors and RNA polymerase to DNA -directs RNA polymerase to the initiation site48
6143416769genetics of viruses-a virus is a parasite that can only live inside another cell; it commandeers the host cell's machinery to transcribe and translate all the proteins necessary to replicate new viruses49
6143421903viruses__________ are enclosed in a protein called a capsid or viral envelope50
6143424305capsida protein coat that cloaks the virus51
6143427374host rangethe range of organisms that a virus normally infects52
6143429677bacteriophage-phage virus -most complex and best studied virus -it attacks bacteria53
6143432410retroviruscontains RNA(not DNA) -produces new DNA, complementary DNA(c-DNA) using the enzyme reverse transcriptase54
6143434846transduction-leads to genetic recombination -process by which phage viruses acquire bits of foreign DNA as they infect different bacterial cells and transfer them to other cells as part of the infection process -two types of transduction: generalized and restricted55
6143442923generalized transductionmoves random pieces of DNA from one cell to another56
6143444402restricted transductiontransfers specific pieces of DNA during lysogenic cycle57
6143447164lytic cyclevirus infects and takes over a cell, replicates itself, causes the cell to burst, and releases a new generation of infectious viruses58
6143456013lysogenic cyclevirus infects cell but replicates without destroying the cell; it becomes a PROPHAGE, integrated into a specific site in the host's DNA; it can revert to the lytic cycle when triggered by some environmental factor59
6143493549plasmid-foreign, small, circular, self-replicating DNA molecule that inhabits bacteria and can express itself through the host bacterium -one bacterium can contain many ___________60
6143498170F plasmid-for fertility -contains genes fro production of a pilus, a cytoplasmic bridge connecting two cells that allows DNA to move from one cell to another(a primitive form of sexual reproduction called conjugation)61
6143503244R plasmid-confers resistance to an antibiotic on a host bacterium; can be transferred from one cell to another by conjugation62
6143506347operon-an important model for gene regulation -found in prokaryotes only -two types: inducible(Lac) and repressible(tryptophan)63
6143599692lac operonis "off" unless induced to turn on64
6143601079tryptophan operonis "on" unless turned off(repressed)65
6143608464promoterbinding site for RNA polymerase66
6143608465operatorbinding site for repressor67
6143609832repressorbinds to operator and prevents RNA polymerase from binding to promoter; is allosteric68
6143612458regulator geneproduces repressor69
6143612459induceran isomer of lactose, acts as allosteric effector for the repressor, causing the repressor to change its shape70
6143615377prions-infectious, misfolded proteins that cause severe brain diseases: -scrapie in sheep -mad cow disease in cattle -creutzfeldt-jakob disease in humans -prions spread disease by causing other proteins to misfold71
6143646185transposons-transposable genetic elements=jumping genes-move around the genome -discovered by Barbara McClintock in corn -two classes of transposons: insertion sequences and complex transposon72
6143660717insertion sequences-consists of only one gene that codes for transposase, the enzyme responsible for moving the gene; these can cause mutations if they land within a region of DNA that regulates gene expression73
6143667970complex transposonincludes the insertion sequence+ other genes74
6143670518human genome-consists of about 25,000 genes and 3 billion base pairs -97%=noncoding regions75
6143672464regulatory sequencescontrols gene expression76
6143673735intronsintervening sequences; are excised from the initial transcript during RNA processing77
6143677163tandem repeatsshort sequences of DNA that repeat over and over Ex: make up telomeres78
6143679029satellite DNAshort sequences of DNA that repeat as many79
6143684448alu sequencea DNA sequence about 300 base pairs long, 5% of human DNA80
6143685918telomeres-special nucleotide sequences found at the ends of eukaryotic DNA -consists of a short nucleotide sequence, TTAGGG, repeated 100-1000 times -protects organisms's genes from being eroded through repeated rounds of DNA replication -most body cells do not have_______ and the DNA of older cells is shorter than thatof younger cells81
6143694083telomeraseenzyme that catalyzes the lengthening of telomeres, is present in normal fetal and cancer cells82
6143696773p-53 gene-"guardian of the genome" -a transcription factor for several genes -turns on genes involved in DNA repair -If Dna damage is irreparable, p-53 activates suicide genes, which cause cell death-apoptosis83
6143702310genetic engineering-produces recombinant DNA -combines DNA from two sources into one molecule -used in biotechnology -gene cloning -tools used: restriction enzymes, gel electrophoresis, DNA probes, RFLPS, or cDNA84
6143718897gene therapyreplacing a nonfunctioning gene in a person's cells with a correctly function one85
6143721543uses for genetic engineering-gene therapy -produces human protein, such as insulin, for therapeutic use -prepare multiple copies of genes for study(cells usually have only one or two copies of a gene -bacteria have been engineered to digest oil in oil spills86
6143747846restriction enzymes-discovered in late 1960s in bacteria, where they are a defense against infection by viruses -basic tool of biotechnology; called molecular scissors -cut DNA at specific recognition sites87
6143779944recognition fragmentsresulting fragments of DNA from recognition sites88
6143796947gel electrophoresis-technique that separates molecules of DNA in an agarose gel in an electricc field -DNA is digested with restriction enzymes before being run in a gel -the smaller the molecule of DNA, the faster and farther it runs through the gel -DNA(which is negative) runs from the cathode(-) to the anode(+) -DNA in gel is stained and viewed under UV light -In order to do finer separation of similarly sized pieces of DNA, the concentration of the agarose gel can be increased -also used to separate proteins89
6143823251gene cloning method-isolate gene of interest -insert that gene into a PLASMID -insert the plasmid into a CLONING VECTOR, such as a bacterium; to accomplish this, the bacterium must be made competent -As the bacterium reproduces by fission, the gene is copied or cloned -identify the bacteria that contain the selected genes and harvest them -Human insulin and growth hormone are made for therapeutic use with this technique90
6143839450making a cell component-technique to enable a cell to uptake a plasmid -carried out by a series of drastic temperature changes(heat shock) in the presence of Ca2+ to disrupt the cell membrane91
6143844978DNA probe-radioactively labeled single strand of nucleic acid -used to tag a specific sequence in a DNA sample -DNA is denatured or melted(two strands are separated, and the probe binds to the complementary sequence wherever it occurs -used to identify a person who carries an inherited gene mutation, such as sickle cell anemia or huntington's disease92
6143879987complementary DNA-DNA made from RNA using reverse transcriptase -Carries a complete coding sequence with no introns -can be placed in a cloning vector for genetic engineering.93
6143883578RFLP-restriction fragment length polymorphisms -differences in noncoding regions of DNA that vary across the population -scattered throughout the genome -are genetic markers tha produce a DNA fingerprint -No two people(except identical twins) have the same RFLP pattern -inherited in simple Mendelian fashion94
6143889532PCR(polymerase chain reaction)-cell-free, autopmated technique by which a tiny piece of DNA can be rapidly copied(amplified) -Dna in saliva from licking a stamp can be amplified into billions of copies in a few hours -Requires Taq enzymes(Taq polymerase) -limitation of technique: some of the sequence of DNA to be amplified must be known in advance in order to make necessary primers95
6143897769Taq enzymes(Taq polymerase)-Taq=thermus aquaticus -enzyme that is extracted from extremophile bacteria that live in hot springs -heat stable at 100C -required for PCR technique96

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